Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Mar;133(3):1393–1400. doi: 10.1128/jb.133.3.1393-1400.1978

Ultrastructure of Rhizobium japonicum in relation to its attachment to root hairs.

A K Bal, S Shantharam, S Ratnam
PMCID: PMC222177  PMID: 565352

Abstract

In Rhizobium japonicum strain Nitragin 61A76, morphologically distinct types of bacteria were found to occur in yeast extract-mannitol broth cultures, at both mid-log and stationary phases. Of these only the capsular form, characterized by a smooth cell envelope, storage granules (glycogen and poly-beta-hydroxybutyric acid), and an amorphous extracellular capsule, bound soybean lectin. The binding site was localized in the capsular material. Less than 1% of the bacterial population differentiated into these capsular forms, which were also able to attach to the soybean root hair surface.

Full text

PDF
1394

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bal A. K., Verma D. P., Byrne H., Maclachlan G. A. Subcellular localization of cellulases in auxin-treated pea. J Cell Biol. 1976 Apr;69(1):97–105. doi: 10.1083/jcb.69.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Björndal H., Erbing C., Lindberg B., Fåhraeus G., Ljunggren H. Studies on an extracellular polysaccharide from Rhizobium meliloti. Acta Chem Scand. 1971;25(4):1281–1286. doi: 10.3891/acta.chem.scand.25-1281. [DOI] [PubMed] [Google Scholar]
  3. Bohlool B. B., Schmidt E. L. Immunofluorescent polar tips of Rhizobium japonicum: possible site of attachment or lectin binding. J Bacteriol. 1976 Mar;125(3):1188–1194. doi: 10.1128/jb.125.3.1188-1194.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bohlool B. B., Schmidt E. L. Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis. Science. 1974 Jul 19;185(4147):269–271. doi: 10.1126/science.185.4147.269. [DOI] [PubMed] [Google Scholar]
  5. Chaudhari A. S., Bishop C. T., Dudman W. F. Structural studies on the specific capsular polysaccharide from Rhizobium trifolii, TA-1. Carbohydr Res. 1973 Jun;28(2):221–231. doi: 10.1016/s0008-6215(00)82778-0. [DOI] [PubMed] [Google Scholar]
  6. Cheng K. J., Costerton J. W. Ultrastructure of cell envelopes of bacteria of the bovine rumen. Appl Microbiol. 1975 Jun;29(6):841–849. doi: 10.1128/am.29.6.841-849.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clasener H. Pathogenicity of the L-phase of bacteria. Annu Rev Microbiol. 1972;26:55–84. doi: 10.1146/annurev.mi.26.100172.000415. [DOI] [PubMed] [Google Scholar]
  8. Costerton J. W., Damgaard H. N., Cheng K. J. Cell envelope morphology of rumen bacteria. J Bacteriol. 1974 Jun;118(3):1132–1143. doi: 10.1128/jb.118.3.1132-1143.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Costerton J. W., Ingram J. M., Cheng K. J. Structure and function of the cell envelope of gram-negative bacteria. Bacteriol Rev. 1974 Mar;38(1):87–110. doi: 10.1128/br.38.1.87-110.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Currier W. W., Strobel G. A. Chemotaxis of Rhizobium spp. to a Glycoprotein Produced by Birdsfoot Trefoil Roots. Science. 1977 Apr 22;196(4288):434–436. doi: 10.1126/science.196.4288.434. [DOI] [PubMed] [Google Scholar]
  11. DUDMAN W. F. GROWTH AND EXTRACELLULAR POLYSACCHARIDE PRODUCTION BY RHIZOBIUM MELILOTI IN DEFINED MEDIUM. J Bacteriol. 1964 Sep;88:640–645. doi: 10.1128/jb.88.3.640-645.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dazzo F. B., Brill W. J. Receptor site on clover and alfalfa roots for Rhizobium. Appl Environ Microbiol. 1977 Jan;33(1):132–136. doi: 10.1128/aem.33.1.132-136.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Dudman W. F. Capsultation in Rhizobium species. J Bacteriol. 1968 Mar;95(3):1200–1201. doi: 10.1128/jb.95.3.1200-1201.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Dudman W. F. Detection of acidic polysaccharides in gels by DEAE-dextran. Anal Biochem. 1972 Apr;46(2):668–673. doi: 10.1016/0003-2697(72)90338-7. [DOI] [PubMed] [Google Scholar]
  15. Hubbell D. H., Elkan G. H. Correlation of physiological characteristics with nodulating ability in Rhizobium japonicum. Can J Microbiol. 1967 Feb;13(2):235–241. doi: 10.1139/m67-030. [DOI] [PubMed] [Google Scholar]
  16. Irvin R. T., Chatterjee A. K., Sanderson K. E., Costerton J. W. Comparison of the cell envelope structure of a lipopolysaccharide-defective (heptose-deficient) strain and a smooth strain of Salmonella typhimurium. J Bacteriol. 1975 Nov;124(2):930–941. doi: 10.1128/jb.124.2.930-941.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MURRAY R. G., STEED P., ELSON H. E. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. doi: 10.1139/m65-072. [DOI] [PubMed] [Google Scholar]
  18. Pate J. L., Ordal E. J. The fine structure of Chondrococcus columnaris. 3. The surface layers of Chondrococcus columnaris. J Cell Biol. 1967 Oct;35(1):37–51. doi: 10.1083/jcb.35.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Perry M. M. Identification of glycogen in thin sections of amphibian embryos. J Cell Sci. 1967 Jun;2(2):257–264. doi: 10.1242/jcs.2.2.257. [DOI] [PubMed] [Google Scholar]
  20. Planqué K., Kijne J. W. Binding of pea lectins to a glycan type polysaccharide in the cell walls of Rhizobium leguminosarum. FEBS Lett. 1977 Jan 15;73(1):64–66. doi: 10.1016/0014-5793(77)80016-1. [DOI] [PubMed] [Google Scholar]
  21. Roland J. C., Lembi C. A., Morré D. J. Phosphotungstic acid-chromic acid as a selective electron-dense stain for plasma membranes of plant cells. Stain Technol. 1972 Jul;47(4):195–200. doi: 10.3109/10520297209116484. [DOI] [PubMed] [Google Scholar]
  22. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  23. Sundberg L., Porath J., Aspberg K. Simultaneous isolation of trypsin inhibitor and anti-A phytohemagglutinin from Vicia cracca by means of biospecific adsorption. Biochim Biophys Acta. 1970 Nov 17;221(2):394–395. doi: 10.1016/0005-2795(70)90285-0. [DOI] [PubMed] [Google Scholar]
  24. Wolpert J. S., Albersheim P. Host-symbiont interactions. I. The lectins of legumes interact with the o-antigen-containing lipopolysaccharides of their symbiont Rhizobia. Biochem Biophys Res Commun. 1976 Jun 7;70(3):729–737. doi: 10.1016/0006-291x(76)90653-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES