Abstract
Caulobacter crescentus wild-type strain CB13 is unable to utilize galactose as the sole carbon source unless derivatives of cyclic AMP are present. Spontaneous mutants have been isolated which are able to grow on galactose in the absence of exogenous cyclic nucleotides. These mutants and the wild-type strain were used to determine the pathway of galactose catabolism in this organism. It is shown here that C. crescentus catabolizes galactose by the Entner-Duodoroff pathway. Galactose is initially converted to galactonate by galactose dehydrogenase and then 2-keto-3-deoxy-6-phosphogalactonate aldolase catalyzes the hydrolysis of 2-keto-3-deoxy-6-phosphogalactonic acid to yield triose phosphate and pyruvate. Two enzymes of galactose catabolism, galactose dehydrogenase and 2-keto-3-deoxy-6-phosphogalactonate aldolase, were shown to be inducible and independently regulated. Furthermore, galactose uptake was observed to be regulated independently of the galactose catabolic enzymes.
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Selected References
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- DE LEY J., DOUDOROFF M. The metabolism of D-galactose in Pseudomonas saccharophila. J Biol Chem. 1957 Aug;227(2):745–757. [PubMed] [Google Scholar]
- ENTNER N., DOUDOROFF M. Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J Biol Chem. 1952 May;196(2):853–862. [PubMed] [Google Scholar]
- Kemp M. B., Hegeman G. D. Genetic control of the beta-ketoadipate pathway in Pseudomonas aeruginosa. J Bacteriol. 1968 Nov;96(5):1488–1499. doi: 10.1128/jb.96.5.1488-1499.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurn N., Shapiro L., Agabian N. Effect of carbon source and the role of cyclic adenosine 3',5'-monophosphate on the Caulobacter cell cycle. J Bacteriol. 1977 Sep;131(3):951–959. doi: 10.1128/jb.131.3.951-959.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurn N., Shapiro L. Effect of 3':5'-cyclic GMP derivatives on the formation of Caulobacter surface structures. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3303–3307. doi: 10.1073/pnas.73.9.3303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leidigh B. J., Wheelis M. L. The clustering on the Pseudomonas putida chromosome of genes specifying dissimilatory functions. J Mol Evol. 1973 Nov 27;2(4):235–242. doi: 10.1007/BF01654092. [DOI] [PubMed] [Google Scholar]
- Ornston L. N. Regulation of catabolic pathways in Pseudomonas. Bacteriol Rev. 1971 Jun;35(2):87–116. doi: 10.1128/br.35.2.87-116.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg S. L., Hegeman G. D. Clustering of functionally related genes in Pseudomonas aeruginosa. J Bacteriol. 1969 Jul;99(1):353–355. doi: 10.1128/jb.99.1.353-355.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapiro L., Agabian-Keshishian N., Hirsch A., Rosen O. M. Effect of dibutyryladenosine 3':5'-cyclic monophosphate on growth and differentiation in Caulobacter crescentus. Proc Natl Acad Sci U S A. 1972 May;69(5):1225–1229. doi: 10.1073/pnas.69.5.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shedlarski J. G., Jr Glucose-6-phosphate dehydrogenase from Caulobacter crescentus. Biochim Biophys Acta. 1974 Jul 17;358(1):33–43. doi: 10.1016/0005-2744(74)90255-1. [DOI] [PubMed] [Google Scholar]
- Wheelis L. The genetics of dissimilarity pathways in Pseudomonas. Annu Rev Microbiol. 1975;29:505–524. doi: 10.1146/annurev.mi.29.100175.002445. [DOI] [PubMed] [Google Scholar]
- Wheelis M. L., Stanier R. Y. The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics. 1970 Oct;66(2):245–266. doi: 10.1093/genetics/66.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]