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. 1969 Jul;63(3):956–962. doi: 10.1073/pnas.63.3.956

LIGHT-INDUCED OXIDATION OF A CHLOROPLAST B-TYPE CYTOCHROME AT -189°C

David B Knaff 1, Daniel I Arnon 1,*
PMCID: PMC223545  PMID: 16591779

Abstract

The b-type cytochromes of chloroplasts have heretofore been viewed as photosynthetic electron carriers that probably occupy an intermediate position in a light-induced electron flow. The oxidation-reduction of such intermediate electron carriers, being removed from the primary photochemical reaction linked to photon capture by chlorophyll, would be expected to show a temperature dependence. Evidence has now been obtained that cytochrome b559 is photooxidized at -189°C and that this photooxidation can be induced only by “short-wavelength” monochromatic light which activates the oxygen-evolving system in chloroplasts (photosystem II). In appears, therefore, that photooxidation of cytochrome b559 is closely linked with photon capture by the chlorophyll pigments characteristic of photosystem II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnon D. I. Photosynthetic activity of isolated chloroplasts. Physiol Rev. 1967 Jul;47(3):317–358. doi: 10.1152/physrev.1967.47.3.317. [DOI] [PubMed] [Google Scholar]
  3. Arnon D. I., Tsujimoto H. Y., McSwain B. D. Ferredoxin and photosynthetic phosphorylation. Nature. 1967 May 6;214(5088):562–566. doi: 10.1038/214562a0. [DOI] [PubMed] [Google Scholar]
  4. Bendall D. S. Oxidation-reduction potentials of cytochromes in chloroplasts from higher plants. Biochem J. 1968 Sep;109(3):46P–47P. doi: 10.1042/bj1090046pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boardman N. K., Anderson J. M. Fractionation of the photochemical systems of photosynthesis. II. Cytochrome and carotenoid contents of particles isolated from spinach chloroplasts. Biochim Biophys Acta. 1967 Jul 5;143(1):187–203. doi: 10.1016/0005-2728(67)90120-x. [DOI] [PubMed] [Google Scholar]
  6. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  7. Chance B., Nishimura M. ON THE MECHANISM OF CHLOROPHYLL-CYTOCHROME INTERACTION: THE TEMPERATURE INSENSITIVITY OF LIGHT-INDUCED CYTOCHROME OXIDATION IN CHROMATIUM. Proc Natl Acad Sci U S A. 1960 Jan;46(1):19–24. doi: 10.1073/pnas.46.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cramer W. A., Butler W. L. Light-induced absorbance changes of two cytochrome b components in the electron-transport system of spinach chloroplasts. Biochim Biophys Acta. 1967 Sep 6;143(2):332–339. doi: 10.1016/0005-2728(67)90087-4. [DOI] [PubMed] [Google Scholar]
  9. DUYSENS L. N., AMESZ J., KAMP B. M. Two photochemical systems in photosynthesis. Nature. 1961 May 6;190:510–511. doi: 10.1038/190510a0. [DOI] [PubMed] [Google Scholar]
  10. HILL R. The cytochrome b component of chloroplasts. Nature. 1954 Sep 11;174(4428):501–503. doi: 10.1038/174501b0. [DOI] [PubMed] [Google Scholar]
  11. Hind G., Olson J. M. Light-induced changes in cytochrome b 6 in spinach chloroplasts. Brookhaven Symp Biol. 1966;19:188–194. [PubMed] [Google Scholar]
  12. Kalberer P. P., Buchanan B. B., Arnon D. I. Rates of photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1542–1549. doi: 10.1073/pnas.57.6.1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McSwain B. D., Arnon D. I. Enhancement effects and the identity of the two photochemical reactions of photosynthesis. Proc Natl Acad Sci U S A. 1968 Nov;61(3):989–996. doi: 10.1073/pnas.61.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RACKER E. Mechanisms of synthesis of adenosine triphosphate. Adv Enzymol Relat Subj Biochem. 1961;23:323–399. doi: 10.1002/9780470122686.ch7. [DOI] [PubMed] [Google Scholar]
  15. Rumberg B. Evidence for the participation of cytochrome b in the electron-transport system of photosynthesis. Biochim Biophys Acta. 1965 Jul 22;102(2):354–360. doi: 10.1016/0926-6585(65)90125-1. [DOI] [PubMed] [Google Scholar]
  16. TAGAWA K., TSUJIMOTO H. Y., ARNON D. I. Role of chloroplast ferredoxin in the energy conversion process of photosynthesis. Proc Natl Acad Sci U S A. 1963 Apr;49:567–572. doi: 10.1073/pnas.49.4.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TAGAWA K., TSUJIMOTO H. Y., ARNON D. I. SEPARATION BY MONOCHROMATIC LIGHT OF PHOTOSYNTHETIC PHOSPHORYLATION FROM OXYGEN EVOLUTION. Proc Natl Acad Sci U S A. 1963 Sep;50:544–549. doi: 10.1073/pnas.50.3.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WITT H. T., MUELLER A., RUMBERG B. Oxidized cytochrome and chlorophyll C2-plus in photosynthesis. Nature. 1961 Dec 9;192:967–969. doi: 10.1038/192967a0. [DOI] [PubMed] [Google Scholar]
  19. Yamashita T., Butler W. L. Photoreduction and photophosphorylation with tris-washed chloroplasts. Plant Physiol. 1968 Dec;43(12):1978–1986. doi: 10.1104/pp.43.12.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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