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. 1976 Sep;127(3):1286–1291. doi: 10.1128/jb.127.3.1286-1291.1976

Membrane-bound respiratory of Spirillum itersonii.

H A Dailey Jr
PMCID: PMC232922  PMID: 182674

Abstract

The membrane-bound respiratory system of the gram-negative bacterium Spirillum itersonii was investigated. It contains cytochromes b (558), c (550), and o (558) and beta-dihydro-nicotinamide adenine dinucleotide (NADH) and succinate oxidase activities under all growth conditions. It is also capable of producing D-lactate and alpha-glycerophosphate dehydrogenases when grown with lactate or glycerol as sole carbon source. Membrane-bound malate dehydrogenase was not detectable under any conditions, although there is high activity of soluble nicotinamide adenine dinucleotide: malate dehydrogenase. When grown with oxygen as the sole terminal electron acceptor, approximately 60% of the total b-type cytochrome is present as cytochrome o, whereas only 40% is present as cytochrome o in cells grown with nitrate in the presence of oxygen. Both NADH and succinate oxidase are inhibited by azide, cyanide, antimycin A, and 2-n-heptyl-4-hydroxyquinoline-N-oxidase at low concentrations. The ability of these inhibitors to completely inhibit oxidase activity at low concentrations and their effects upon the aerobic steady-state reduction levels of b- and c-type cytochromes as well as the aerobic steady-state reduction levels obtained with NADH, succinate, and ascorbate-dichlorophenolindophenol suggest that presence of an unbranched respiratory chain in S. itersonii with the order ubiquinone leads to b leads to c leads to c leads to oxygen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broberg P. L., Smith L. The cytochrome system of Bacillus megaterium KM. The presence and some properties of two CO-binding cytochromes. Biochim Biophys Acta. 1967 May 9;131(3):479–489. doi: 10.1016/0005-2728(67)90007-2. [DOI] [PubMed] [Google Scholar]
  2. Clark-Walker G. D., Lascelles J. Cytochrome c550 from Spirillum itersonii: purification and some properties. Arch Biochem Biophys. 1970 Jan;136(1):153–159. doi: 10.1016/0003-9861(70)90336-x. [DOI] [PubMed] [Google Scholar]
  3. Clark-Walker G. D., Rittenberg B., Lascelles J. Cytochrome synthesis and its regulation in Spirillum itersonii. J Bacteriol. 1967 Nov;94(5):1648–1655. doi: 10.1128/jb.94.5.1648-1655.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cole J. A., Rittenberg S. C. A comparison of respiratory processes in Spirillum volutans, Spirillum itersonii and Spirillum serpens. J Gen Microbiol. 1971 Dec;69(3):375–383. doi: 10.1099/00221287-69-3-375. [DOI] [PubMed] [Google Scholar]
  5. Dailey H. A., Jr, Lascelles J. Ferrochelatase activity in wild-type and mutant strains of Spirillum itersonii. Solubilization with chaotropic reagents. Arch Biochem Biophys. 1974 Feb;160(2):523–529. doi: 10.1016/0003-9861(74)90429-9. [DOI] [PubMed] [Google Scholar]
  6. Daniel R. M. The electron transport system of Acetobacter suboxydans with particular reference to cytochrome. Biochim Biophys Acta. 1970 Sep 1;216(2):328–341. doi: 10.1016/0005-2728(70)90224-0. [DOI] [PubMed] [Google Scholar]
  7. Erickson S. K. The respiratory system of the aerobic, nitrogen-fixing, gram-positive bacterium, Mycobacterium flavum 301. Biochim Biophys Acta. 1971 Aug 6;245(1):63–69. doi: 10.1016/0005-2728(71)90008-9. [DOI] [PubMed] [Google Scholar]
  8. Garrard W. T. Synthesis, assembly, and localization of periplasmic cytochrome c. J Biol Chem. 1972 Sep 25;247(18):5935–5943. [PubMed] [Google Scholar]
  9. Gauthier D. K., Clark-Walker G. D., Garrard W. T., Jr, Lascelles J. Nitrate reductase and soluble cytochrome c in Spirillum itersonii. J Bacteriol. 1970 Jun;102(3):790–801. doi: 10.1128/jb.102.3.797-803.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ho Y. K., Lascelles J. -aminolevulinic acid dehydratase of Spirillum itersonii and the regulation of tetrapyrrole synthesis. Arch Biochem Biophys. 1971 Jun;144(2):734–740. doi: 10.1016/0003-9861(71)90381-x. [DOI] [PubMed] [Google Scholar]
  11. Jones C. W., Redfearn E. R. The cytochrome system of Azotobacter vinelandii. Biochim Biophys Acta. 1967 Sep 6;143(2):340–353. doi: 10.1016/0005-2728(67)90088-6. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. PORRA R. J., LASCELLES J. HAEMOPROTEINS AND HAEM SYNTHESIS IN FACULTATIVE PHOTOSYNTHETIC AND DENITRIFYING BACTERIA. Biochem J. 1965 Jan;94:120–126. doi: 10.1042/bj0940120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PUMPHREY A. M., REDFEARN E. R. A method for determining the concentration of ubiquinone in mitochondrial preparations. Biochem J. 1960 Jul;76:61–64. doi: 10.1042/bj0760061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sapshead L. M., Wimpenny J. W. The influence of oxygen and nitrate on the formation of the cytochrome pigments of the aerobic and anaerobic respiratory chain of Micrococcus denitrificans. Biochim Biophys Acta. 1972 May 25;267(2):388–397. doi: 10.1016/0005-2728(72)90126-0. [DOI] [PubMed] [Google Scholar]
  16. Scholes P. B., Smith L. Composition and properties of the membrane-bound respiratory chain system of Micrococcus denitrificans. Biochim Biophys Acta. 1968 Feb 12;153(2):363–375. doi: 10.1016/0005-2728(68)90081-9. [DOI] [PubMed] [Google Scholar]
  17. Sinclair P. R., White D. C. Effect of nitrate, fumarate, and oxygen on the formation of the membrane-bound electron transport system of Haemophilus parainfluenzae. J Bacteriol. 1970 Feb;101(2):365–372. doi: 10.1128/jb.101.2.365-372.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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