Abstract
—Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfected Escherichia coli, directed the in vitro synthesis of virus-specific glucosyl transferases.
The T4-specific α- and β-glucosyl transferases are synthesized in vivo early after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. The in vitro glucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formation in vitro, although protein synthesis was unimpaired.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Geiduschek E. P., Snyder L., Colvill A. J., Sarnat M. Selective synthesis of T-even bacteriophage early messenger in vitro. J Mol Biol. 1966 Aug;19(2):541–547. doi: 10.1016/s0022-2836(66)80021-9. [DOI] [PubMed] [Google Scholar]
- Georgopoulos C. P. Isolation and preliminary characterization of T4 mutants with nonglucosylated DNA. Biochem Biophys Res Commun. 1967 Jul 21;28(2):179–184. doi: 10.1016/0006-291x(67)90426-3. [DOI] [PubMed] [Google Scholar]
- HURWITZ J., FURTH J. J., MALAMY M., ALEXANDER M. The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1222–1230. doi: 10.1073/pnas.48.7.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOSSE J., KORNBERG A. Glucosylation of deoxyribonucleic acid. III. alpha- and beta-Glucosyl transferases from T4-infected Escherichia coli. J Biol Chem. 1962 Jun;237:1968–1976. [PubMed] [Google Scholar]
- KORNBERG S. R., ZIMMERMAN S. B., KORNBERG A. Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli. J Biol Chem. 1961 May;236:1487–1493. [PubMed] [Google Scholar]
- LEHMAN I. R., PRATT E. A. On the structure of the glucosylated hydroxymethylcytosine nucleotides of coliphages T2, T4, and T6. J Biol Chem. 1960 Nov;235:3254–3259. [PubMed] [Google Scholar]
- Lederman M., Zubay G. DNA directed peptide synthesis. V. The cell-free synthesis of a polypeptide with beta-galactosidase activity. Biochem Biophys Res Commun. 1968 Aug 21;32(4):710–714. doi: 10.1016/0006-291x(68)90297-0. [DOI] [PubMed] [Google Scholar]
- Melo A., Glaser L. Nucleotide diphosphate hexose pyrophosphatases. Biochem Biophys Res Commun. 1966 Mar 8;22(5):524–531. doi: 10.1016/0006-291x(66)90306-8. [DOI] [PubMed] [Google Scholar]
- Revel M., Gros F. A factor from E. coli required for the translation of natural messenger RNA. Biochem Biophys Res Commun. 1966 Oct 5;25(1):124–132. doi: 10.1016/0006-291x(66)90649-8. [DOI] [PubMed] [Google Scholar]
- Salser W., Gesteland R. F., Bolle A. In vitro synthesis of bacteriophage lysozyme. Nature. 1967 Aug 5;215(5101):588–591. doi: 10.1038/215588a0. [DOI] [PubMed] [Google Scholar]
- Traub P., Zillig W., Millette R. L., Schweiger M. Untersuchungen zur Biosynthese der Proteine. VII. Aktivität verschiedener Desoxyribonucleinsäuren und eines Ribonucleaseinhibitors aus Kaninchenreticulocyten in einem zellfreien Proteinsynthese-System aus Escherichia coli. DNA-abhängige in vitro-Synthese "früher Proteine" des E.-coli-Phagen T4. Hoppe Seylers Z Physiol Chem. 1965;343(4):261–275. [PubMed] [Google Scholar]
- Traub P., Zillig W. Untersuchungen zur Biosynthese der Proteine. VI. Eine neue Methode zur Darstellung eines zellfreien Systems aus Escherichia coli und deren Eigenschaften in der nucleinsäureabh-angigen Proteinsynthese. Hoppe Seylers Z Physiol Chem. 1965;343(4):246–260. [PubMed] [Google Scholar]