Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jun;63(2):504–511. doi: 10.1073/pnas.63.2.504

CYTOKININS: DISTRIBUTION IN SPECIES OF YEAST TRANSFER RNA*

D J Armstrong 1,2,3, F Skoog 1,2,3, L H Kirkegaard 1,2,3, A E Hampel 1,2,3, R M Bock 1,2,3, Ian Gillam 1,2,3, G M Tener 1,2,3
PMCID: PMC223592  PMID: 5257141

Abstract

Transfer RNA fractions from Saccharomyces lactis and Saccharomyces cerevisiae have been tested for cytokinin activity in the tobacco bioassay. Cysteine tRNA has been identified as a cytokinin-containing tRNA species in S. cerevisiae. Acid hydrolysates of S. lactis tRNA fractions (containing arginine tryptophan, and valine acceptor activities) and S. cerevisiae tRNA fractions (containing alanine, asparagine, aspartic acid, glutamic acid, glycine, histidine, tryptophan, and valine acceptor activities) were inactive in the tobacco bioassay. Cytokinins have been found only in those tRNA species corresponding to codons beginning with U.

Full text

PDF
511

Images in this article

507Image
on p.507

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellamy A. R. Cytokinins in ribonucleic acids. Nature. 1966 Sep 3;211(5053):1093–1095. doi: 10.1038/2111093a0. [DOI] [PubMed] [Google Scholar]
  2. Burrows W. J., Armstrong D. J., Skoog F., Hecht S. M., Boyle J. T., Leonard N. J., Occolowitz J. Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine. Science. 1968 Aug 16;161(3842):691–693. doi: 10.1126/science.161.3842.691. [DOI] [PubMed] [Google Scholar]
  3. Cherayil J. D., Bock R. M. A column chromatographic procedure for the fractionation of s-RNA. Biochemistry. 1965 Jun;4(6):1174–1183. doi: 10.1021/bi00882a028. [DOI] [PubMed] [Google Scholar]
  4. Fittler F., Kline L. K., Hall R. H. Biosynthesis of N6-(delta-2-isopentenyl) adenosine. The precursor relationship of acetate and mevalonate to the delta-2-isopentenyl group of the transfer ribonucleic acid of microorganisms. Biochemistry. 1968 Mar;7(3):940–944. doi: 10.1021/bi00843a009. [DOI] [PubMed] [Google Scholar]
  5. Fox J. E., Chen C. Characterization of labeled ribonucleic acid from tissue grown on 14C-containing cytokinins. J Biol Chem. 1967 Oct 10;242(19):4490–4494. [PubMed] [Google Scholar]
  6. Fox J. E. Incorporation of a kinin, N, 6-benzyladenine into soluble RNA. Plant Physiol. 1966 Jan;41(1):75–82. doi: 10.1104/pp.41.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gefter M. L., Russell R. L. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J Mol Biol. 1969 Jan 14;39(1):145–157. doi: 10.1016/0022-2836(69)90339-8. [DOI] [PubMed] [Google Scholar]
  8. Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
  9. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  10. HOLLEY R. W., APGAR J., EVERETT G. A., MADISON J. T., MARQUISEE M., MERRILL S. H., PENSWICK J. R., ZAMIR A. STRUCTURE OF A RIBONUCLEIC ACID. Science. 1965 Mar 19;147(3664):1462–1465. doi: 10.1126/science.147.3664.1462. [DOI] [PubMed] [Google Scholar]
  11. Hall R. H., Csonka L., David H., McLennan B. Cytokinins in the soluble RNA of plant tissues. Science. 1967 Apr 7;156(3771):69–71. doi: 10.1126/science.156.3771.69. [DOI] [PubMed] [Google Scholar]
  12. Harada F., Gross H. J., Kimura F., Chang S. H., Nishimura S., RajBhandary U. L. 2-Methylthio N6-(delta 2-isopentenyl) adenosine: a component of E. coli tyrosine transfer RNA. Biochem Biophys Res Commun. 1968 Oct 24;33(2):299–306. doi: 10.1016/0006-291x(68)90784-5. [DOI] [PubMed] [Google Scholar]
  13. Hecht S. M., Leonard N. J. , Occolowitz J, Burrows WJ, Armstrong DJ, Skoog F, Bock RM, Gillam I, Tener GM: Cytokinins: isolation identification of 6-(3-methyl-2-butenylamino)-9-beta-D-ribyfuranosylpurine (2iPA) from yeast cysteine tRNA. Biochem Biophys Res Commun. 1969 Apr 29;35(2):205–209. doi: 10.1016/0006-291x(69)90268-x. [DOI] [PubMed] [Google Scholar]
  14. Leonard N. J., Achmatowicz S., Loeppky R. N., Carraway K. L., Grimm W. A., Szweykowska A., Hamzi Q. H., Skoog F. Development of cytokinin activity by rearrangement of 1-substituted adenines to 6-substituted aminopurines: inactivation by N6, 1-cyclization. Proc Natl Acad Sci U S A. 1966 Aug;56(2):709–716. doi: 10.1073/pnas.56.2.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Letham D. S., Ralph R. K. A cytokinin in soluble RNA from a higher plant. Life Sci. 1967 Feb 15;6(4):387–394. doi: 10.1016/0024-3205(67)90008-2. [DOI] [PubMed] [Google Scholar]
  16. Madison J. T., Everett G. A., Kung H. K. Oligonucleoides from yeast tyrosine transfer ribonucleic acid. J Biol Chem. 1967 Mar 25;242(6):1318–1323. [PubMed] [Google Scholar]
  17. Madison J. T., Kung H. K. Large oligonucleotides isolated from yeast tyrosine transfer ribonucleic acid after partial digestion with ribonuclease T1. J Biol Chem. 1967 Mar 25;242(6):1324–1330. [PubMed] [Google Scholar]
  18. Matsubara S., Armstrong D. J., Skoog F. Cytokinins in tRNA of Corynebacterium fascians. Plant Physiol. 1968 Mar;43(3):451–453. doi: 10.1104/pp.43.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Peterkofsky A. The incorporation of mevalonic acid into the N6-(delta 2-isopentenyl) adenosine of transfer ribonucleic acid in Lactobacillus acidophilus. Biochemistry. 1968 Jan;7(1):472–482. doi: 10.1021/bi00841a059. [DOI] [PubMed] [Google Scholar]
  20. RajBhandary U. L., Faulkner R. D., Stuart A. Studies on polynucleotides. LXXIX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with pancreatic ribonuclease. J Biol Chem. 1968 Feb 10;243(3):575–583. [PubMed] [Google Scholar]
  21. Rajbhandary U. L., Chang S. H., Stuart A., Faulkner R. D., Hoskinson R. M., Khorana H. G. Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA. Proc Natl Acad Sci U S A. 1967 Mar;57(3):751–758. doi: 10.1073/pnas.57.3.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robins M. J., Hall R. H., Thedford R. N-6-(delta-3-isopentenyl) adenosine. A component of the transfer ribonucleic acid of yeast and of mammalian tissue, methods of isolation, and characterization. Biochemistry. 1967 Jun;6(6):1837–1848. doi: 10.1021/bi00858a035. [DOI] [PubMed] [Google Scholar]
  23. Skoog F., Armstrong D. J., Cherayil J. D., Hampel A. E., Bock R. M. Cytokinin activity: localization in transfer RNA preparations. Science. 1966 Dec 9;154(3754):1354–1356. doi: 10.1126/science.154.3754.1354. [DOI] [PubMed] [Google Scholar]
  24. Thiebe R., Zachau H. G. A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid. Eur J Biochem. 1968 Sep 24;5(4):546–555. doi: 10.1111/j.1432-1033.1968.tb00404.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES