Skip to main content
PMC home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1996 Aug;117(1):159–164. doi: 10.1017/s0950268800001266

Seroprevalence of astrovirus types 1 and 6 in London, determined using recombinant virus antigen.

S Kriston 1, M M Willcocks 1, M J Carter 1, W D Cubitt 1
PMCID: PMC2271669  PMID: 8760964

Abstract

We have developed a microimmunofluorescence test (IF) which uses cells infected with a recombinant baculovirus which expresses the capsid proteins of astrovirus types 1 or 6. The IF test was sensitive and specific and the results for human astrovirus type 1 (HAst-1) were comparable to those obtained by immune electronmicroscopy and radioimmunoassay. Application of the test to a panel of 273 sera collected from patients and staff at two childrens hospitals in London showed that over 50% of the population were infected by HAst-1 between the age of 5 and 12 months rising to 90% by 5 years, whereas human astrovirus type 6 (HAst6) was relatively uncommon (10-30%) in all age groups.

Full text

PDF
162

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry G. T., Heidenreich R., Kaplan P., Levine F., Mazur A., Palmieri M. J., Yudkoff M., Segal S. Branched-chain amino acid-free parenteral nutrition in the treatment of acute metabolic decompensation in patients with maple syrup urine disease. N Engl J Med. 1991 Jan 17;324(3):175–179. doi: 10.1056/NEJM199101173240307. [DOI] [PubMed] [Google Scholar]
  2. Cubitt W. D., McSwiggan D. A., Moore W. Winter vomiting disease caused by calicivirus. J Clin Pathol. 1979 Aug;32(8):786–793. doi: 10.1136/jcp.32.8.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gray J. J., Wreghitt T. G., Cubitt W. D., Elliot P. R. An outbreak of gastroenteritis in a home for the elderly associated with astrovirus type 1 and human calicivirus. J Med Virol. 1987 Dec;23(4):377–381. doi: 10.1002/jmv.1890230410. [DOI] [PubMed] [Google Scholar]
  4. Greenberg H. B., Matsui S. M. Astroviruses and caliciviruses: emerging enteric pathogens. Infect Agents Dis. 1992 Apr;1(2):71–91. [PubMed] [Google Scholar]
  5. Herrmann J. E., Cubitt W. D., Hudson R. W., Perron-Henry D. M., Oshiro L. S., Blacklow N. R. Immunological characterization of the Marin County strain of astrovirus. Arch Virol. 1990;110(3-4):213–220. doi: 10.1007/BF01311289. [DOI] [PubMed] [Google Scholar]
  6. Herrmann J. E., Hudson R. W., Blacklow N. R. Marin County agent, an astrovirus. Lancet. 1987 Sep 26;2(8561):743–743. doi: 10.1016/s0140-6736(87)91102-0. [DOI] [PubMed] [Google Scholar]
  7. Herrmann J. E., Hudson R. W., Perron-Henry D. M., Kurtz J. B., Blacklow N. R. Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis. 1988 Jul;158(1):182–185. doi: 10.1093/infdis/158.1.182. [DOI] [PubMed] [Google Scholar]
  8. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. doi: 10.1093/infdis/161.2.226. [DOI] [PubMed] [Google Scholar]
  9. Kurtz J. B., Lee T. W. Astroviruses: human and animal. Ciba Found Symp. 1987;128:92–107. doi: 10.1002/9780470513460.ch6. [DOI] [PubMed] [Google Scholar]
  10. Kurtz J., Lee T. Astrovirus gastroenteritis age distribution of antibody. Med Microbiol Immunol. 1978 Nov 17;166(1-4):227–230. doi: 10.1007/BF02121154. [DOI] [PubMed] [Google Scholar]
  11. Lee T. W., Kurtz J. B. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1994 Feb;112(1):187–193. doi: 10.1017/s0950268800057551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Midthun K., Greenberg H. B., Kurtz J. B., Gary G. W., Lin F. Y., Kapikian A. Z. Characterization and seroepidemiology of a type 5 astrovirus associated with an outbreak of gastroenteritis in Marin County, California. J Clin Microbiol. 1993 Apr;31(4):955–962. doi: 10.1128/jcm.31.4.955-962.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mitchell D. K., Monroe S. S., Jiang X., Matson D. O., Glass R. I., Pickering L. K. Virologic features of an astrovirus diarrhea outbreak in a day care center revealed by reverse transcriptase-polymerase chain reaction. J Infect Dis. 1995 Dec;172(6):1437–1444. doi: 10.1093/infdis/172.6.1437. [DOI] [PubMed] [Google Scholar]
  14. Noel J. S., Lee T. W., Kurtz J. B., Glass R. I., Monroe S. S. Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. J Clin Microbiol. 1995 Apr;33(4):797–801. doi: 10.1128/jcm.33.4.797-801.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Noel J., Cubitt D. Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981-93. Epidemiol Infect. 1994 Aug;113(1):153–159. doi: 10.1017/s0950268800051578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oishi I., Yamazaki K., Kimoto T., Minekawa Y., Utagawa E., Yamazaki S., Inouye S., Grohmann G. S., Monroe S. S., Stine S. E. A large outbreak of acute gastroenteritis associated with astrovirus among students and teachers in Osaka, Japan. J Infect Dis. 1994 Aug;170(2):439–443. doi: 10.1093/infdis/170.2.439. [DOI] [PubMed] [Google Scholar]
  17. Oshiro L. S., Haley C. E., Roberto R. R., Riggs J. L., Croughan M., Greenberg H., Kapikian A. A 27-nm virus isolated during an outbreak of acute infectious nonbacterial gastroenteritis in a convalescent hospital: a possible new serotype. J Infect Dis. 1981 Jun;143(6):791–795. doi: 10.1093/infdis/143.6.791. [DOI] [PubMed] [Google Scholar]
  18. Parker S. P., Cubitt W. D., Jiang X. J., Estes M. K. Seroprevalence studies using a recombinant Norwalk virus protein enzyme immunoassay. J Med Virol. 1994 Feb;42(2):146–150. doi: 10.1002/jmv.1890420209. [DOI] [PubMed] [Google Scholar]
  19. Utagawa E. T., Nishizawa S., Sekine S., Hayashi Y., Ishihara Y., Oishi I., Iwasaki A., Yamashita I., Miyamura K., Yamazaki S. Astrovirus as a cause of gastroenteritis in Japan. J Clin Microbiol. 1994 Aug;32(8):1841–1845. doi: 10.1128/jcm.32.8.1841-1845.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willcocks M. M., Carter M. J. Identification and sequence determination of the capsid protein gene of human astrovirus serotype 1. FEMS Microbiol Lett. 1993 Nov 15;114(1):1–7. doi: 10.1111/j.1574-6968.1993.tb06542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Willcocks M. M., Kurtz J. B., Lee T. W., Carter M. J. Prevalence of human astrovirus serotype 4: capsid protein sequence and comparison with other strains. Epidemiol Infect. 1995 Apr;114(2):385–391. doi: 10.1017/s0950268800058015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilson S. A., Cubitt W. D. The development and evaluation of radioimmune assays for the detection of immune globulins M and G against astrovirus. J Virol Methods. 1988 Feb;19(2):151–159. doi: 10.1016/0166-0934(88)90158-9. [DOI] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES