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. 1989 Feb;59(2):174–178. doi: 10.1038/bjc.1989.36

Immunolocalisation and imaging of small cell cancer xenografts by the IgG2a monoclonal antibody SWA11.

A Smith 1, R Waibel 1, G Westera 1, A Martin 1, A T Zimmerman 1, R A Stahel 1
PMCID: PMC2246986  PMID: 2539173

Abstract

We describe here a murine monoclonal antibody of the IgG2a isotype which was generated against the SW2 human small cell carcinoma cell line. The antibody, SWA11, was shown to bind to a partially defined antigen preferentially expressed on cell lines of small cell carcinoma origin. In vitro binding studies revealed 6.1 x 10(5) antigenic sites on the SW2 cell line and the Ka to be 1.2 x 10(9)M-1. Following injection into mice bearing 1-2 cm3 SW2 xenografts, SWA11 showed strong selective accumulation in the small cell heterotransplants with a tumour to blood ratio of 7.5:1 at day 4. Other tumour to organ ratios were similarly high at 19:1, 22:1 and 12:1 for liver, kidney and spleen respectively. The absolute amount of SWA11 which localised was 10.5% of total injected material per gram tumour at day 2 and this level did not markedly decrease by day 4. The high ability of SWA11 to localise to SCC xenografts was confirmed by external gamma scintigraphy. The potential application of SWA11 as a model system for in vivo radioimmunotherapy is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger C. C., Krohn K. A., Shulman H., Flournoy N., Bernstein I. D. Experimental radioimmunotherapy of murine lymphoma with 131I-labeled anti-T-cell antibodies. Cancer Res. 1986 Dec;46(12 Pt 1):6223–6228. [PubMed] [Google Scholar]
  2. Cheung N. K., Landmeier B., Neely J., Nelson A. D., Abramowsky C., Ellery S., Adams R. B., Miraldi F. Complete tumor ablation with iodine 131-radiolabeled disialoganglioside GD2-specific monoclonal antibody against human neuroblastoma xenografted in nude mice. J Natl Cancer Inst. 1986 Sep;77(3):739–745. doi: 10.1093/jnci/77.3.739. [DOI] [PubMed] [Google Scholar]
  3. Dykes P. W., Bradwell A. R., Chapman C. E., Vaughan A. T. Radioimmunotherapy of cancer: clinical studies and limiting factors. Cancer Treat Rev. 1987 Jun;14(2):87–106. doi: 10.1016/0305-7372(87)90042-9. [DOI] [PubMed] [Google Scholar]
  4. Endo K., Kamma H., Ogata T. Radiolocalization of xenografted human lung cancer with monoclonal antibody 8 in nude mice. Cancer Res. 1987 Oct 15;47(20):5427–5432. [PubMed] [Google Scholar]
  5. Epenetos A. A., Munro A. J., Stewart S., Rampling R., Lambert H. E., McKenzie C. G., Soutter P., Rahemtulla A., Hooker G., Sivolapenko G. B. Antibody-guided irradiation of advanced ovarian cancer with intraperitoneally administered radiolabeled monoclonal antibodies. J Clin Oncol. 1987 Dec;5(12):1890–1899. doi: 10.1200/JCO.1987.5.12.1890. [DOI] [PubMed] [Google Scholar]
  6. Sharkey R. M., Pykett M. J., Siegel J. A., Alger E. A., Primus F. J., Goldenberg D. M. Radioimmunotherapy of the GW-39 human colonic tumor xenograft with 131I-labeled murine monoclonal antibody to carcinoembryonic antigen. Cancer Res. 1987 Nov 1;47(21):5672–5677. [PubMed] [Google Scholar]
  7. Smedley H. M., Finan P., Lennox E. S., Ritson A., Takei F., Wraight P., Sikora K. Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody. Br J Cancer. 1983 Feb;47(2):253–259. doi: 10.1038/bjc.1983.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stahel R. A., Mabry M., Skarin A. T., Speak J., Bernal S. D. Detection of bone marrow metastasis in small-cell lung cancer by monoclonal antibody. J Clin Oncol. 1985 Apr;3(4):455–461. doi: 10.1200/JCO.1985.3.4.455. [DOI] [PubMed] [Google Scholar]
  9. Stahel R. A., O'Hara C. J., Mabry M., Waibel R., Sabbath K., Speak J. A., Bernal S. D. Cytotoxic murine monoclonal antibody LAM8 with specificity for human small cell carcinoma of the lung. Cancer Res. 1986 Apr;46(4 Pt 2):2077–2084. [PubMed] [Google Scholar]
  10. Stahel R. A., Speak J. A., Bernal S. D. Murine monoclonal antibody LAM2 defines cell membrane determinant with preferential expression on human lung small-cell carcinoma and squamous-cell carcinomas. Int J Cancer. 1985 Jan 15;35(1):11–17. doi: 10.1002/ijc.2910350104. [DOI] [PubMed] [Google Scholar]
  11. Waibel R., O'Hara C. J., Smith A., Stahel R. A. Tumor-associated membrane sialoglycoprotein on human small cell lung carcinoma identified by the IgG2a monoclonal antibody SWA20. Cancer Res. 1988 Aug 1;48(15):4318–4323. [PubMed] [Google Scholar]
  12. Waibel R., O'Hara C. J., Stahel R. A. Characterization of an epithelial and a tumor-associated human small cell lung carcinoma glycoprotein antigen. Cancer Res. 1987 Jul 15;47(14):3766–3770. [PubMed] [Google Scholar]

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