Abstract
DNA after irradiation with ultraviolet light was immunogenic in rabbits and elicited serum antibodies reacting specifically with UV-irradiated DNA. The serological reactions were demonstrated by immunodiffusion, complement fixation, and immunofluorescence. By immunofluorescence, antisera reacted with cell nuclei of irradiated tissue sections but not with unirradiated tissue. This method was employed to show the presence of UV lesions in tissues of mice exposed to UV light. UV lesions in DNA were present in nuclei of epidermal cells, and in heavily irradiated animals they were also detected in the corium immediately below the epidermis. The method is useful not only for directly demonstrating UV lesions of DNA but also for localizing such lesions in tissues.
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- Cleaver J. R., Painter R. B. Evidence for repair replication of HeLa cell DNA damaged by ultraviolet light. Biochim Biophys Acta. 1968 Jul 23;161(2):552–554. doi: 10.1016/0005-2787(68)90131-7. [DOI] [PubMed] [Google Scholar]
- Evans R. G., Norman A. Radiation stimulated incorporation of thymidine into the DNA of human lymphocytes. Nature. 1968 Feb 3;217(5127):455–456. doi: 10.1038/217455a0. [DOI] [PubMed] [Google Scholar]
- Levine L., Murakami W. T., Vunakis H. V., Grossman L. SPECIFIC ANTIBODIES TO THERMALLY DENATURED DEOXYRIBONUCLEIC ACID OF PHAGE T4. Proc Natl Acad Sci U S A. 1960 Aug;46(8):1038–1043. doi: 10.1073/pnas.46.8.1038. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine L., Seaman E., Hammerschlag E., Van Vunakis H. Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light. Science. 1966 Sep 30;153(3744):1666–1667. doi: 10.1126/science.153.3744.1666. [DOI] [PubMed] [Google Scholar]
- PLESCIA O. J., BRAUN W., PALCZUK N. C. PRODUCTION OF ANTIBODIES TO DENATURED DEOXYRIBONUCLEIC ACID (DNA). Proc Natl Acad Sci U S A. 1964 Aug;52:279–285. doi: 10.1073/pnas.52.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plescia O. J., Braun W. Nucleic acids as antigens. Adv Immunol. 1967;6:231–252. doi: 10.1016/s0065-2776(08)60523-4. [DOI] [PubMed] [Google Scholar]
- RASMUSSEN R. E., PAINTER R. B. EVIDENCE FOR REPAIR OF ULTRA-VIOLET DAMAGED DEOXYRIBONUCLEIC ACID IN CULTURED MAMMALIAN CELLS. Nature. 1964 Sep 26;203:1360–1362. doi: 10.1038/2031360a0. [DOI] [PubMed] [Google Scholar]
- Setlow R. B. The photochemistry, photobiology, and repair of polynucleotides. Prog Nucleic Acid Res Mol Biol. 1968;8:257–295. doi: 10.1016/s0079-6603(08)60548-6. [DOI] [PubMed] [Google Scholar]
- Tan E. M. An immunologic precipitin system between soluble nucleoprotein and serum antibody in systemic lupus erythematosus. J Clin Invest. 1967 May;46(5):735–745. doi: 10.1172/JCI105574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tan E. M. Antibodies to deoxyribonucleic acid irradiated with ultraviolet light: detection by precipitins and immunofluorescence. Science. 1968 Sep 27;161(3848):1353–1354. doi: 10.1126/science.161.3848.1353. [DOI] [PubMed] [Google Scholar]
- Tan E. M. Relationship of nuclear staining patterns with precipitating antibodies in systemic lupus erythematosus. J Lab Clin Med. 1967 Nov;70(5):800–812. [PubMed] [Google Scholar]
- WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]
- Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]