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. 1969 Jun;63(2):465–472. doi: 10.1073/pnas.63.2.465

ROLE OF CHROMATIN IN ESTROGEN ACTION IN THE UTERUS, II. HORMONE-INDUCED SYNTHESIS OF NONHISTONE ACIDIC PROTEINS WHICH RESTORE HISTONE-INHIBITED DNA-DEPENDENT RNA SYNTHESIS*

Ching-Sung Teng 1, Terrell H Hamilton 1
PMCID: PMC223587  PMID: 5257138

Abstract

Nonhistone acidic proteins were isolated, by equilibrium density centrifugation in 4 M cesium chloride, from the chromatin isolated and purified from the uterus of the ovariectomized rat or from calf endometrium. Evidence is presented to show (1) that arginine-rich histones are more effective inhibitors of chromatin-directed RNA synthesis in vitor than lysine-rich histones, (2) that the nonhistone acidic proteins of chromatin do not inhibit the synthesis of RNA directed by chromatin in vitro, (3) that added nonhistone acidic chromatin proteins effect a restoration of histone-inhibited RNA synthesis directed by chromatin in vitro, and (4) that the synthesis of nonhistone acidic chromatin proteins is under estrogen control in the uterus, but not in the liver. It is concluded that a major feature of the early action of estrogen in the uterus of the ovariectomized rat is the stimulation of synthesis and the accumulation in the interphase chromosomes of nonhistone acidic proteins which counter the inhibitory effect of histone on transcription by RNA polymerase. Presumably this would permit more and perhaps a new synthesis of RNA programmed for transport to the cytoplasm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLFREY V. G., LITTAU V. C., MIRSKY A. E. On the role of of histones in regulation ribonucleic acid synthesis in the cell nucleus. Proc Natl Acad Sci U S A. 1963 Mar 15;49:414–421. doi: 10.1073/pnas.49.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benjamin W., Gellhorn A. Acidic proteins of mammalian nuclei: isolation and characterization. Proc Natl Acad Sci U S A. 1968 Jan;59(1):262–268. doi: 10.1073/pnas.59.1.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berendes H. D. Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma. 1968;24(4):418–437. doi: 10.1007/BF00285017. [DOI] [PubMed] [Google Scholar]
  4. Butler J. A., Chipperfield A. R. Inhibition of RNA polymerase by histones. Nature. 1967 Sep 9;215(5106):1188–1189. doi: 10.1038/2151188a0. [DOI] [PubMed] [Google Scholar]
  5. Frenster J. H. A model of specific de-repression within interphase chromatin. Nature. 1965 Jun 19;206(990):1269–1270. doi: 10.1038/2061269a0. [DOI] [PubMed] [Google Scholar]
  6. King R. J. Fixation of steroids to receptors. Arch Anat Microsc Morphol Exp. 1967;56(3):570–583. [PubMed] [Google Scholar]
  7. Marushige K., Brutlag D., Bonner J. Properties of chromosomal nonhistone protein of rat liver. Biochemistry. 1968 Sep;7(9):3149–3155. doi: 10.1021/bi00849a018. [DOI] [PubMed] [Google Scholar]
  8. Nicolette J. A., Lemahieu M. A., Mueller G. C. A role of estrogens in the regulation of RNA polymerase in surviving rat uteri. Biochim Biophys Acta. 1968 Sep 24;166(2):403–409. doi: 10.1016/0005-2787(68)90228-1. [DOI] [PubMed] [Google Scholar]
  9. Paul J., Gilmour R. S. Organ-specific restriction of transcription in mammalian chromatin. J Mol Biol. 1968 Jul 14;34(2):305–316. doi: 10.1016/0022-2836(68)90255-6. [DOI] [PubMed] [Google Scholar]
  10. REID E. Relative importance of free alpha- and epsilon-amino groups for the biological activity of the growth hormone. Nature. 1951 Dec 1;168(4283):955–955. doi: 10.1038/168955a0. [DOI] [PubMed] [Google Scholar]
  11. Srivastava B. I. Studies on the chromatin of barley leaves during senescence. Biochem J. 1968 Dec;110(4):683–686. doi: 10.1042/bj1100683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Teng C. S., Hamilton T. H. The role of chromatin in estrogen action in the uterus, I. The control of template capacity and chemical composition and the binding of H3-estradiol-17 beta. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1410–1417. doi: 10.1073/pnas.60.4.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tidwell T., Allfrey V. G., Mirsky A. E. The methylation of histones during regeneration of the liver. J Biol Chem. 1968 Feb 25;243(4):707–715. [PubMed] [Google Scholar]
  14. Wang T. Y. Restoration of histone-inhibited DNA-dependent RNA synthesis by acidic chromatin proteins. Exp Cell Res. 1968 Oct;53(1):288–291. doi: 10.1016/0014-4827(68)90377-7. [DOI] [PubMed] [Google Scholar]

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