Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Mar;62(3):793–799. doi: 10.1073/pnas.62.3.793

EVIDENCE FOR INTERGENIC COMPLEMENTATION IN HYBRID CELLS DERIVED FROM TWO HUMAN DIPLOID STRAINS EACH CARRYING AN X-LINKED MUTATION*

Marcello Siniscalco 1,2,3,†, Harold P Klinger 1,2,3,‡, Harry Eagle 1,2,3,§, Hilary Koprowski 1,2,3, Wilfred Y Fujimoto 1,2,3, J Edwin Seegmiller 1,2,3
PMCID: PMC223668  PMID: 5257005

Abstract

Two male diploid fibroblast strains, each carrying deficiency mutations at different X-linked loci (glucose-6-phosphate dehydrogenase and hypoxanthine-guanine-phosphoribosyltransferase) have been successfully hybridized. The resulting mononucleated hybrid cells have been shown to synthesize both normal gene products, indicating that both X chromosomes are functionally active in the hybrid cells. We believe this is the first reported example of intergenic complementation in fused human diploid cells.

Full text

PDF
793

Images in this article

795Image
on p.795
795Image
on p.795
797Image
on p.797
797Image
on p.797

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Danes B. S., Bearn A. G. Hurler's syndrome. A genetic study in cell culture. J Exp Med. 1966 Jan 1;123(1):1–16. doi: 10.1084/jem.123.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  3. HARRIS H., WATKINS J. F. HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES. Nature. 1965 Feb 13;205:640–646. doi: 10.1038/205640a0. [DOI] [PubMed] [Google Scholar]
  4. KROOTH R. S. PROPERTIES ODF DIPLOID CELL STRAINS DEVELOPED FROM PATIENTS WITH AN INHERITED ABNORMALITY OF URIDINE BIOSYNTHESIS. Cold Spring Harb Symp Quant Biol. 1964;29:189–212. doi: 10.1101/sqb.1964.029.01.024. [DOI] [PubMed] [Google Scholar]
  5. Knowles B. B., Jensen F. C., Steplewski Z., Koprowski H. Rescue of infectious SV40 after fusion between dfferent SV 40-transformed cells. Proc Natl Acad Sci U S A. 1968 Sep;61(1):42–45. doi: 10.1073/pnas.61.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LESCH M., NYHAN W. L. A FAMILIAL DISORDER OF URIC ACID METABOLISM AND CENTRAL NERVOUS SYSTEM FUNCTION. Am J Med. 1964 Apr;36:561–570. doi: 10.1016/0002-9343(64)90104-4. [DOI] [PubMed] [Google Scholar]
  8. STUTTS P., BROCKMAN R. W. A biochemical basis for resistance of L1210 mouse leukemia to 6-thioguanine. Biochem Pharmacol. 1963 Feb;12:97–104. doi: 10.1016/0006-2952(63)90175-8. [DOI] [PubMed] [Google Scholar]
  9. Seegmiller J. E., Rosenbloom F. M., Kelley W. N. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis. Science. 1967 Mar 31;155(3770):1682–1684. doi: 10.1126/science.155.3770.1682. [DOI] [PubMed] [Google Scholar]
  10. Wajntal A., DeMars R. A tetrazolium method for distinguishing between cultured human fibroblasts having eiter normal or deficient levels of glucose-6-phosphate dehydrogenase. Biochem Genet. 1967 Jun;1(1):61–64. doi: 10.1007/BF00487737. [DOI] [PubMed] [Google Scholar]
  11. Yoshida A. A single amino Acid substitution (asparagine to aspartic Acid) between normal (b+) and the common negro variant (a+) of human glucose-6-phosphate dehydrogenase. Proc Natl Acad Sci U S A. 1967 Mar;57(3):835–840. doi: 10.1073/pnas.57.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES