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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jul;63(3):713–717. doi: 10.1073/pnas.63.3.713

MICROELECTRODE STUDIES ON THE MEMBRANE PROPERTIES OF ISOLATED MITOCHONDRIA, II. ABSENCE OF A METABOLIC DEPENDENCE*

Joseph T Tupper 1,, Henry Tedeschi 1
PMCID: PMC223510  PMID: 4390640

Abstract

Mitochondrial membrane potentials and resistances have been determined under various metabolic conditions by the use of piezoelectric-driven microelectrodes. The mean state 3 potentials (about +19 mv) are higher than the potentials in state 4 (about +9 mv). DNP in uncoupling concentrations or KCN have no significant effect on membrane resistance or potential. Previous experiments have shown the potential to be sensitive to mitochondrial swelling and have suggested that it depends predominantly on anionic distribution.

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Selected References

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  1. Addanki S., Cahill F. D., Sotos J. F. Intramitochondrial pH and intra-extramitochondrial pH gradient of beef heart mitochondria in various functional states. Nature. 1967 Apr 22;214(5086):400–402. doi: 10.1038/214400b0. [DOI] [PubMed] [Google Scholar]
  2. Bielawski J., Thompson T. E., Lehninger A. L. The effect of 2,4-dinitrophenol on the electrical resistance of phospholipid bilayer membranes. Biochem Biophys Res Commun. 1966 Sep 22;24(6):948–954. doi: 10.1016/0006-291x(66)90342-1. [DOI] [PubMed] [Google Scholar]
  3. Chance B., Mela L. A hydrogen ion concentration gradient in a mitochondrial membrane. Nature. 1966 Oct 22;212(5060):369–372. doi: 10.1038/212369a0. [DOI] [PubMed] [Google Scholar]
  4. Chance B., Mela L. Intramitochondrial pH changes in cation accumulation. Proc Natl Acad Sci U S A. 1966 May;55(5):1243–1251. doi: 10.1073/pnas.55.5.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mitchell P. Proton-translocation phosphorylation in mitochondria, chloroplasts and bacteria: natural fuel cells and solar cells. Fed Proc. 1967 Sep;26(5):1370–1379. [PubMed] [Google Scholar]
  6. Tedeschi H., Horn H. W. 2,4 dinitrophenol and the permeability of mitochondria. Biochem Biophys Res Commun. 1967 Sep 7;28(5):752–757. doi: 10.1016/0006-291x(67)90380-4. [DOI] [PubMed] [Google Scholar]
  7. van Dam K., Tsou C. S. Accumulation of substrates by mitochondria. Biochim Biophys Acta. 1968 Oct 1;162(3):301–309. doi: 10.1016/0005-2728(68)90116-3. [DOI] [PubMed] [Google Scholar]

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