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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Dec;64(4):1396–1403. doi: 10.1073/pnas.64.4.1396

NUCLEAR MAGNETIC RESONANCE STUDIES OF THE STRUCTURE AND BINDING SITES OF ENZYMES, X. PREPARATION OF SELECTIVELY DEUTERATED ANALOGS OF STAPHYLOCOCCAL NUCLEASE

I Putter 1, A Barreto 1, J L Markley 1, O Jardetzky 1
PMCID: PMC223298  PMID: 5271760

Abstract

Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The 1H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fuchs S., Cuatrecasas P., Anfinsen C. B. An improved method for the purification of staphylococcal nuclease. J Biol Chem. 1967 Oct 25;242(20):4768–4770. [PubMed] [Google Scholar]
  2. Markley J. L., Putter I., Jardetzky O. High-resolution nuclear magnetic resonance spectra of selectively deuterated staphylococcal nuclease. Science. 1968 Sep 20;161(3847):1249–1251. doi: 10.1126/science.161.3847.1249. [DOI] [PubMed] [Google Scholar]
  3. Meadows D. H., Jardetzky O., Epand R. M., Ruterjans H. H., Scheraga H. A. Assignment of the histidine peaks in the nuclear magnetic resonance spectrum of ribonuclease. Proc Natl Acad Sci U S A. 1968 Jul;60(3):766–772. doi: 10.1073/pnas.60.3.766. [DOI] [PMC free article] [PubMed] [Google Scholar]

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