Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1988 Mar;57(3):239–246. doi: 10.1038/bjc.1988.53

A 72 kD trophoblast glycoprotein defined by a monoclonal antibody.

N Hole 1, P L Stern 1
PMCID: PMC2246524  PMID: 3355761

Abstract

A novel trophoblast cell surface antigen has been defined by a monoclonal antibody 5T4, raised following immunisation with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM). The distribution of the antigen was determined by indirect immunoperoxidase staining of sections of normal organ and placental tissues as well as immunofluorescence and radiobinding assays with a wide variety of cell lines representing differing normal and tumour cell types. In frozen sections of normal full term placenta, 5T4 is strongly expressed only by the syncytiotrophoblast, some extravillous cytotrophoblast and the amniotic epithelium. The 5T4 antigen is apparently not expressed by any maternal component of the placenta nor is it detected in adult liver, lung, bronchus, heart, testis, ovary, brain, or muscle. The antigen is apparently expressed by several specialised epithelia. Immunoprecipitation of radiolabelled StMPM indicated that 5T4 molecules are glycoproteins of mol. wt of approximately 72 kD on SDS-PAGE. 5T4 antigen is selectively expressed by diverse tumour cell lines, including those of developmental origin. The molecular characteristics, relatively restricted normal tissue distribution and expression by certain tumour cell types make this antigen worthy of future study for use as a diagnostic marker of malignancy.

Full text

PDF
239

Images in this article

241Figure 1
on p.241
244Figure 2
on p.244
244Figure 3
on p.244

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson L. C., Nilsson K., Gahmberg C. G. K562--a human erythroleukemic cell line. Int J Cancer. 1979 Feb;23(2):143–147. doi: 10.1002/ijc.2910230202. [DOI] [PubMed] [Google Scholar]
  2. Andrews P. W., Damjanov I., Simon D., Banting G. S., Carlin C., Dracopoli N. C., Føgh J. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab Invest. 1984 Feb;50(2):147–162. [PubMed] [Google Scholar]
  3. Axelsson B., Kimura A., Hammarström S., Wigzell H., Nilsson K., Mellstedt H. Helix pomatia A hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on T cells and certain B cells. Eur J Immunol. 1978 Nov;8(11):757–764. doi: 10.1002/eji.1830081102. [DOI] [PubMed] [Google Scholar]
  4. Bramwell M. E., Ghosh A. K., Smith W. D., Wiseman G., Spriggs A. I., Harris H. Ca2 and Ca3. New monoclonal antibodies evaluated as tumor markers in serous effusions. Cancer. 1985 Jul 1;56(1):105–110. doi: 10.1002/1097-0142(19850701)56:1<105::aid-cncr2820560117>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  5. Bulmer J. N., Sunderland C. A. Bone-marrow origin of endometrial granulocytes in the early human placental bed. J Reprod Immunol. 1983 Nov;5(6):383–387. doi: 10.1016/0165-0378(83)90247-4. [DOI] [PubMed] [Google Scholar]
  6. Burchell J., Durbin H., Taylor-Papadimitriou J. Complexity of expression of antigenic determinants, recognized by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells. J Immunol. 1983 Jul;131(1):508–513. [PubMed] [Google Scholar]
  7. Critchley M., Brownless S., Patten M., McLaughlin P. J., Tromans P. M., McDicken I. W., Johnson P. M. Radionuclide imaging of epithelial ovarian tumours with 123I-labelled monoclonal antibody (H317) specific for placental-type alkaline phosphatase. Clin Radiol. 1986 Mar;37(2):107–112. doi: 10.1016/s0009-9260(86)80373-7. [DOI] [PubMed] [Google Scholar]
  8. Easty D. M., Easty G. C., Carter R. L., Monaghan P., Butler L. J. Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck. Br J Cancer. 1981 Jun;43(6):772–785. doi: 10.1038/bjc.1981.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Epenetos A. A., Snook D., Hooker G., Begent R., Durbin H., Oliver R. T., Bodmer W. F., Lavender J. P. Indium-111 labelled monoclonal antibody to placental alkaline phosphatase in in the detection of neoplasms of testis, ovary, and cervix. Lancet. 1985 Aug 17;2(8451):350–353. doi: 10.1016/s0140-6736(85)92495-x. [DOI] [PubMed] [Google Scholar]
  10. Fabricant R. N., De Larco J. E., Todaro G. J. Nerve growth factor receptors on human melanoma cells in culture. Proc Natl Acad Sci U S A. 1977 Feb;74(2):565–569. doi: 10.1073/pnas.74.2.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
  12. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surgace IgM specificity on cells derived from a Burkitt's lymphoma. Lancet. 1967 Nov 18;2(7525):1068–1070. doi: 10.1016/s0140-6736(67)90340-6. [DOI] [PubMed] [Google Scholar]
  13. Kumar S., West D., Daniel M., Hancock A., Carr T. Human lung tumour cell line adapted to grow in serum-free medium secretes angiogenesis factor. Int J Cancer. 1983 Oct 15;32(4):461–464. doi: 10.1002/ijc.2910320412. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lipinski M., Parks D. R., Rouse R. V., Herzenberg L. A. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5147–5150. doi: 10.1073/pnas.78.8.5147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Loke Y. W., Day S. Monoclonal antibody to human cytotrophoblast. Am J Reprod Immunol. 1984 Apr-May;5(3):106–108. doi: 10.1111/j.1600-0897.1984.tb00297.x. [DOI] [PubMed] [Google Scholar]
  17. MOORE A. E., SABACHEWSKY L., TOOLAN H. W. Culture characteristics of four permanent lines of human cancer cells. Cancer Res. 1955 Oct;15(9):598–602. [PubMed] [Google Scholar]
  18. McDicken I. W., McLaughlin P. J., Tromans P. M., Luesley D. M., Johnson P. M. Detection of placental-type alkaline phosphatase in ovarian cancer. Br J Cancer. 1985 Jul;52(1):59–64. doi: 10.1038/bjc.1985.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  20. Mueller U. W., Hawes C. S., Jones W. R. Cell surface antigens of human trophoblast: definition of an apparently unique system with a monoclonal antibody. Immunology. 1986 Sep;59(1):135–138. [PMC free article] [PubMed] [Google Scholar]
  21. O'Toole C. M., Povey S., Hepburn P., Franks L. M. Identity of some human bladder cancer cell lines. Nature. 1983 Feb 3;301(5899):429–430. doi: 10.1038/301429a0. [DOI] [PubMed] [Google Scholar]
  22. PULVERTAFT J. V. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA). Lancet. 1964 Feb 1;1(7327):238–240. doi: 10.1016/s0140-6736(64)92345-1. [DOI] [PubMed] [Google Scholar]
  23. Pateisky N., Philipp K., Skodler W. D., Czerwenka K., Hamilton G., Burchell J. Radioimmunodetection in patients with suspected ovarian cancer. J Nucl Med. 1985 Dec;26(12):1369–1376. [PubMed] [Google Scholar]
  24. Pattillo R. A., Gey G. O. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 1968 Jul;28(7):1231–1236. [PubMed] [Google Scholar]
  25. Plummer T. H., Jr, Elder J. H., Alexander S., Phelan A. W., Tarentino A. L. Demonstration of peptide:N-glycosidase F activity in endo-beta-N-acetylglucosaminidase F preparations. J Biol Chem. 1984 Sep 10;259(17):10700–10704. [PubMed] [Google Scholar]
  26. Rettig W. J., Cordon-Cardo C., Koulos J. P., Lewis J. L., Jr, Oettgen H. F., Old L. J. Cell surface antigens of human trophoblast and choriocarcinoma defined by monoclonal antibodies. Int J Cancer. 1985 Apr 15;35(4):469–475. doi: 10.1002/ijc.2910350409. [DOI] [PubMed] [Google Scholar]
  27. Smith N. C., Brush M. G., Luckett S. Preparation of human placental villous surface membrane. Nature. 1974 Nov 22;252(5481):302–303. doi: 10.1038/252302b0. [DOI] [PubMed] [Google Scholar]
  28. Stern P. L., Beresford N., Thompson S., Johnson P. M., Webb P. D., Hole N. Characterization of the human trophoblast-leukocyte antigenic molecules defined by a monoclonal antibody. J Immunol. 1986 Sep 1;137(5):1604–1609. [PubMed] [Google Scholar]
  29. Stern P., Gilbert P., Sternberg S., Thompson S., Chada K. A monoclonal antibody which detects a 125 kd glycoprotein on embryonal carcinoma cells and is mitogenic for murine spleen cells. J Reprod Immunol. 1984 Aug;6(5):313–328. doi: 10.1016/0165-0378(84)90030-5. [DOI] [PubMed] [Google Scholar]
  30. Sunderland C. A., Redman C. W., Stirrat G. M. Monoclonal antibodies to human syncytiotrophoblast. Immunology. 1981 Jul;43(3):541–546. [PMC free article] [PubMed] [Google Scholar]
  31. Swallow D. M., Gendler S., Griffiths B., Corney G., Taylor-Papadimitriou J., Bramwell M. E. The human tumour-associated epithelial mucins are coded by an expressed hypervariable gene locus PUM. Nature. 1987 Jul 2;328(6125):82–84. doi: 10.1038/328082a0. [DOI] [PubMed] [Google Scholar]
  32. Taylor-Papadimitriou J., Peterson J. A., Arklie J., Burchell J., Ceriani R. L., Bodmer W. F. Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer. 1981 Jul 15;28(1):17–21. doi: 10.1002/ijc.2910280104. [DOI] [PubMed] [Google Scholar]
  33. Travers P., Bodmer W. Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants. Int J Cancer. 1984 May 15;33(5):633–641. doi: 10.1002/ijc.2910330514. [DOI] [PubMed] [Google Scholar]
  34. Trowbridge I. S., Newman R. A., Domingo D. L., Sauvage C. Transferrin receptors: structure and function. Biochem Pharmacol. 1984 Mar 15;33(6):925–932. doi: 10.1016/0006-2952(84)90447-7. [DOI] [PubMed] [Google Scholar]
  35. Ullrich A., Bell J. R., Chen E. Y., Herrera R., Petruzzelli L. M., Dull T. J., Gray A., Coussens L., Liao Y. C., Tsubokawa M. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. 1985 Feb 28-Mar 6Nature. 313(6005):756–761. doi: 10.1038/313756a0. [DOI] [PubMed] [Google Scholar]
  36. Walker C., Daniel M., Mates G. Proliferative response of human venous endothelial cells to medium conditioned by human tumour cells. Cell Biol Int Rep. 1984 Sep;8(9):731–738. doi: 10.1016/0309-1651(84)90111-5. [DOI] [PubMed] [Google Scholar]
  37. Ward B. G., Cruickshank D. J. Circulating tumor-associated antigen detected by the monoclonal antibody HMFG2 in human epithelial ovarian cancer. Int J Cancer. 1987 Jan 15;39(1):30–33. doi: 10.1002/ijc.2910390107. [DOI] [PubMed] [Google Scholar]
  38. Waterfield M. D., Mayes E. L., Stroobant P., Bennet P. L., Young S., Goodfellow P. N., Banting G. S., Ozanne B. A monoclonal antibody to the human epidermal growth factor receptor. J Cell Biochem. 1982;20(2):149–161. doi: 10.1002/jcb.240200207. [DOI] [PubMed] [Google Scholar]
  39. Webb P. D., Evans P. W., Molloy C. M., Johnson P. M. Biochemical studies of human placental microvillous plasma membrane proteins. Am J Reprod Immunol Microbiol. 1985 Aug;8(4):113–119. doi: 10.1111/j.1600-0897.1985.tb00321.x. [DOI] [PubMed] [Google Scholar]
  40. Wilkinson M. J., Howell A., Harris M., Taylor-Papadimitriou J., Swindell R., Sellwood R. A. The prognostic significance of two epithelial membrane antigens expressed by human mammary carcinomas. Int J Cancer. 1984 Mar 15;33(3):299–304. doi: 10.1002/ijc.2910330304. [DOI] [PubMed] [Google Scholar]
  41. Wiseman G., Bramwell M. E., Bhavanandan V. P., Harris H. The structure of the Ca-antigen. Biochem Soc Trans. 1984 Jun;12(3):537–538. doi: 10.1042/bst0120537. [DOI] [PubMed] [Google Scholar]
  42. Yamashita K., Nakamura T., Shimizu T., Ohno H. Monoclonal antibodies to human choriocarcinoma. Am J Reprod Immunol Microbiol. 1986 Aug;11(4):130–134. doi: 10.1111/j.1600-0897.1986.tb00046.x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES