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. 1990 Aug;105(1):107–117. doi: 10.1017/s0950268800047701

Numerical analysis of SDS-PAGE protein patterns of Serratia marcescens: a comparison with other typing methods.

B Holmes 1, M Costas 1, L L Sloss 1
PMCID: PMC2271800  PMID: 2200696

Abstract

Twenty-five cultures comprising 18 clinical isolates of Serratia marcescens from two hospitals, the type strain of S. marcescens, two reference strains of S. marinorubra, the type or a reference strain of three other Serratia species and a reference strain of undetermined species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into eight protein types. Comparison with O-serotyping indicated that the level of discrimination by SDS-PAGE was similar. As with O-serotyping, a secondary scheme, such as phage typing, is necessary to differentiate strains of the same protein type. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of S. marcescens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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