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. 1978 Sep;135(3):1130–1136. doi: 10.1128/jb.135.3.1130-1136.1978

Membrane phospholipid composition of Caulobacter crescentus.

I Contreras, L Shapiro, S Henry
PMCID: PMC222489  PMID: 690071

Abstract

The phospholipid composition of Caulobacter crescentus CB13 and CB15 was determined. The acidic phospholipids, phosphatidylglycerol and cardiolipin, comprise approximately 87% of the total phospholipids. Neither phosphatidylethanolamine nor its precursor phosphatidylserine was detected. The outer and inner membranes of C. crescentus CB13 were separated, and phospholipid analysis revealed heterogeneity with respect to the relative amounts of phosphatidylglycerol and cardiolipin in the two membranes. As has been shown to be the case for other bacterial membranes, the concentration of cardiolipin increases and phosphatidylglycerol decreases as cell cultures enter stationary phase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agabian N., Unger B. Caulobacter crescentus cell envelope: effect of growth conditions on murein and outer membrane protein composition. J Bacteriol. 1978 Feb;133(2):987–994. doi: 10.1128/jb.133.2.987-994.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAUMANN N. A., HAGEN P. O., GOLDFINE H. PHOSPHOLIPIDS OF CLOSTRIDIUM BUTYRICUM. STUDIES ON PLASMALOGEN COMPOSITION AND BIOSYNTHESIS. J Biol Chem. 1965 Apr;240:1559–1567. [PubMed] [Google Scholar]
  4. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  5. Braunstein S. N., Franklin R. M. Structure and synthesis of a lipid-containing bacteriophage. V. Phospholipids of the host BAL-31 and of the bacteriophage PM2. Virology. 1971 Mar;43(3):685–695. doi: 10.1016/0042-6822(71)90292-3. [DOI] [PubMed] [Google Scholar]
  6. Cronan J. E., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. doi: 10.1128/jb.95.6.2054-2061.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  8. De Siervo A. J., Salton M. R. Biosynthesis of cardiolipin in the membranes of Micrococcus lysodeikticus. Biochim Biophys Acta. 1971 Jul 13;239(2):280–292. doi: 10.1016/0005-2760(71)90174-3. [DOI] [PubMed] [Google Scholar]
  9. Diedrich D. L., Cota-Robles E. H. Heterogeneity in lipid composition of the outer membrane and cytoplasmic membrane and cytoplasmic membrane of Pseudomonas BAL-31. J Bacteriol. 1974 Sep;119(3):1006–1018. doi: 10.1128/jb.119.3.1006-1018.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  11. Houtsmuller U. M., van Deenen L. L. On the amino acid esters of phosphatidyl glycerol from bacteria. Biochim Biophys Acta. 1965 Dec 2;106(3):564–576. doi: 10.1016/0005-2760(65)90072-x. [DOI] [PubMed] [Google Scholar]
  12. Johnson R. C., Ely B. Isolation of spontaneously derived mutants of Caulobacter crescentus. Genetics. 1977 May;86(1):25–32. doi: 10.1093/genetics/86.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  14. Kurn N., Shapiro L., Agabian N. Effect of carbon source and the role of cyclic adenosine 3',5'-monophosphate on the Caulobacter cell cycle. J Bacteriol. 1977 Sep;131(3):951–959. doi: 10.1128/jb.131.3.951-959.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olsen R. W., Ballou C. E. Acyl phosphatidylglycerol. A new phospholipid from Salmonella typhimurium. J Biol Chem. 1971 May 25;246(10):3305–3313. [PubMed] [Google Scholar]
  16. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  17. Shapiro L. Differentiation in the Caulobacter cell cycle. Annu Rev Microbiol. 1976;30:377–407. doi: 10.1146/annurev.mi.30.100176.002113. [DOI] [PubMed] [Google Scholar]
  18. Shively J. M., Benson A. A. Phospholipids of Thiobacillus thiooxidans. J Bacteriol. 1967 Nov;94(5):1679–1683. doi: 10.1128/jb.94.5.1679-1683.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steiner M. R., Lester R. L. In vitro studies of phospholipid biosynthesis in Saccharomyces cerevisiae. Biochim Biophys Acta. 1972 Feb 21;260(2):222–243. doi: 10.1016/0005-2760(72)90035-5. [DOI] [PubMed] [Google Scholar]
  20. Tsukagoshi N., Kania M. N., Franklin R. M. Identification of acyl phosphatidylglycerol as a minor phospholipid of Pseudomonas BAL-31. Biochim Biophys Acta. 1976 Nov 19;450(2):131–136. doi: 10.1016/0005-2760(76)90084-9. [DOI] [PubMed] [Google Scholar]

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