Skip to main content
Molecular Medicine logoLink to Molecular Medicine
. 1999 Jan;5(1):1–8. doi: 10.1007/s008940050100

Strategies for improving responses to DNA vaccines.

J S Boyle 1, I G Barr 1, A M Lew 1
PMCID: PMC2230370  PMID: 10072442

Full text

PDF
3

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry M. A., Barry M. E., Johnston S. A. Production of monoclonal antibodies by genetic immunization. Biotechniques. 1994 Apr;16(4):616-8, 620. [PubMed] [Google Scholar]
  2. Barry M. A., Lai W. C., Johnston S. A. Protection against mycoplasma infection using expression-library immunization. Nature. 1995 Oct 19;377(6550):632–635. doi: 10.1038/377632a0. [DOI] [PubMed] [Google Scholar]
  3. Boyle J. S., Brady J. L., Koniaras C., Lew A. M. Inhibitory effect of lipopolysaccharide on immune response after DNA immunization is route dependent. DNA Cell Biol. 1998 Apr;17(4):343–348. doi: 10.1089/dna.1998.17.343. [DOI] [PubMed] [Google Scholar]
  4. Boyle J. S., Brady J. L., Lew A. M. Enhanced responses to a DNA vaccine encoding a fusion antigen that is directed to sites of immune induction. Nature. 1998 Mar 26;392(6674):408–411. doi: 10.1038/32932. [DOI] [PubMed] [Google Scholar]
  5. Boyle J. S., Koniaras C., Lew A. M. Influence of cellular location of expressed antigen on the efficacy of DNA vaccination: cytotoxic T lymphocyte and antibody responses are suboptimal when antigen is cytoplasmic after intramuscular DNA immunization. Int Immunol. 1997 Dec;9(12):1897–1906. doi: 10.1093/intimm/9.12.1897. [DOI] [PubMed] [Google Scholar]
  6. Boyle J. S., Silva A., Brady J. L., Lew A. M. DNA immunization: induction of higher avidity antibody and effect of route on T cell cytotoxicity. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14626–14631. doi: 10.1073/pnas.94.26.14626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Calarota S., Bratt G., Nordlund S., Hinkula J., Leandersson A. C., Sandström E., Wahren B. Cellular cytotoxic response induced by DNA vaccination in HIV-1-infected patients. Lancet. 1998 May 2;351(9112):1320–1325. doi: 10.1016/S0140-6736(97)09440-3. [DOI] [PubMed] [Google Scholar]
  8. Chattergoon M. A., Robinson T. M., Boyer J. D., Weiner D. B. Specific immune induction following DNA-based immunization through in vivo transfection and activation of macrophages/antigen-presenting cells. J Immunol. 1998 Jun 15;160(12):5707–5718. [PubMed] [Google Scholar]
  9. Chen Y., Hu D., Eling D. J., Robbins J., Kipps T. J. DNA vaccines encoding full-length or truncated Neu induce protective immunity against Neu-expressing mammary tumors. Cancer Res. 1998 May 1;58(9):1965–1971. [PubMed] [Google Scholar]
  10. Ciernik I. F., Berzofsky J. A., Carbone D. P. Induction of cytotoxic T lymphocytes and antitumor immunity with DNA vaccines expressing single T cell epitopes. J Immunol. 1996 Apr 1;156(7):2369–2375. [PubMed] [Google Scholar]
  11. Condon C., Watkins S. C., Celluzzi C. M., Thompson K., Falo L. D., Jr DNA-based immunization by in vivo transfection of dendritic cells. Nat Med. 1996 Oct;2(10):1122–1128. doi: 10.1038/nm1096-1122. [DOI] [PubMed] [Google Scholar]
  12. Conry R. M., Widera G., LoBuglio A. F., Fuller J. T., Moore S. E., Barlow D. L., Turner J., Yang N. S., Curiel D. T. Selected strategies to augment polynucleotide immunization. Gene Ther. 1996 Jan;3(1):67–74. [PubMed] [Google Scholar]
  13. Corr M., Lee D. J., Carson D. A., Tighe H. Gene vaccination with naked plasmid DNA: mechanism of CTL priming. J Exp Med. 1996 Oct 1;184(4):1555–1560. doi: 10.1084/jem.184.4.1555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Doe B., Selby M., Barnett S., Baenziger J., Walker C. M. Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8578–8583. doi: 10.1073/pnas.93.16.8578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Geissler M., Gesien A., Tokushige K., Wands J. R. Enhancement of cellular and humoral immune responses to hepatitis C virus core protein using DNA-based vaccines augmented with cytokine-expressing plasmids. J Immunol. 1997 Feb 1;158(3):1231–1237. [PubMed] [Google Scholar]
  16. Gerloni M., Billetta R., Xiong S., Zanetti M. Somatic transgene immunization with DNA encoding an immunoglobulin heavy chain. DNA Cell Biol. 1997 May;16(5):611–625. doi: 10.1089/dna.1997.16.611. [DOI] [PubMed] [Google Scholar]
  17. Iwasaki A., Barber B. H. Induction by DNA immunization of a protective antitumor cytotoxic T lymphocyte response against a minimal-epitope-expressing tumor. Cancer Immunol Immunother. 1998 Jan;45(5):273–279. doi: 10.1007/s002620050443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kim J. J., Ayyavoo V., Bagarazzi M. L., Chattergoon M. A., Dang K., Wang B., Boyer J. D., Weiner D. B. In vivo engineering of a cellular immune response by coadministration of IL-12 expression vector with a DNA immunogen. J Immunol. 1997 Jan 15;158(2):816–826. [PubMed] [Google Scholar]
  19. Kim J. J., Bagarazzi M. L., Trivedi N., Hu Y., Kazahaya K., Wilson D. M., Ciccarelli R., Chattergoon M. A., Dang K., Mahalingam S. Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes. Nat Biotechnol. 1997 Jul;15(7):641–646. doi: 10.1038/nbt0797-641. [DOI] [PubMed] [Google Scholar]
  20. MacGregor R. R., Boyer J. D., Ugen K. E., Lacy K. E., Gluckman S. J., Bagarazzi M. L., Chattergoon M. A., Baine Y., Higgins T. J., Ciccarelli R. B. First human trial of a DNA-based vaccine for treatment of human immunodeficiency virus type 1 infection: safety and host response. J Infect Dis. 1998 Jul;178(1):92–100. doi: 10.1086/515613. [DOI] [PubMed] [Google Scholar]
  21. Michel M. L., Davis H. L., Schleef M., Mancini M., Tiollais P., Whalen R. G. DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of the humoral response mimic hepatitis B viral infection in humans. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5307–5311. doi: 10.1073/pnas.92.12.5307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nichols W. W., Ledwith B. J., Manam S. V., Troilo P. J. Potential DNA vaccine integration into host cell genome. Ann N Y Acad Sci. 1995 Nov 27;772:30–39. doi: 10.1111/j.1749-6632.1995.tb44729.x. [DOI] [PubMed] [Google Scholar]
  23. Raz E., Carson D. A., Parker S. E., Parr T. B., Abai A. M., Aichinger G., Gromkowski S. H., Singh M., Lew D., Yankauckas M. A. Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9519–9523. doi: 10.1073/pnas.91.20.9519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Roman M., Martin-Orozco E., Goodman J. S., Nguyen M. D., Sato Y., Ronaghy A., Kornbluth R. S., Richman D. D., Carson D. A., Raz E. Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants. Nat Med. 1997 Aug;3(8):849–854. doi: 10.1038/nm0897-849. [DOI] [PubMed] [Google Scholar]
  25. Sato Y., Roman M., Tighe H., Lee D., Corr M., Nguyen M. D., Silverman G. J., Lotz M., Carson D. A., Raz E. Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science. 1996 Jul 19;273(5273):352–354. doi: 10.1126/science.273.5273.352. [DOI] [PubMed] [Google Scholar]
  26. Schneider J., Gilbert S. C., Blanchard T. J., Hanke T., Robson K. J., Hannan C. M., Becker M., Sinden R., Smith G. L., Hill A. V. Enhanced immunogenicity for CD8+ T cell induction and complete protective efficacy of malaria DNA vaccination by boosting with modified vaccinia virus Ankara. Nat Med. 1998 Apr;4(4):397–402. doi: 10.1038/nm0498-397. [DOI] [PubMed] [Google Scholar]
  27. Tang D. C., DeVit M., Johnston S. A. Genetic immunization is a simple method for eliciting an immune response. Nature. 1992 Mar 12;356(6365):152–154. doi: 10.1038/356152a0. [DOI] [PubMed] [Google Scholar]
  28. Ulivieri C., Burroni D., Telford J. L., Baldari C. T. Generation of a monoclonal antibody to a defined portion of the Helicobacter pylori vacuolating cytotoxin by DNA immunization. J Biotechnol. 1996 Nov 1;51(2):191–194. doi: 10.1016/0168-1656(96)01610-0. [DOI] [PubMed] [Google Scholar]
  29. Ulmer J. B., Deck R. R., Dewitt C. M., Donnhly J. I., Liu M. A. Generation of MHC class I-restricted cytotoxic T lymphocytes by expression of a viral protein in muscle cells: antigen presentation by non-muscle cells. Immunology. 1996 Sep;89(1):59–67. doi: 10.1046/j.1365-2567.1996.d01-718.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ulmer J. B., Donnelly J. J., Parker S. E., Rhodes G. H., Felgner P. L., Dwarki V. J., Gromkowski S. H., Deck R. R., DeWitt C. M., Friedman A. Heterologous protection against influenza by injection of DNA encoding a viral protein. Science. 1993 Mar 19;259(5102):1745–1749. doi: 10.1126/science.8456302. [DOI] [PubMed] [Google Scholar]
  31. Wang R., Doolan D. L., Le T. P., Hedstrom R. C., Coonan K. M., Charoenvit Y., Jones T. R., Hobart P., Margalith M., Ng J. Induction of antigen-specific cytotoxic T lymphocytes in humans by a malaria DNA vaccine. Science. 1998 Oct 16;282(5388):476–480. doi: 10.1126/science.282.5388.476. [DOI] [PubMed] [Google Scholar]
  32. Wolff J. A., Ludtke J. J., Acsadi G., Williams P., Jani A. Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol Genet. 1992 Sep;1(6):363–369. doi: 10.1093/hmg/1.6.363. [DOI] [PubMed] [Google Scholar]
  33. Wolff J. A., Malone R. W., Williams P., Chong W., Acsadi G., Jani A., Felgner P. L. Direct gene transfer into mouse muscle in vivo. Science. 1990 Mar 23;247(4949 Pt 1):1465–1468. doi: 10.1126/science.1690918. [DOI] [PubMed] [Google Scholar]
  34. Xiang Z. Q., Spitalnik S. L., Cheng J., Erikson J., Wojczyk B., Ertl H. C. Immune responses to nucleic acid vaccines to rabies virus. Virology. 1995 Jun 1;209(2):569–579. doi: 10.1006/viro.1995.1289. [DOI] [PubMed] [Google Scholar]
  35. Xiang Z., Ertl H. C. Manipulation of the immune response to a plasmid-encoded viral antigen by coinoculation with plasmids expressing cytokines. Immunity. 1995 Feb;2(2):129–135. doi: 10.1016/s1074-7613(95)80001-8. [DOI] [PubMed] [Google Scholar]

Articles from Molecular Medicine are provided here courtesy of The Feinstein Institute for Medical Research at North Shore LIJ

RESOURCES