Skip to main content
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1990 Feb;104(1):63–71. doi: 10.1017/s0950268800054534

Serological response of chickens to Salmonella enteritidis infection.

H Chart 1, B Rowe 1, A Baskerville 1, T J Humphrey 1
PMCID: PMC2271740  PMID: 2407544

Abstract

Fifty-eight sera, from 29 chickens originating from two layer flocks known to be naturally infected with Salmonella enteritidis phage type (PT) 4, were examined for antibodies to S. enteritidis. Using the techniques of immunoblotting and ELISA, antibodies to the lipopolysaccharide (LPS) of S. enteritidis were detected in 43 of 58 sera. Antibodies were of the IgG class and bound to the S. enteritidis LPS antigen O = 12. Bacterial agglutination reactions using whole-cell preparations of S. enteritidis and S. pullorum, correlated with anti-LPS antibody reactions as detected by immunoblotting and ELISA. A rapid means of screening chicken sera for antibodies to the LPS of S. enteritidis as an indicator of infection is discussed.

Full text

PDF
64

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chart H., Griffiths E. Antigenic and molecular homology of the ferric enterobactin receptor protein of Escherichia coli. J Gen Microbiol. 1985 Jun;131(6):1503–1509. doi: 10.1099/00221287-131-6-1503. [DOI] [PubMed] [Google Scholar]
  2. Chart H., Scotland S. M., Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol. 1989 Feb;27(2):285–290. doi: 10.1128/jcm.27.2.285-290.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chart H., Threlfall E. J., Rowe B. Virulence of Salmonella enteritidis phage type 4 is related to the possession of a 38 MDa plasmid. FEMS Microbiol Lett. 1989 Apr;49(2-3):299–303. doi: 10.1016/0378-1097(89)90057-8. [DOI] [PubMed] [Google Scholar]
  4. Coyle E. F., Palmer S. R., Ribeiro C. D., Jones H. I., Howard A. J., Ward L., Rowe B. Salmonella enteritidis phage type 4 infection: association with hen's eggs. Lancet. 1988 Dec 3;2(8623):1295–1297. doi: 10.1016/s0140-6736(88)92902-9. [DOI] [PubMed] [Google Scholar]
  5. Grannum P. A., Ghidini A., Scioscia A., Copel J. A., Romero R., Hobbins J. C. Assessment of fetal renal reserve in low level obstructive uropathy. Lancet. 1989 Feb 4;1(8632):281–282. doi: 10.1016/s0140-6736(89)91296-8. [DOI] [PubMed] [Google Scholar]
  6. Griffiths E., Stevenson P., Thorpe R., Chart H. Naturally occurring antibodies in human sera that react with the iron-regulated outer membrane proteins of Escherichia coli. Infect Immun. 1985 Mar;47(3):808–813. doi: 10.1128/iai.47.3.808-813.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hellerqvist C. G., Lindberg B., Svensson S., Holme T., Lindberg A. A. Structural studies on the O-specific side chains of the cell wall lipopolysaccharides from Salmonella typhi and S. enteritidis. Acta Chem Scand. 1969;23(5):1588–1596. doi: 10.3891/acta.chem.scand.23-1588. [DOI] [PubMed] [Google Scholar]
  8. Hopper S. A., Mawer S. Salmonella enteritidis in a commercial layer flock. Vet Rec. 1988 Sep 24;123(13):351–351. doi: 10.1136/vr.123.13.351. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakae T. Outer-membrane permeability of bacteria. Crit Rev Microbiol. 1986;13(1):1–62. doi: 10.3109/10408418609108734. [DOI] [PubMed] [Google Scholar]
  13. Thain J. A., Blandford T. B. A long-term serological study of a flock of chickens naturally infected with Salmonella pullorum. Vet Rec. 1981 Aug 15;109(7):136–138. doi: 10.1136/vr.109.7.136. [DOI] [PubMed] [Google Scholar]
  14. Thain J. A. Detection of Salmonella typhimurium infection of chickens. Vet Rec. 1978 Feb 18;102(7):143–145. doi: 10.1136/vr.102.7.143. [DOI] [PubMed] [Google Scholar]
  15. Threlfall E. J., Rowe B., Ward L. R. Subdivision of Salmonella enteritidis phage types by plasmid profile typing. Epidemiol Infect. 1989 Jun;102(3):459–465. doi: 10.1017/s095026880003017x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  17. Ward L. R., de Sa J. D., Rowe B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect. 1987 Oct;99(2):291–294. doi: 10.1017/s0950268800067765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Williams J. E., Whittemore A. D. Serological response of chickens to Salmonella thompson and Salmonella pullorum infections. J Clin Microbiol. 1979 Jan;9(1):108–114. doi: 10.1128/jcm.9.1.108-114.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES