Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1988 Jun;57(6):619–622. doi: 10.1038/bjc.1988.141

Thymidine kinase activities in mononuclear leukocytes and serum from breast cancer patients.

P G McKenna 1, K L O'Neill 1, W P Abram 1, B M Hannigan 1
PMCID: PMC2246471  PMID: 3408646

Abstract

Levels of the nucleotide pathway enzyme thymidine kinase (TK) were assayed in the mononuclear leukocytes and serum of 70 female patients with breast cancer and 98 male and 77 female non-cancer hospital patients. The total TK levels in both mononuclear leukocytes and serum from patients with breast cancer were significantly higher than in controls. The serum TK levels showed a significant correlation with cancer stage. No such correlation was observed with mononuclear leukocyte TK levels. Serum TK from 20 patients with breast cancer and 19 control patients was further assayed to ascertain the relative contributions of the thymidine kinase isozymes TK1 and TK2 to total TK levels. The increase in serum TK from breast cancer patients appears to be due to an increase in both TK1 and TK2 levels.

Full text

PDF
621

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler R., McAuslan B. R. Expression of thymidine kinase variants is a function of the replicative state of cells. Cell. 1974 Jun;2(2):113–117. doi: 10.1016/0092-8674(74)90100-7. [DOI] [PubMed] [Google Scholar]
  2. Bello L. J. Regulation of thymidine kinase synthesis in human cells. Exp Cell Res. 1974 Dec;89(2):263–274. doi: 10.1016/0014-4827(74)90790-3. [DOI] [PubMed] [Google Scholar]
  3. Caron P. C., Unsworth B. R. Alteration of the activity and molecular from of thymidine kinase during development and aging in the mouse cerebellum. Mech Ageing Dev. 1978 Sep;8(3):181–195. doi: 10.1016/0047-6374(78)90017-9. [DOI] [PubMed] [Google Scholar]
  4. Ellims P. H., Eng Gan T., Medley G., Van Der Weyden M. B. Prognostic relevance of thymidine kinase isozymes in adult non-Hodgkin's lymphoma. Blood. 1981 Nov;58(5):926–930. [PubMed] [Google Scholar]
  5. Ellims P. H., Van der Weyden M. B., Medley G. Thymidine kinase isoenzymes in human malignant lymphoma. Cancer Res. 1981 Feb;41(2):691–695. [PubMed] [Google Scholar]
  6. Gordon H. L., Bardos T. J., Chmielewicz Z. F., Ambrus J. L. Comparative study of the thymidine kinase and thymidylate kinase activities and of the feedbach inhibition of thymidine kinase in normal and neoplastic human tissue. Cancer Res. 1968 Oct;28(10):2068–2077. [PubMed] [Google Scholar]
  7. Gronowitz J. S., Hagberg H., Källander C. F., Simonsson B. The use of serum deoxythymidine kinase as a prognostic marker, and in the monitoring of patients with non-Hodgkin's lymphoma. Br J Cancer. 1983 Apr;47(4):487–495. doi: 10.1038/bjc.1983.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gronowitz J. S., Källander C. F., Hagberg H., Persson L. Deoxythymidine-kinase in cerebrospinal fluid: a new potential "marker" for brain tumours. Acta Neurochir (Wien) 1984;73(1-2):1–12. doi: 10.1007/BF01401779. [DOI] [PubMed] [Google Scholar]
  9. Hagberg H., Gronowitz S., Killander A., Källander C., Simonsson B., Sundström C., Oberg G. Serum thymidine kinase in acute leukaemia. Br J Cancer. 1984 Apr;49(4):537–540. doi: 10.1038/bjc.1984.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kit S., Leung W. C. Submitochondrial localization and characteristics of thymidine kinase molecular forms in parental and kinase-deficient HeLa cells. Biochem Genet. 1974 Mar;11(3):231–247. doi: 10.1007/BF00486058. [DOI] [PubMed] [Google Scholar]
  11. Kit S. Thymidine kinase, DNA synthesis and cancer. Mol Cell Biochem. 1976 Jun 15;11(3):161–182. doi: 10.1007/BF01744997. [DOI] [PubMed] [Google Scholar]
  12. Kreis W., Arlin Z., Yagoda A., Leyland-Jones B. R., Fiori L. Deoxycytidine and deoxythymidine kinase activities in plasma of mice and patients with neoplastic disease. Cancer Res. 1982 Jun;42(6):2514–2517. [PubMed] [Google Scholar]
  13. Källander C. F., Simonsson B., Hagberg H., Gronowitz J. S. Serum deoxythymidine kinase gives prognostic information in chronic lymphocytic leukemia. Cancer. 1984 Dec 1;54(11):2450–2455. doi: 10.1002/1097-0142(19841201)54:11<2450::aid-cncr2820541123>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  14. Machovich R., Greengard O. Thymidine kinase in rat tissues during growth and differentiation. Biochim Biophys Acta. 1972 Dec 29;286(2):375–381. doi: 10.1016/0304-4165(72)90273-5. [DOI] [PubMed] [Google Scholar]
  15. O'Neill K. L., Abram W. P., McKenna P. G. Serum thymidine kinase levels in cancer patients. Ir J Med Sci. 1986 Aug;155(8):272–274. doi: 10.1007/BF02939884. [DOI] [PubMed] [Google Scholar]
  16. O'Neill K., Abram P., Hannigan B., McKenna G. Elevated serum and mononuclear leukocyte thymidine kinase activities in patients with cancer. Ir Med J. 1987 Sep;80(9):264–265. [PubMed] [Google Scholar]
  17. Sakamoto S., Iwama T., Ebuchi M., Tsukada K., Sagara T., Kawasaki T., Murakami S., Kasahara N., Kudo H., Okamoto R. Increased activities of thymidine kinase isozymes in human mammary tumours. Br J Surg. 1986 Apr;73(4):272–273. doi: 10.1002/bjs.1800730409. [DOI] [PubMed] [Google Scholar]
  18. Simonsson B., Källander C. F., Brenning G., Killander A., Ahre A., Gronowitz J. S. Evaluation of serum deoxythymidine kinase as a marker in multiple myeloma. Br J Haematol. 1985 Oct;61(2):215–224. doi: 10.1111/j.1365-2141.1985.tb02820.x. [DOI] [PubMed] [Google Scholar]
  19. Taylor A. T., Stafford M. A., Jones O. W. Properties of thymidine kinase partially purified from human fetal and adult tissue. J Biol Chem. 1972 Mar 25;247(6):1930–1935. [PubMed] [Google Scholar]
  20. Taylor A., Jr, Jones O. W., Grishaver M. A. Effect of 5-fluorouracil on the release of thymidine kinase from hepatoma cells in vitro. Cancer Res. 1981 Jan;41(1):192–196. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES