Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Jun;126(3):1245–1249. doi: 10.1128/jb.126.3.1245-1249.1976

Role of L-threonine dehydrogenase in the catabolism of threonine and synthesis of glycine by Escherichia coli.

E B Newman, V Kapoor, R Potter
PMCID: PMC233149  PMID: 7548

Abstract

The enzyme L-threonine dehydrogenase was demonstrated in extracts of Escherichia coli K-12, and was shown to be the first enzyme of the pathway converting threonine to glycine. The enzyme was induced by L-leucine, but not by its substrate, L-threonine. The metabolic significance of leucine as a catabolic signal for amino acid degradation is considered.

Full text

PDF
1246

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackmore M. A., Turner J. M. Threonine metabolism via two-carbon compounds by Pseudomonas oxalaticus. J Gen Microbiol. 1971 Aug;67(2):243–246. doi: 10.1099/00221287-67-2-243. [DOI] [PubMed] [Google Scholar]
  2. Deutch C. E., Soffer R. L. Regulation of proline catabolism by leucyl,phenylalanyl-tRNA-protein transferase. Proc Natl Acad Sci U S A. 1975 Jan;72(1):405–408. doi: 10.1073/pnas.72.1.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELLIOTT W. H. Aminoacetone formation by Staphylococcus aureus. Biochem J. 1960 Mar;74:478–485. doi: 10.1042/bj0740478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fraser J., Newman E. B. Derivation of glycine from threonine in Escherichia coli K-12 mutants. J Bacteriol. 1975 Jun;122(3):810–817. doi: 10.1128/jb.122.3.810-817.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green M. L., Elliott W. H. The enzymic formation of aminoacetone from threonine and its further metabolism. Biochem J. 1964 Sep;92(3):537–549. doi: 10.1042/bj0920537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Isenberg S., Newman E. B. Studies on L-serine deaminase in Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):53–58. doi: 10.1128/jb.118.1.53-58.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lessie T. G., Whiteley H. R. Properties of threonine deaminase from a bacterium able to use threonine as sole source of carbon. J Bacteriol. 1969 Nov;100(2):878–889. doi: 10.1128/jb.100.2.878-889.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
  9. McGilvray D., Morris J. G. Utilization of L-threonine by a species of Arthrobacter. A novel catabolic role for "aminoacetone synthase". Biochem J. 1969 May;112(5):657–671. doi: 10.1042/bj1120657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller B. A., Newman E. B. Control of serine transhydroxymethylase synthesis in Escherichia coli K12. Can J Microbiol. 1974 Jan;20(1):41–47. doi: 10.1139/m74-007. [DOI] [PubMed] [Google Scholar]
  11. Morris J. G. Utilization of L-threnonine by a pseudomonad: a catabolic role for L-threonine aldolase. Biochem J. 1969 Nov;115(3):603–605. doi: 10.1042/bj1150603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NEUBERGER A. Aspects of the metabolism of glycine and of porphyrins. Biochem J. 1961 Jan;78:1–10. doi: 10.1042/bj0780001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PAZ M. A., BLUMENFELD O. O., ROJKIND M., HENSON E., FURFINE C., GALLOP P. M. DETERMINATION OF CARBONYL COMPOUNDS WITH N-METHYL BENZOTHIAZOLONE HYDRAZONE. Arch Biochem Biophys. 1965 Mar;109:548–559. doi: 10.1016/0003-9861(65)90400-5. [DOI] [PubMed] [Google Scholar]
  15. Soffer R. L. Aminoacyl-tRNA transferases. Adv Enzymol Relat Areas Mol Biol. 1974;40(0):91–139. doi: 10.1002/9780470122853.ch4. [DOI] [PubMed] [Google Scholar]
  16. Turner J. M. Microbial metabolism of amino ketones. L-1-aminopropan-2-ol dehydrogenase and L-threonine dehydrogenase in Escherichia coli. Biochem J. 1967 Jul;104(1):112–121. doi: 10.1042/bj1040112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. UMBARGER H. E., BROWN B. Threonine deamination in Escherichia coli. II. Evidence for two L-threonine deaminases. J Bacteriol. 1957 Jan;73(1):105–112. doi: 10.1128/jb.73.1.105-112.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. URATA G., GRANICK S. Biosynthesis of alpha-aminoketones and the metabolism of aminoacetone. J Biol Chem. 1963 Feb;238:811–820. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES