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. 1976 Jun;126(3):1108–1112. doi: 10.1128/jb.126.3.1108-1112.1976

Suppression of polarity of insertion mutations in the gal operon and N mutations in bacteriophage lambda.

O Reyes, M Gottesman, S Adhya
PMCID: PMC233132  PMID: 181361

Abstract

Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The short insertion, IS1 (800 nucleotide pairs), is about 15 to 50% suppressed, whereas longer insertions, IS2 (1,400 nucleotide pairs), and IS3 (1,200 nucleotide pairs), are not. Some of the polarity suppressor mutations (psu-1, psu-2, and psu-3) are at least partially permissive for N-gene mutations (N7 and N53) of bacteriophage lambda, suggesting a relationship between natural and mutational polar signals. That this relationship may be complex is indicated by the fact that other suppressor mutations, effective in suppressing nonsense or insertion polarity, fail entirely to permit the growth of lambda N mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S. L., Shapiro J. A. The galactose operon of E. coli K-12. I. Structural and pleiotropic mutations of the operon. Genetics. 1969 Jun;62(2):231–247. doi: 10.1093/genetics/62.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adhya S., Gottesman M., De Crombrugghe B. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534–2538. doi: 10.1073/pnas.71.6.2534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adhya S., Schwartz M. Phosphoglucomutase mutants of Escherichia coli K-12. J Bacteriol. 1971 Nov;108(2):621–626. doi: 10.1128/jb.108.2.621-626.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ahmed A., Scraba D. The nature of the gal3 mutation of Escherichia coli. Mol Gen Genet. 1975;136(3):233–242. doi: 10.1007/BF00334018. [DOI] [PubMed] [Google Scholar]
  5. BECKWITH J. RESTORATION OF OPERON ACTIVITY BY SUPPRESSORS. Biochim Biophys Acta. 1963 Sep 17;76:162–164. [PubMed] [Google Scholar]
  6. Brunel F., Davison J. Bacterial mutants able to partly suppress the effect of N mutations in bacteriophage lambda. Mol Gen Genet. 1975;136(2):167–180. doi: 10.1007/BF00272037. [DOI] [PubMed] [Google Scholar]
  7. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  8. Carter T., Newton A. New polarity suppressors in Escherichia coli: suppression and messenger RNA stability. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2962–2966. doi: 10.1073/pnas.68.12.2962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. De Crombrugghe B., Adhya S., Gottesman M., Pastan I. Effect of Rho on transcription of bacterial operons. Nat New Biol. 1973 Feb 28;241(113):260–264. doi: 10.1038/newbio241260a0. [DOI] [PubMed] [Google Scholar]
  10. Fiandt M., Szybalski W., Malamy M. H. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation. Mol Gen Genet. 1972;119(3):223–231. doi: 10.1007/BF00333860. [DOI] [PubMed] [Google Scholar]
  11. Franklin N. C. Altered reading of genetic signals fused to the N operon of bacteriophage lambda: genetic evidence for modification of polymerase by the protein product of the N gene. J Mol Biol. 1974 Oct 15;89(1):33–48. doi: 10.1016/0022-2836(74)90161-2. [DOI] [PubMed] [Google Scholar]
  12. Hirsch H. J., Starlinger P., Brachet P. Two kinds of insertions in bacterial genes. Mol Gen Genet. 1972;119(3):191–206. doi: 10.1007/BF00333858. [DOI] [PubMed] [Google Scholar]
  13. Malamy M. H., Fiandt M., Szybalski W. Electron microscopy of polar insertions in the lac operon of Escherichia coli. Mol Gen Genet. 1972;119(3):207–222. doi: 10.1007/BF00333859. [DOI] [PubMed] [Google Scholar]
  14. Morse D. E., Guertin M. Amber suA mutations which relieve polarity. J Mol Biol. 1972 Feb 14;63(3):605–608. doi: 10.1016/0022-2836(72)90453-6. [DOI] [PubMed] [Google Scholar]
  15. Ratner D. Evidence that mutations in the suA polarity suppressing gene directly affect termination factor rho. Nature. 1976 Jan 15;259(5539):151–153. doi: 10.1038/259151a0. [DOI] [PubMed] [Google Scholar]
  16. Richardson J. P., Grimley C., Lowery C. Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations. Proc Natl Acad Sci U S A. 1975 May;72(5):1725–1728. doi: 10.1073/pnas.72.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  18. Saedler H., Besemer J., Kemper B., Rosenwirth B., Starlinger P. Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations. Mol Gen Genet. 1972;115(3):258–265. doi: 10.1007/BF00268889. [DOI] [PubMed] [Google Scholar]
  19. Thomas R., Leurs C., Dambly C., Parmentier D., Lambert L., Brachet P., Lefebvre N., Mousset S., Porcheret J., Szpirer J. Isolation and characterization of new sus (amber) mutants of bacteriophage lambda. Mutat Res. 1967 Nov-Dec;4(6):735–741. doi: 10.1016/0027-5107(67)90082-6. [DOI] [PubMed] [Google Scholar]

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