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. 1987 Oct;99(2):523–531. doi: 10.1017/s0950268800068023

Surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on virus interference.

G Anestad
PMCID: PMC2249295  PMID: 2824225

Abstract

During the 7-year period from September 1978 to August 1985, smear specimens of nasopharyngeal secretions from 3132 patients mainly hospitalized children, taken in different regions in Norway, were examined for respiratory viruses by the rapid immunofluorescence (IF) technique. A positive diagnosis for respiratory syncytial virus (RSV), parainfluenza virus type 1, 2 and 3 or influenza A and B virus was made for 896 patients (29%). The greatest prevalence for all these viruses was observed during the colder months with only sporadic cases during the summer months. A relative increase in parainfluenza virus activity, involving several parainfluenza virus types, was observed in every second autumn and during these periods only sporadic cases of RSV infection were diagnosed. Also both RSV and parainfluenza viruses were less frequently found during influenza virus epidemics and regional differences in RSV activity were observed. During the four autumn periods 1982-85 the monthly number of positive virus identifications by IF followed an epidemic curve, while the corresponding number of negative samples was relatively constant. The results of this study suggest interference between RSV, parainfluenza viruses and influenza virus in reaching their epidemiological peaks. It is suggested that interferon might be a mediator of this effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anestad G., Breivik N., Thoresen T. Rapid diagnosis of respiratory syncytial virus and influenza A virus infections by immunofluorescence: experience with a simplified procedure for the preparation of cell smears from nasopharyngeal secretions. Acta Pathol Microbiol Immunol Scand B. 1983 Aug;91(4):267–271. doi: 10.1111/j.1699-0463.1983.tb00045.x. [DOI] [PubMed] [Google Scholar]
  2. Anestad G. Interference between outbreaks of respiratory syncytial virus and influenza virus infection. Lancet. 1982 Feb 27;1(8270):502–502. doi: 10.1016/s0140-6736(82)91466-0. [DOI] [PubMed] [Google Scholar]
  3. Anestad G., Maehle O. R. Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens. Acta Pathol Microbiol Scand B. 1981 Aug;89(4):285–287. [PubMed] [Google Scholar]
  4. Anestad G. Surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. J Hyg (Lond) 1985 Jun;94(3):349–356. doi: 10.1017/s0022172400061581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chonmaitree T., Roberts N. J., Jr, Douglas R. G., Jr, Hall C. B., Simons R. L. Interferon production by human mononuclear leukocytes: differences between respiratory syncytial virus and influenza viruses. Infect Immun. 1981 Apr;32(1):300–303. doi: 10.1128/iai.32.1.300-303.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Downham M. A., McQuillin J., Gardner P. S. Diagnosis and clinical significance of parainfluenza virus infections in children. Arch Dis Child. 1974 Jan;49(1):8–15. doi: 10.1136/adc.49.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glezen P., Denny F. W. Epidemiology of acute lower respiratory disease in children. N Engl J Med. 1973 Mar 8;288(10):498–505. doi: 10.1056/NEJM197303082881005. [DOI] [PubMed] [Google Scholar]
  8. Glezen W. P., Frank A. L., Taber L. H., Kasel J. A. Parainfluenza virus type 3: seasonality and risk of infection and reinfection in young children. J Infect Dis. 1984 Dec;150(6):851–857. doi: 10.1093/infdis/150.6.851. [DOI] [PubMed] [Google Scholar]
  9. Glezen W. P., Paredes A., Taber L. H. Influenza in children. Relationship to other respiratory agents. JAMA. 1980 Apr 4;243(13):1345–1349. doi: 10.1001/jama.243.13.1345. [DOI] [PubMed] [Google Scholar]
  10. Glezen W. P. Serious morbidity and mortality associated with influenza epidemics. Epidemiol Rev. 1982;4:25–44. doi: 10.1093/oxfordjournals.epirev.a036250. [DOI] [PubMed] [Google Scholar]
  11. Grauballe P. C., Johnsen N. J., Hornsleth A. Rapid diagnosis by immunofluorescence of viral infections associated with the croup syndrome in children. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Feb;82(1):41–47. doi: 10.1111/j.1699-0463.1974.tb02290.x. [DOI] [PubMed] [Google Scholar]
  12. Hope-Simpson R. E. Parainfluenza virus infections in the Cirencester Survey: seasonal and other characteristics. J Hyg (Lond) 1981 Dec;87(3):393–406. doi: 10.1017/s0022172400069631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyles G., Reid J. L., Chanock R. M., Parrott R. H. Epidemiology of respiratory syncytial virus infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. Am J Epidemiol. 1973 Sep;98(3):216–225. doi: 10.1093/oxfordjournals.aje.a121550. [DOI] [PubMed] [Google Scholar]
  14. Kim H. W., Brandt C. D., Arrobio J. O., Murphy B., Chanock R. M., Parrott R. H. Influenza A and B virus infection in infants and young children during the years 1957-1976. Am J Epidemiol. 1979 Apr;109(4):464–479. doi: 10.1093/oxfordjournals.aje.a112704. [DOI] [PubMed] [Google Scholar]
  15. Martin A. J., Gardner P. S., McQuillin J. Epidemiology of respiratory viral infection among paediatric inpatients over a six-year period in north-east England. Lancet. 1978 Nov 11;2(8098):1035–1038. doi: 10.1016/s0140-6736(78)92351-6. [DOI] [PubMed] [Google Scholar]
  16. McIntosh K. Interferon in nasal secretions from infants with viral respiratory tract infections. J Pediatr. 1978 Jul;93(1):33–36. doi: 10.1016/s0022-3476(78)80595-2. [DOI] [PubMed] [Google Scholar]
  17. Orstavik I., Carlsen K. H., Halvorsen K. Respiratory syncytial virus infections in Oslo 1972--1978. I. Virological and epidemiological studies. Acta Paediatr Scand. 1980 Nov;69(6):717–722. doi: 10.1111/j.1651-2227.1980.tb07139.x. [DOI] [PubMed] [Google Scholar]
  18. Orstavik I., Grandien M., Halonen P., Arstila P., Mordhorst C. H., Hornsleth A., Popow-Kraupp T., McQuillin J., Gardner P. S., Almeida J. Viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different European countries. Bull World Health Organ. 1984;62(2):307–313. [PMC free article] [PubMed] [Google Scholar]
  19. Scott G. M., Secher D. S., Flowers D., Bate J., Cantell K., Tyrrell D. A. Toxicity of interferon. Br Med J (Clin Res Ed) 1981 Apr 25;282(6273):1345–1348. doi: 10.1136/bmj.282.6273.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wright P. F., Ross K. B., Thompson J., Karzon D. T. Influenza A infections in young children. Primary natural infection and protective efficacy of live-vaccine-induced or naturally acquired immunity. N Engl J Med. 1977 Apr 14;296(15):829–834. doi: 10.1056/NEJM197704142961501. [DOI] [PubMed] [Google Scholar]

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