Abstract
The rate of synthesis of ribosomal RNA (rRNA) is much slower during sporulation than during vegetative growth of yeast. If sporulating cells are transferred from normal incubation conditions at pH 8.8 to the same medium adjusted to pH 7.0, the rate of rRNA synthesis increased to approach that observed in vegetative cells. The response to the pH change is quite rapid, occurring within 10 min. THE PH-dependent, rate-limiting step appears to be in the processing of 35S ribosomal precursor RNA to the final 26S and 18S RNA species. A similar pH effect also was found for the rate of protein synthesis. However, no change in respiration was observed when the pH was lowered. These results indicate that the observed differences in rate of rRNA synthesis in vegetative and sporulating cells are a consequence of pH and are not intrinsic to sporulation. The results also support the correlation between rRNA processing and protein synthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chaffin W. L., Sogin S. J., Halvorson H. O. Nature of ribonucleic acid synthesis during early sporulation in Saccharomyces cerevisiae. J Bacteriol. 1974 Nov;120(2):872–879. doi: 10.1128/jb.120.2.872-879.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopper A. K., Magee P. T., Welch S. K., Friedman M., Hall B. D. Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast. J Bacteriol. 1974 Aug;119(2):619–628. doi: 10.1128/jb.119.2.619-628.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mills D. Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae. J Bacteriol. 1972 Oct;112(1):519–526. doi: 10.1128/jb.112.1.519-526.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAHL A. J., BAKES J., WEIL J. H., EBEL J. P. ETUDE DU TRANSFERT DU PHOSPHORE DES POLYPHOSPHATES INORGANIQUES DANS LES ACIDES RIBONUCH'EIQUES CHEZ LA LEVURE. II. DISTRIBUTION DU PHOSPHORE POLYPHOSPHORIQUE DANS LES DIVERSES FRACTIONS RIBONUCL'EIQUES. Bull Soc Chim Biol (Paris) 1964;46:1017–1026. [PubMed] [Google Scholar]
- Sogin S. J., Haber J. E., Halvorson H. O. Relationship between sporulation-specific 20S ribonucleic acid and ribosomal ribonucleic acid processing in Saccharomyces cerevisiae. J Bacteriol. 1972 Nov;112(2):806–814. doi: 10.1128/jb.112.2.806-814.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turco E., Altruda F., Ponzetto A., Mangiarotti G. Ribosome biosynthesis in Escherichia coli. Concerning the limiting step. Biochemistry. 1974 Nov 5;13(23):4752–4757. doi: 10.1021/bi00720a011. [DOI] [PubMed] [Google Scholar]
- Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
- Wejksnora P. J., Haber J. E. Methionine-dependent synthesis of ribosomal ribonucleic acid during sporulation and vegetative growth of Saccharomyces cerevisiae. J Bacteriol. 1974 Dec;120(3):1344–1355. doi: 10.1128/jb.120.3.1344-1355.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]