Abstract
A previously described mutant of Escherichia coli which lacks 4-thiouridine in its tRNA was here shown to be deficient in factor A, one of the two proteins responsible for this thiolation of uridine. Addition of exogenous factor A restored the thiolating ability of extracts prepared from the mutant. The activities of the two thiolation proteins were governed by genes at two widely separated positions on the chromosome, as determined with F-prime merodiploids. The site governing factor A activity lay roughly in the region of the recently reported position of nuv, a gene controlling the production of 4-thiouridine in tRNA.
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Selected References
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- Abrell J. W., Kaufman E. E., Lipsett M. N. The biosynthesis of 4-thiouridylate. Separation and purification of two enzymes in the transfer ribonucleic acid-sulfurtransferase system. J Biol Chem. 1971 Jan 25;246(2):294–301. [PubMed] [Google Scholar]
- Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoffman E. P., Wilhelm R. C. Genetic mapping and dominance of the amber suppressor, Su1 (supD), in Escherichia coli K-12. J Bacteriol. 1970 Jul;103(1):32–36. doi: 10.1128/jb.103.1.32-36.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lipsett M. N. The isolation of 4-thiouridylic acid from the soluble ribonucleic acid of Escherichia coli. J Biol Chem. 1965 Oct;240(10):3975–3978. [PubMed] [Google Scholar]
- Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peterkofsky A., Lipsett M. N. The origin of the sulfur in s-RNA. Biochem Biophys Res Commun. 1965 Sep 22;20(6):780–786. doi: 10.1016/0006-291x(65)90086-0. [DOI] [PubMed] [Google Scholar]
- Ramabhadran T. V., Fossum T., Jagger J. Escherichia coli mutant lacking 4-thiouridine in its transfer ribonucleic acid. J Bacteriol. 1976 Nov;128(2):671–672. doi: 10.1128/jb.128.2.671-672.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas G., Favre A. Localisation génétique d'une mutation qui rend la croissance de E. coli K 12 insensible à 365 nm. C R Acad Sci Hebd Seances Acad Sci D. 1977 Jun 13;284(22):2285–2288. [PubMed] [Google Scholar]
- Thomas G., Favre A. Mutants de Escherichia coli déficients en 4-thiouridine dont la croissance est insensible à l'illumination à 365 nm. C R Acad Sci Hebd Seances Acad Sci D. 1977 Apr 4;284(14):1345–1347. [PubMed] [Google Scholar]
- Wolf R. E., Jr, Fraenkel D. G. Isolation of specialized transducing bacteriophages for gluconate 6-phosphate dehydrogenase (gnd) of Escherichia coli. J Bacteriol. 1974 Feb;117(2):468–476. doi: 10.1128/jb.117.2.468-476.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]