Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Mar;62(3):934–940. doi: 10.1073/pnas.62.3.934

ENDONUCLEASE II OF E. coli, I. ISOLATION AND PURIFICATION*

Errol C Friedberg 1,†, David A Goldthwait 1,‡
PMCID: PMC223688  PMID: 4895219

Abstract

The isolation and purification of a new endonuclease of E. coli is described. This enzyme degrades alkylated DNA as assayed by a technique that requires double-strand scission. The enzyme also makes a limited number of single-strand breaks in native nonalkylated DNA.

Full text

PDF
940

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cordonnier C., Bernardi G. Localization of E. coli endonuclease I. Biochem Biophys Res Commun. 1965 Sep 8;20(5):555–559. doi: 10.1016/0006-291x(65)90434-1. [DOI] [PubMed] [Google Scholar]
  2. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  3. Hurwitz J., Becker A., Gefter M. L., Gold M. Enzymatic reactions at termini of DNA. J Cell Physiol. 1967 Oct;70(2 Suppl):181–199. doi: 10.1002/jcp.1040700413. [DOI] [PubMed] [Google Scholar]
  4. JEFFRIES C. D., HOLTMAN D. F., GUSE D. G. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957 Apr;73(4):590–591. doi: 10.1128/jb.73.4.590-591.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LEHMAN I. R., NUSSBAUM A. L. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE). J Biol Chem. 1964 Aug;239:2628–2636. [PubMed] [Google Scholar]
  6. LEHMAN I. R., RICHARDSON C. C. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE. J Biol Chem. 1964 Jan;239:233–241. [PubMed] [Google Scholar]
  7. LEHMAN I. R., ROUSSOS G. G., PRATT E. A. The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease. J Biol Chem. 1962 Mar;237:819–828. [PubMed] [Google Scholar]
  8. Lawley P. D. Effects of some chemical mutagens and carcinogens on nucleic acids. Prog Nucleic Acid Res Mol Biol. 1966;5:89–131. doi: 10.1016/s0079-6603(08)60232-9. [DOI] [PubMed] [Google Scholar]
  9. Melgar E., Goldthwait D. A. Deoxyribonucleic acid nucleases. I. The use of a new method to observe the kinetics of deoxyribonucleic acid degradation by deoxyribonuclease I, deoxyribonuclease II, and Escherichia coli endonuclease I. J Biol Chem. 1968 Sep 10;243(17):4401–4408. [PubMed] [Google Scholar]
  10. Meselson M., Yuan R. DNA restriction enzyme from E. coli. Nature. 1968 Mar 23;217(5134):1110–1114. doi: 10.1038/2171110a0. [DOI] [PubMed] [Google Scholar]
  11. Okazaki R., Okazaki T., Sakabe K., Sugimoto K., Sugino A. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains. Proc Natl Acad Sci U S A. 1968 Feb;59(2):598–605. doi: 10.1073/pnas.59.2.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Paul A. V., Lehman I. R. The deoxyribonucleases of Escherichia coli. VII. A deoxyribonuclease induced by infection with phage T-5. J Biol Chem. 1966 Jul 25;241(14):3441–3451. [PubMed] [Google Scholar]
  13. RICHARDSON C. C., KORNBERG A. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY. J Biol Chem. 1964 Jan;239:242–250. [PubMed] [Google Scholar]
  14. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  15. Strauss B. S., Robbins M. DNA methylated in vitro by a monofunctional alkylating agent as a substrate for a specific nuclease from Micrococcus lysodeikticus. Biochim Biophys Acta. 1968 Jun 18;161(1):68–75. doi: 10.1016/0005-2787(68)90295-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES