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. 1969 Aug;63(4):1290–1296. doi: 10.1073/pnas.63.4.1290

GANGLIOSIDES IN DNA VIRUS-TRANSFORMED AND SPONTANEOUSLY TRANSFORMED TUMORIGENIC MOUSE CELL LINES

Peter T Mora 1,2,*, Roscoe O Brady 1,2,†, Roy M Bradley 1,2,†, Vivian W McFarland 1,2,*
PMCID: PMC223463  PMID: 4311252

Abstract

In mouse cell lines transformed by SV40 virus, a decrease was observed in the higher ganglioside homologues disialo-ceramidetetrahexoside and monosialo-ceramidetetrahexoside. Such change was observed only in cells which carry the virus genome, and it correlated with increased saturation density in tissue culture and with rejection in immunologically competent syngeneic host. This indicates that the change is one of the virus-regulated functions, and it is postulated that it relates to the rejection of the virus-transformed cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Todaro G. J. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science. 1968 Nov 29;162(3857):1024–1026. doi: 10.1126/science.162.3857.1024. [DOI] [PubMed] [Google Scholar]
  2. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  3. Gingell D., Garrod D. R. Effect of EDTA on electrophoretic mobility of slime mould cells and its relationship to current theories of cell adhesion. Nature. 1969 Jan 11;221(5176):192–193. doi: 10.1038/221192a0. [DOI] [PubMed] [Google Scholar]
  4. Hakomori S. I., Murakami W. T. Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Jan;59(1):254–261. doi: 10.1073/pnas.59.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hakomori S. I., Teather C., Andrews H. Organizational difference of cell surface "hematoside" in normal and virally transformed cells. Biochem Biophys Res Commun. 1968 Nov 25;33(4):563–568. doi: 10.1016/0006-291x(68)90332-x. [DOI] [PubMed] [Google Scholar]
  6. SVENNERHOLM E., SVENNERHOLM L. Quantitative paper partition chromatography of sialic acids. Nature. 1958 Apr 19;181(4616):1154–1155. doi: 10.1038/1811154a0. [DOI] [PubMed] [Google Scholar]
  7. SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. doi: 10.1111/j.1471-4159.1963.tb08933.x. [DOI] [PubMed] [Google Scholar]
  8. Schutz L., Mora P. T. The need for direct cell contact in "contact" inhibition of cell division in culture. J Cell Physiol. 1968 Feb;71(1):1–6. doi: 10.1002/jcp.1040710102. [DOI] [PubMed] [Google Scholar]
  9. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Takemoto K. K., Ting R. C., Ozer H. L., Fabisch P. Establishment of a cell line from an inbred mouse strain for viral transformation studies: simian virus 40 transformation and tumor production. J Natl Cancer Inst. 1968 Dec;41(6):1401–1409. [PubMed] [Google Scholar]
  11. Takemoto K. K., Todaro G. J., Habel K. Recovery of SV40 virus with genetic markers of original inducing virus from SV40-transformed mouse cells. Virology. 1968 May;35(1):1–8. doi: 10.1016/0042-6822(68)90299-7. [DOI] [PubMed] [Google Scholar]
  12. Todaro G. J., Habel K., Green H. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology. 1965 Oct;27(2):179–185. doi: 10.1016/0042-6822(65)90157-1. [DOI] [PubMed] [Google Scholar]

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