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. 1978 Aug;135(2):354–362. doi: 10.1128/jb.135.2.354-362.1978

Effect of near-UV light on Escherichia coli in the presence of 8-methoxypsoralen: wavelength dependency of killing, induction of prophage, and mutation.

H Fujita, K Suzuki
PMCID: PMC222391  PMID: 355223

Abstract

Wavelength dependency of photo-inactivation and photoinduced reverse mutation of Escherichia coli sensitized with 8-methoxypsoralen, and wavelength dependency of photoinduction of lambda prophage from the sensitized lysogen were measured in a range of 298 to 400 nm. The most efficient sensitization for these biological effects was observed between 320 and 340 nm. In the presence of 8-methyoxypsoralen, the induced mutation frequency per lethal hit was highest of 298 nm in the range examined and was gradually decreased with increasing wavelength to a minimum frequency at 345 nm. This finding may be a reflection of the production of more than one type of lesions which have different efficiencies for mutation compared with the killing efficiency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderson T., Scott B. R. The photosensitising effect of 8-methoxypsoralen on the inactivation and mutation of Aspergillus conidia by near ultraviolet light. Mutat Res. 1970 Jun;9(6):569–578. doi: 10.1016/0027-5107(70)90102-8. [DOI] [PubMed] [Google Scholar]
  2. Bordin F., Carlassare F., Baccichetti F., Anselmo L. DNA repair and recovery in Escherichia coli after psoralen and angelicin photosensitization. Biochim Biophys Acta. 1976 Oct 18;447(3):249–259. doi: 10.1016/0005-2787(76)90048-4. [DOI] [PubMed] [Google Scholar]
  3. Brooks K., Clark A. J. Behavior of lambda bacteriophage in a recombination deficienct strain of Escherichia coli. J Virol. 1967 Apr;1(2):283–293. doi: 10.1128/jvi.1.2.283-293.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carbera-Juárez E., Setlow J. K. Formation of a thymine photoproduct in transforming DNA by near ultraviolet irradiation. Biochim Biophys Acta. 1977 Mar 18;475(2):315–322. doi: 10.1016/0005-2787(77)90022-3. [DOI] [PubMed] [Google Scholar]
  5. Cole R. S. Inactivation of Escherichia coli, F' episomes at transfer, and bacteriophage lambda by psoralen plus 360-nm light: significance of deoxyribonucleic acid cross-links. J Bacteriol. 1971 Sep;107(3):846–852. doi: 10.1128/jb.107.3.846-852.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cole R. S., Levitan D., Sinden R. R. Removal of psoralen interstrand cross-links from DNA of Escherichia coli: mechanism and genetic control. J Mol Biol. 1976 May 5;103(1):39–59. doi: 10.1016/0022-2836(76)90051-6. [DOI] [PubMed] [Google Scholar]
  7. Cole R. S. Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells. Biochim Biophys Acta. 1970 Sep 17;217(1):30–39. doi: 10.1016/0005-2787(70)90119-x. [DOI] [PubMed] [Google Scholar]
  8. Cole R. S. Psoralen monoadducts and interstrand cross-links in DNA. Biochim Biophys Acta. 1971 Nov 29;254(1):30–39. doi: 10.1016/0005-2787(71)90111-0. [DOI] [PubMed] [Google Scholar]
  9. Dall'Acqua F., Marciani S., Rodighiero G. Photoreactivity (3655 Angstrom) of various skin-photosensitizing furocoumarins with nucleic acids. Z Naturforsch B. 1969 Mar;24(3):307–314. doi: 10.1515/znb-1969-0309. [DOI] [PubMed] [Google Scholar]
  10. Dall'Acqua F., Marciani S., Rodighiero G. The action spectrum of xanthotoxin and bergapten for the photoreaction with native DNA. Z Naturforsch B. 1969 Jun;24(6):667–671. [PubMed] [Google Scholar]
  11. Drake J. W., McGuire J. Properties of r mutants of bacteriophage T4 photodynamically induced in the presence of thiopyronin and psoralen. J Virol. 1967 Apr;1(2):260–267. doi: 10.1128/jvi.1.2.260-267.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Igali S., Bridges B. A., Ashwood-Smith M. J., Scott B. R. Mutagenesis in Escherichia coli. IV. Photosensitization to near ultraviolet light by 8-methoxypsoralen. Mutat Res. 1970 Jan;9(1):21–30. doi: 10.1016/0027-5107(70)90067-9. [DOI] [PubMed] [Google Scholar]
  14. Kondo S., Ichikawa H., Iwo K., Kato T. Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics. 1970 Oct;66(2):187–217. doi: 10.1093/genetics/66.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kondo S., Kato T. Action spectra for photoreactivation of killing and mutation to prototrophy in U.V.-sensitive strains of Escherichia Coli possessing and lacking photoreactivating enzyme. Photochem Photobiol. 1966 Nov-Dec;5(11):827–837. doi: 10.1111/j.1751-1097.1966.tb05929.x. [DOI] [PubMed] [Google Scholar]
  16. LWOFF A. Lysogeny. Bacteriol Rev. 1953 Dec;17(4):269–337. doi: 10.1128/br.17.4.269-337.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Scott B. R., Alderson T. The random (non-specific) forward mutational response of gene loci in Aspergillus conidia after photosensitisation to near ultraviolet light (365 nm) by 8-methoxypsoralen. Mutat Res. 1971 May;12(1):29–34. doi: 10.1016/0027-5107(71)90069-8. [DOI] [PubMed] [Google Scholar]
  18. Seki T., Nozu K., Kondo S. Differential causes of mutation and killing in Escherichia coli after psoralen plus light treatment: monoadducts and cross-links. Photochem Photobiol. 1978 Jan;27(1):19–24. doi: 10.1111/j.1751-1097.1978.tb07559.x. [DOI] [PubMed] [Google Scholar]
  19. Suzuki K., Fujita H., Yanagisawa F., Kitakami M. Action spectrum for the photo-inactivation of lambda and T-4 bacteriophages sensitized with 8-methoxypsoralen. Photochem Photobiol. 1977 Jul;26(1):49–51. doi: 10.1111/j.1751-1097.1977.tb07447.x. [DOI] [PubMed] [Google Scholar]
  20. Suzuki K., Yanagisawa F., Fujita H., Ohkido M. Inactivation of T4 bacteriophage by near ultraviolet light in the presence of 8-methoxypsoralen. J Dermatol. 1978 Feb;5(1):15–19. doi: 10.1111/j.1346-8138.1978.tb01043.x. [DOI] [PubMed] [Google Scholar]
  21. Swanbeck G., Thyresson M. Induction of respiration-deficient mutants in yeast by psoralen and light. J Invest Dermatol. 1974 Aug;63(2):242–244. doi: 10.1111/1523-1747.ep12680024. [DOI] [PubMed] [Google Scholar]
  22. Tyrrell R. M. Induction of pyrimidine dimers in bacterial DNA by 365 nm radiation. Photochem Photobiol. 1973 Jan;17(1):69–73. doi: 10.1111/j.1751-1097.1973.tb06334.x. [DOI] [PubMed] [Google Scholar]
  23. Tyrrell R. M., Ley R. D., Webb R. B. Induction of single-strand breaks (alkali-labile bonds) in bacterial and phage DNA by near UV (365 nm) radiation. Photochem Photobiol. 1974 Nov;20(5):395–398. doi: 10.1111/j.1751-1097.1974.tb06593.x. [DOI] [PubMed] [Google Scholar]
  24. WITKIN E. M. PHOTOREVERSAL AND "DARK REPAIR" OF MUTATIONS TO PROTOTROPHY INDUCED BY ULTRAVIOLET LIGHT IN PHOTOREACTIVABLE AND NON-PHOTOREACTIVABLE STRAINS OF ESCHERICHIA COLI. Mutat Res. 1964 May;106:22–36. doi: 10.1016/0027-5107(64)90049-1. [DOI] [PubMed] [Google Scholar]
  25. Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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