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. 1976 Nov;128(2):677–680. doi: 10.1128/jb.128.2.677-680.1976

Glycolytic and tricarboxylic acid cycle enzyme activities during intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli.

R B Hespell
PMCID: PMC232810  PMID: 135759

Abstract

Selected enzyme activities were measured in extracts of the total cell pellets obtained at various times during aerobic intraperiplasmic growth of Bdellovibrio bacteriovorus 109J on anaerobically grown Escherichia coli substrate cells. Initially, the glycolytic enzyme activities were associated with the input of E. coli and the tricarboxylic acid cycle enzyme activities with the input of bdellovibrios. During the first 90 min of Bdellovibrio development, the glycolytic activities declined about 25 to 60%, whereas the tricarboxylic acid cycle activities increased about 10%. Between 110 and 180 min, the glycolytic activities decreased to trace levels and tricarboxylic acid cycle activities increased about 50 to 90%. Both bdellovibrio cell extracts and the cell-free growth menstruum (obtained after bdellovibrio growth on E. coli) caused the inactivation of glycolytic enzymes in E. coli extracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carls R. A., Hanson R. S. Isolation and characterization of tricarboxylic acid cycle mutants of Bacillus subtilis. J Bacteriol. 1971 Jun;106(3):848–855. doi: 10.1128/jb.106.3.848-855.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Engelking H. M., Seidler R. J. The involvement of extracellular enzymes in the metabolism of Bdellovibrio. Arch Mikrobiol. 1974 Feb 13;95(4):293–304. doi: 10.1007/BF02451770. [DOI] [PubMed] [Google Scholar]
  3. Gloor L., Klubek B., Seidler R. J. Molecular heterogeneity of the bdellovibrios: metallo and serine proteases unique to each species. Arch Mikrobiol. 1974 Mar 1;95(1):45–56. doi: 10.1007/BF02451747. [DOI] [PubMed] [Google Scholar]
  4. Hespell R. B., Canale-Parola E. Carbohydrate metabolism in Spirochaeta stenostrepta. J Bacteriol. 1970 Jul;103(1):216–226. doi: 10.1128/jb.103.1.216-226.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hespell R. B., Miozzari G. F., Rittenberg S. C. Ribonucleic acid destruction and synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Aug;123(2):481–491. doi: 10.1128/jb.123.2.481-491.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hespell R. B., Rosson R. A., Thomashow M. F., Rittenberg S. C. Respiration of Bdellovibrio bacteriovorus strain 109J and its energy substrates for intraperiplasmic growth. J Bacteriol. 1973 Mar;113(3):1280–1288. doi: 10.1128/jb.113.3.1280-1288.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuenen J. G., Rittenberg S. C. Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth. J Bacteriol. 1975 Mar;121(3):1145–1157. doi: 10.1128/jb.121.3.1145-1157.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matin A., Rittenberg S. C. Kinetics of deoxyribonucleic acid destruction and synthesis during growth of Bdellovibrio bacteriovorus strain 109D on pseudomonas putida and escherichia coli. J Bacteriol. 1972 Sep;111(3):664–673. doi: 10.1128/jb.111.3.664-673.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rittenberg S. C., Hespell R. B. Energy efficiency of intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1158–1165. doi: 10.1128/jb.121.3.1158-1165.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rittenberg S. C., Shilo M. Early host damage in the infection cycle of Bdellovibrio bacteriovorus. J Bacteriol. 1970 Apr;102(1):149–160. doi: 10.1128/jb.102.1.149-160.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Seideler R. J., Mandel M., Baptist J. N. Molecular heterogeneity of the Bdellovibrios: evidence of two new species. J Bacteriol. 1972 Jan;109(1):209–217. doi: 10.1128/jb.109.1.209-217.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seidler R. J., Starr M. P. Isolation and characterization of host-independent Bdellovibrios. J Bacteriol. 1969 Nov;100(2):769–785. doi: 10.1128/jb.100.2.769-785.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shilo M., Bruff B. Lysis of Gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates. J Gen Microbiol. 1965 Sep;40(3):317–328. doi: 10.1099/00221287-40-3-317. [DOI] [PubMed] [Google Scholar]
  14. Simpson F. J., Robinson J. Some energy-producing systems in Bdellovibrio bacteriovorus, strain 6-5-S. Can J Biochem. 1968 Aug;46(8):865–873. doi: 10.1139/o68-129. [DOI] [PubMed] [Google Scholar]
  15. Varon M., Drucker I., Shilo M. Early effects of Bdellovibrio infection on the syntheses of protein and RNA of host bacteria. Biochem Biophys Res Commun. 1969 Oct 22;37(3):518–525. doi: 10.1016/0006-291x(69)90946-2. [DOI] [PubMed] [Google Scholar]

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