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. 1995 Sep;33(9):2473–2475. doi: 10.1128/jcm.33.9.2473-2475.1995

A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.

K E Hicks 1, S Beard 1, B J Cohen 1, J P Clewley 1
PMCID: PMC228444  PMID: 7494051

Abstract

A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given.

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Selected References

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