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. 1996 Oct;117(2):281–288. doi: 10.1017/s095026880000145x

Seroprevalence of brucella antibodies among persons in high-risk occupation in Lebanon.

G F Araj 1, R A Azzam 1
PMCID: PMC2271718  PMID: 8870625

Abstract

Prevalence of brucella-specific antibodies was measured in 597 persons in high-risk occupations living in 10 regions of Lebanon using the standard agglutination test (SAT), anti-human globulin (Coombs') test (AHGT) and enzyme-linked immunosorbent assay (ELISA) for measuring immunoglobulin G (IgG), IgM and IgA. The study population consisted of butchers (54%), farmers (35%), laboratory technicians (8%), abbatoir workers (2%) and veterinarians (1%), with 82% males and 18% females. The overall seroprevalence based on SAT and AHGT titres of > or = 80 was 1.7% and 15%, respectively, but seroprevalence varied by region from 0-5% in SAT and from 3.4-34% for AHGT. The overall seroprevalence based on ELISA IgG (OD > or = 0.6), IgM (OD > or = 0.6) and IgA (OD > or = 0.3) was 57, 61 and 26%, respectively. The highest seroprevalence was noted in Biqaa (34%), Kisrwan (24%), Shouf (21%), Sidon (16%) and Aley (12%) regions. Nineteen percent of those surveyed reported symptoms that could be associated with brucellosis. We conclude that exposure to brucellosis is high among persons in high-risk occupations from all surveyed regions in Lebanon. Such findings should be used to design control measures especially now that the 17 years of civil strife is over.

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Selected References

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  1. Abo-Shehada M. N., Rabi A. Z., Abuharfeil N. The prevalence of brucellosis among veterinarians in Jordan. Ann Saudi Med. 1991 May;11(3):356–357. doi: 10.5144/0256-4947.1991.356. [DOI] [PubMed] [Google Scholar]
  2. Abramson O., Rosenvasser Z., Block C., Dagan R. Detection and treatment of brucellosis by screening a population at risk. Pediatr Infect Dis J. 1991 Jun;10(6):434–438. doi: 10.1097/00006454-199106000-00003. [DOI] [PubMed] [Google Scholar]
  3. Al-Ballaa S. R., Al-Balla S. R., Al-Aska A., Kambal A., Al-Hedaithy M. A. Seasonal variation of culture positive brucellosis at a major teaching hospital. Ann Saudi Med. 1994 Jan;14(1):12–15. doi: 10.5144/0256-4947.1994.12. [DOI] [PubMed] [Google Scholar]
  4. Alausa O. K. Brucellosis: socio-economic problems and control in various countries. Trop Geogr Med. 1980 Mar;32(1):5–11. [PubMed] [Google Scholar]
  5. Araj G. F. Brucellosis in the Arabian peninsula: A continued saga that could end with optimistic rewards. Ann Saudi Med. 1993 Nov;13(6):571–572. doi: 10.5144/0256-4947.1993.571. [DOI] [PubMed] [Google Scholar]
  6. Araj G. F., Kaufmann A. F. Determination by enzyme-linked immunosorbent assay of immunoglobulin G (IgG), IgM, and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis. J Clin Microbiol. 1989 Aug;27(8):1909–1912. doi: 10.1128/jcm.27.8.1909-1912.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Araj G. F., Lulu A. R., Khateeb M. I., Saadah M. A., Shakir R. A. ELISA versus routine tests in the diagnosis of patients with systemic and neurobrucellosis. APMIS. 1988 Feb;96(2):171–176. [PubMed] [Google Scholar]
  8. Araj G. F., Lulu A. R., Mustafa M. Y., Khateeb M. I. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J Hyg (Lond) 1986 Dec;97(3):457–469. doi: 10.1017/s0022172400063634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bettelheim K. A., Maskill W. J., Pearce J. Comparison of standard tube and microagglutination techniques for determining Brucella antibodies. J Hyg (Lond) 1983 Feb;90(1):33–39. doi: 10.1017/s0022172400063804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Buchanan T. M., Sulzer C. R., Frix M. K., Feldman R. A. Brucellosis in the United States, 1960-1972. An abattoir-associated disease. Part II. Diagnostic aspects. Medicine (Baltimore) 1974 Nov;53(6):415–425. doi: 10.1097/00005792-197411000-00002. [DOI] [PubMed] [Google Scholar]
  11. Cooper C. W. Prevalence of antibody to Brucella in asymptomatic well individuals in Saudi Arabia. J Trop Med Hyg. 1992 Apr;95(2):140–142. [PubMed] [Google Scholar]
  12. Dajani Y. F., Masoud A. A., Barakat H. F. Epidemiology and diagnosis of human brucellosis in Jordan. J Trop Med Hyg. 1989 Jun;92(3):209–214. [PubMed] [Google Scholar]
  13. Gilbert G. L., Hawes L. A. The antibody response to Brucella: immunoglobulin response measured by enzyme-linked immunosorbent assay and conventional tests. Aust N Z J Med. 1981 Feb;11(1):40–45. doi: 10.1111/j.1445-5994.1981.tb03734.x. [DOI] [PubMed] [Google Scholar]
  14. Goldbaum F. A., Rubbi C. P., Wallach J. C., Miguel S. E., Baldi P. C., Fossati C. A. Differentiation between active and inactive human brucellosis by measuring antiprotein humoral immune responses. J Clin Microbiol. 1992 Mar;30(3):604–607. doi: 10.1128/jcm.30.3.604-607.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Granfors K., Viljanen M. K., Toivanen A. Measurement of immunoglobulin M, immunoglobulin G, and immunoglobulin A antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: comparison of lipopolysaccharide and whole bacterium as antigen. J Clin Microbiol. 1981 Jul;14(1):6–14. doi: 10.1128/jcm.14.1.6-14.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Heizmann W., Botzenhart K., Döller G., Schanz D., Hermann G., Fleischmann K. Brucellosis: serological methods compared. J Hyg (Lond) 1985 Dec;95(3):639–653. doi: 10.1017/s0022172400060745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hellmann E., Staak C., Baumann M. Bovine brucellosis among two different cattle populations in Bahr el Ghazal Province of Southern Sudan. Tropenmed Parasitol. 1984 Jun;35(2):123–126. [PubMed] [Google Scholar]
  18. Kiel F. W., Khan M. Y. Analysis of 506 consecutive positive serologic tests for brucellosis in Saudi Arabia. J Clin Microbiol. 1987 Aug;25(8):1384–1387. doi: 10.1128/jcm.25.8.1384-1387.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lulu A. R., Araj G. F., Khateeb M. I., Mustafa M. Y., Yusuf A. R., Fenech F. F. Human brucellosis in Kuwait: a prospective study of 400 cases. Q J Med. 1988 Jan;66(249):39–54. [PubMed] [Google Scholar]
  20. Manès G. Epidemiological situation of brucellosis in the Mediterranean countries. Dev Biol Stand. 1984;56:739–747. [PubMed] [Google Scholar]
  21. Moyer N. P., Evins G. M., Pigott N. E., Hudson J. D., Farshy C. E., Feeley J. C., Hausler W. J., Jr Comparison of serologic screening tests for brucellosis. J Clin Microbiol. 1987 Oct;25(10):1969–1972. doi: 10.1128/jcm.25.10.1969-1972.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pellicer T., Ariza J., Foz A., Pallares R., Gudiol F. Specific antibodies detected during relapse of human brucellosis. J Infect Dis. 1988 May;157(5):918–924. doi: 10.1093/infdis/157.5.918. [DOI] [PubMed] [Google Scholar]
  23. Poole P. M. A 6-year survey of human brucellosis in a rural area of north-western England and north Wales. Postgrad Med J. 1975 Jul;51(597):433–440. doi: 10.1136/pgmj.51.597.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Radwan A. I., Asmar J. A., Frerichs W. M., Bekairi S. I., Al-Mukayel A. A. Incidence of brucellosis in domestic livestock in Saudi Arabia. Trop Anim Health Prod. 1983 Aug;15(3):139–143. doi: 10.1007/BF02239921. [DOI] [PubMed] [Google Scholar]
  25. STEPHEN L. E. Brucellosis: a public health problem in Lebanon. J Med Liban. 1957 Jul;10(4):259–274. [PubMed] [Google Scholar]
  26. Seoud M., Saade G., Awar G., Uwaydah M. Brucellosis in pregnancy. J Reprod Med. 1991 Jun;36(6):441–445. [PubMed] [Google Scholar]
  27. Williams E. Brucellosis in veterinary surgeons in Wales. J R Coll Physicians Lond. 1976 Jul;10(4):351–362. [PMC free article] [PubMed] [Google Scholar]
  28. Wise R. I. Brucellosis in the United States. Past, present, and future. JAMA. 1980 Nov 21;244(20):2318–2322. [PubMed] [Google Scholar]
  29. Yagoub I. A., Mohamed A. A., Salim M. O. Serological survey of Brucella abortus antibody prevalence in the one-humped camel (Camelus dromedarius) from eastern Sudan. Rev Elev Med Vet Pays Trop. 1990;43(2):167–171. [PubMed] [Google Scholar]
  30. Young E. J. Serologic diagnosis of human brucellosis: analysis of 214 cases by agglutination tests and review of the literature. Rev Infect Dis. 1991 May-Jun;13(3):359–372. doi: 10.1093/clinids/13.3.359. [DOI] [PubMed] [Google Scholar]
  31. el Sanousi S. M., Omer E. E. Serological survey of brucellosis in Benghazi cow project (Libya). Int J Zoonoses. 1985 Sep;12(3):207–210. [PubMed] [Google Scholar]

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