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. 1995 Jun;114(3):501–510. doi: 10.1017/s0950268800052213

Serosurvey and laboratory diagnosis of imported sandfly fever virus, serotype Toscana, infection in Germany.

T F Schwarz 1, G Jäger 1, S Gilch 1, C Pauli 1
PMCID: PMC2271304  PMID: 7781738

Abstract

Of eight acute infections in German tourists caused by sandfly fever virus, serotype Toscana (TOS), and diagnosed clinically and serologically, seven were acquired during visits to Tuscany, Italy, and one to Coimbra, Portugal. An indirect immunofluorescence assay (IFA) using infected cells, and a newly developed enzyme-immunoassay (EIA) using crude virus antigen prepared from infected Vero-E6 cells was used to detect anti-TOS IgM and IgG. In a seroepidemiological survey of 859 health care workers and medical students, anti-TOS IgG was detected in 1.0% by IFA, and in 0.7% by EIA. In 2034 German patients hospitalized for various diseases, 1.6% were positive for anti-TOS IgG by IFA, and 0.8% by EIA. Anti-TOS IgG was detected in 43 samples of commercial immunoglobulins at titres of 10-1000 by EIA. Although the seroprevalence of antibodies to TOS is low in Germany, TOS infection should be considered in patients returning from endemic areas who complain of fever, and headaches, and have symptoms of meningitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartelloni P. J., Tesh R. B. Clinical and serologic responses of volunteers infected with phlebotomus fever virus (Sicilian type). Am J Trop Med Hyg. 1976 May;25(3):456–462. doi: 10.4269/ajtmh.1976.25.456. [DOI] [PubMed] [Google Scholar]
  2. Calisher C. H., Weinberg A. N., Muth D. J., Lazuick J. S. Toscana virus infection in United States citizen returning from Italy. Lancet. 1987 Jan 17;1(8525):165–166. doi: 10.1016/s0140-6736(87)92005-8. [DOI] [PubMed] [Google Scholar]
  3. Corwin A., Habib M., Watts D., Darwish M., Olson J., Botros B., Hibbs R., Kleinosky M., Lee H. W., Shope R. Community-based prevalence profile of arboviral, rickettsial, and Hantaan-like viral antibody in the Nile River Delta of Egypt. Am J Trop Med Hyg. 1993 Jun;48(6):776–783. doi: 10.4269/ajtmh.1993.48.776. [DOI] [PubMed] [Google Scholar]
  4. Darwish M. A., Feinsod F. M., Scott R. M., Ksiazek T. G., Botros B. A., Farrag I. H., el Said S. Arboviral causes of non-specific fever and myalgia in a fever hospital patient population in Cairo, Egypt. Trans R Soc Trop Med Hyg. 1987;81(6):1001–1003. doi: 10.1016/0035-9203(87)90378-6. [DOI] [PubMed] [Google Scholar]
  5. Ehrnst A., Peters C. J., Niklasson B., Svedmyr A., Holmgren B. Neurovirulent Toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal. Lancet. 1985 May 25;1(8439):1212–1213. doi: 10.1016/s0140-6736(85)92886-7. [DOI] [PubMed] [Google Scholar]
  6. Eitrem R., Niklasson B., Weiland O. Sandfly fever among Swedish tourists. Scand J Infect Dis. 1991;23(4):451–457. doi: 10.3109/00365549109075093. [DOI] [PubMed] [Google Scholar]
  7. Eitrem R., Stylianou M., Niklasson B. High prevalence rates of antibody to three sandfly fever viruses (Sicilian, Naples, and Toscana) among Cypriots. Epidemiol Infect. 1991 Dec;107(3):685–691. doi: 10.1017/s0950268800049384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eitrem R., Vene S., Niklasson B. ELISA for detection of IgM and IgG antibodies to sandfly fever Sicilian virus. Res Virol. 1991 Sep-Oct;142(5):387–394. doi: 10.1016/0923-2516(91)90006-o. [DOI] [PubMed] [Google Scholar]
  9. Eitrem R., Vene S., Niklasson B. Incidence of sand fly fever among Swedish United Nations soldiers on Cyprus during 1985. Am J Trop Med Hyg. 1990 Aug;43(2):207–211. doi: 10.4269/ajtmh.1990.43.207. [DOI] [PubMed] [Google Scholar]
  10. Feinsod F. M., Ksiazek T. G., Scott R. M., Soliman A. K., Farrag I. H., Ennis W. H., Peters C. J., el Said S., Darwish M. A. Sand fly fever-Naples infection in Egypt. Am J Trop Med Hyg. 1987 Jul;37(1):193–196. doi: 10.4269/ajtmh.1987.37.193. [DOI] [PubMed] [Google Scholar]
  11. Gradoni L., Pizzuti R., di Martino L., Gramiccia M., Pempinello R., Gaeta G. B., Ferrara M., Scotti S., Altieri S. The epidemiology and surveillance of visceral leishmaniasis in the Campania region of Italy. The value of zymodeme typing. Epidemiol Infect. 1993 Oct;111(2):297–306. doi: 10.1017/s0950268800057009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nicoletti L., Verani P., Caciolli S., Ciufolini M. G., Renzi A., Bartolozzi D., Paci P., Leoncini F., Padovani P., Traini E. Central nervous system involvement during infection by Phlebovirus toscana of residents in natural foci in central Italy (1977-1988). Am J Trop Med Hyg. 1991 Oct;45(4):429–434. doi: 10.4269/ajtmh.1991.45.429. [DOI] [PubMed] [Google Scholar]
  13. Niklasson B., Eitrem R. Sandfly fever among Swedish UN troops in Cyprus. Lancet. 1985 May 25;1(8439):1212–1212. doi: 10.1016/s0140-6736(85)92885-5. [DOI] [PubMed] [Google Scholar]
  14. Schwarz T. F., Dobler G., Gilch S., Jäger G. Hepatitis C and arboviral antibodies in the island populations of Mauritius and Rodrigues. J Med Virol. 1994 Dec;44(4):379–383. doi: 10.1002/jmv.1890440411. [DOI] [PubMed] [Google Scholar]
  15. Schwarz T. F., Gilch S., Jäger G. Travel-related Toscana virus infection. Lancet. 1993 Sep 25;342(8874):803–804. doi: 10.1016/0140-6736(93)91568-7. [DOI] [PubMed] [Google Scholar]
  16. Schwarz T. F., Roggendorf M., Deinhardt F. Human parvovirus B19: ELISA and immunoblot assays. J Virol Methods. 1988 Jun;20(2):155–168. doi: 10.1016/0166-0934(88)90149-8. [DOI] [PubMed] [Google Scholar]
  17. Tesh R. B., Papaevangelou G. Effect of insecticide spraying for malaria control on the incidence of sandfly fever in Athens, Greece. Am J Trop Med Hyg. 1977 Jan;26(1):163–166. doi: 10.4269/ajtmh.1977.26.163. [DOI] [PubMed] [Google Scholar]
  18. Tesh R. B., Saidi S., Gajdamovic S. J., Rodhain F., Vesenjak-Hirjan J. Serological studies on the epidemiology of sandfly fever in the Old World. Bull World Health Organ. 1976;54(6):663–674. [PMC free article] [PubMed] [Google Scholar]
  19. Tesh R. B. The genus Phlebovirus and its vectors. Annu Rev Entomol. 1988;33:169–181. doi: 10.1146/annurev.en.33.010188.001125. [DOI] [PubMed] [Google Scholar]
  20. Tselentis Y., Gikas A., Chaniotis B. Kala-azar in Athens basin. Lancet. 1994 Jun 25;343(8913):1635–1635. doi: 10.1016/s0140-6736(94)93085-6. [DOI] [PubMed] [Google Scholar]
  21. Verani P., Nicoletti L., Ciufolini M. G. Antigenic and biological characterization of Toscana virus, a new Phlebotomus fever group virus isolated in Italy. Acta Virol. 1984 Jan;28(1):39–47. [PubMed] [Google Scholar]
  22. Vesenjak-Hirjan J., Punda-Polić V., Dobe M. Geographical distribution of arboviruses in Yugoslavia. J Hyg Epidemiol Microbiol Immunol. 1991;35(2):129–140. [PubMed] [Google Scholar]
  23. Watts D. M., MacDonald C., Bailey C. L., Meegan J. M., Peters C. J., McKee K. T., Jr Experimental infection of Phlebotomus papatasi with sand fly fever Sicilian virus. Am J Trop Med Hyg. 1988 Dec;39(6):611–616. doi: 10.4269/ajtmh.1988.39.611. [DOI] [PubMed] [Google Scholar]
  24. Wulff H., Johnson K. M. Immunoglobulin M and G responses measured by immunofluorescence in patients with Lassa or Marburg virus infections. Bull World Health Organ. 1979;57(4):631–635. [PMC free article] [PubMed] [Google Scholar]

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