Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Jul;135(1):171–177. doi: 10.1128/jb.135.1.171-177.1978

Isolation and characterization of a plaque-forming lambda bacteriophage carrying a ColE1 plasmid.

T Mukai, H Ohkubo, K Shimada, Y Takagi
PMCID: PMC224800  PMID: 670149

Abstract

A plaque-forming lambdaimm434 bacteriophage carrying the entire genome of colicinogenic factor E1 has been isolated and characterized. This phage, lambdaimm434ColE1, can lysogenize as a stable plasmid within a recombination-deficient Escherichia coli cell that lacks the normal attachment site for lambda phage. Furthermore, it has been found that lambdaimm434ColE1 phage carrying amber mutations in the O and P genes of the lambda genome, i.e., lambdaimm434OamPamColE1, behaves as a plaque-forming phage, and this finding suggests that the ColE1 factor DNA permits replication of the DNA of the plaque-forming phage.

Full text

PDF
172

Images in this article

175Image
on p.175
176Image
on p.176

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Campbell J. L., Soll L., Richardson C. C. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090–2094. doi: 10.1073/pnas.69.8.2090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins J., Williams P., Helinski D. R. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. Mol Gen Genet. 1975;136(4):273–289. doi: 10.1007/BF00341713. [DOI] [PubMed] [Google Scholar]
  6. Fukumaki Y., Shimada K., Takagi Y. Specialized transduction of colicin E1 DNA in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3238–3242. doi: 10.1073/pnas.73.9.3238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goebel W., Schrempf H. Replication of plasmid DNA in temperature-sensitivity DNA replication mutants of Escherichia coli. Biochim Biophys Acta. 1972 Feb 23;262(1):32–41. doi: 10.1016/0005-2787(72)90216-x. [DOI] [PubMed] [Google Scholar]
  8. Ikeda H., Tomizawa J. Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968;33:791–798. doi: 10.1101/sqb.1968.033.01.091. [DOI] [PubMed] [Google Scholar]
  9. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lieb M. Lambda mutants which persist as plasmids. J Virol. 1970 Aug;6(2):218–225. doi: 10.1128/jvi.6.2.218-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maeda S., Shimada K., Takagi Y. Molecular nature of an in vitro recombinant molecule: colicin El factor carrying genes for synthesis of guanine. Biochem Biophys Res Commun. 1976 Oct 4;72(3):1129–1136. doi: 10.1016/s0006-291x(76)80249-5. [DOI] [PubMed] [Google Scholar]
  12. Matsubara K., Mukai T. Mode of replication of plasmid lambda-dv-1. J Biochem. 1975 Feb;77(2):373–382. doi: 10.1093/oxfordjournals.jbchem.a130735. [DOI] [PubMed] [Google Scholar]
  13. Matsubara K. Preparation of plasmid lambda dv from bacteriophage lambda: role of promoter-operator in the plasmid replicon. J Virol. 1974 Mar;13(3):596–602. doi: 10.1128/jvi.13.3.596-602.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matsubara K., Takagi Y., Mukai T. In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities. J Virol. 1975 Sep;16(3):479–485. doi: 10.1128/jvi.16.3.479-485.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mukai T., Matsubara K., Takagi Y. Cloning bacterial genes with plasmid gammadv. Mol Gen Genet. 1976 Aug 2;146(3):269–274. doi: 10.1007/BF00701250. [DOI] [PubMed] [Google Scholar]
  16. Ogawa T., Tomizawa J. Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions. J Mol Biol. 1968 Dec 14;38(2):217–225. doi: 10.1016/0022-2836(68)90407-5. [DOI] [PubMed] [Google Scholar]
  17. Sato K., Nishimune Y., Sato M., Numich R., Matsushiro A. Suppressor-sensitive mutants of coliphage phi-80. Virology. 1968 Apr;34(4):637–649. [PubMed] [Google Scholar]
  18. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  19. Shimada K., Fukumaki Y., Takagi Y. Expression of the guanine operon of Escherichia coli as analyzed by bacteriophage lambda induced mutations. Mol Gen Genet. 1976 Aug 19;147(2):203–208. doi: 10.1007/BF00267572. [DOI] [PubMed] [Google Scholar]
  20. Signer E. R. Plasmid formation: a new mode of lysogeny by phase lambda. Nature. 1969 Jul 12;223(5202):158–160. doi: 10.1038/223158a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES