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. 1976 May;126(2):861–868. doi: 10.1128/jb.126.2.861-868.1976

Clo DF13 plasmid deoxyribonucleic acid-directed in vitro synthesis of biologically active cloacin DF13 and clo DF13 immunity protein.

R N Konings, P M Andreoli, E Veltkamp, H J Nijkamp
PMCID: PMC233223  PMID: 770456

Abstract

Clo DF13 plasmid deoxyribonucleic acid (DNA) was used as a template to direct transcription and translation in a DNA-dependent cell-free system prepared from Escherichia coli. Analysis of the invitro products on sodium dodecylsulfate-polyacrylamide gels revealed that Clo DF13 DNA directs the synthesis of at least 10 polypeptides ranging in molecular weight from approximately 7,000 to 70,000. Two of these polypeptides could be identified, with respect to their physiochemical and biological characteristics, as the products of the Clo DF13 genes coding for cloacin DF13 and Clo DF13 immunity protein. These results confirm previous findings, obtained which Clo DF13-harbouring minicells of E. coli, that the structural tenes for the latter proteins residue on the Clo DF13 genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gesteland R. F., Kahn C. Synthesis of bacteriophage lambda proteins in vitro. Nat New Biol. 1972 Nov 1;240(96):3–6. doi: 10.1038/newbio240003a0. [DOI] [PubMed] [Google Scholar]
  2. Hondel C. A., Konings R. N., Schoenmakers J. G. Regulation of gene activity in bacteriophage M13 DNA: Coupled transcription and translation of purified genes and gene-fragments. Virology. 1975 Oct;67(2):487–497. doi: 10.1016/0042-6822(75)90449-3. [DOI] [PubMed] [Google Scholar]
  3. Jakes K. S., Zinder N. D. Highly purified colicin E3 contains immunity protein. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3380–3384. doi: 10.1073/pnas.71.9.3380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Konings R. N., Hulsebos T., Van den Hondel C. A. Identification and characterization of the in vitro synthesized gene products of bacteriophage M13. J Virol. 1975 Mar;15(3):570–584. doi: 10.1128/jvi.15.3.570-584.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Konings R. N., Jansen J., Cuypers T., Schoenmakers J. G. Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein. J Virol. 1973 Dec;12(6):1466–1472. doi: 10.1128/jvi.12.6.1466-1472.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Konings R. N., Schoenmakers J. G. Bacteriophage M13 DNA-directed in vitro synthesis of gene 5 protein. Mol Biol Rep. 1974 Feb;1(5):251–256. doi: 10.1007/BF00417579. [DOI] [PubMed] [Google Scholar]
  7. Konings R. N. Synthesis of phage M13 specific proteins in a DNA-dependent cell-free system. FEBS Lett. 1973 Sep 1;35(1):155–160. doi: 10.1016/0014-5793(73)80600-3. [DOI] [PubMed] [Google Scholar]
  8. Kool A. J., Borstlap A. J., Nijkamp H. J. Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF16: action of the immunity protein in vivo and in vitro. Antimicrob Agents Chemother. 1975 Jul;8(1):76–85. doi: 10.1128/aac.8.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kool A. J., Nijkamp H. J. Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13. J Bacteriol. 1974 Nov;120(2):569–578. doi: 10.1128/jb.120.2.569-578.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kool A. J., Pols C., Nijkamp H. J. Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF13: purification and characterization of the immunity protein. Antimicrob Agents Chemother. 1975 Jul;8(1):67–75. doi: 10.1128/aac.8.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kool A. J., van Zeben M. S., Nijkamp H. J. Identification of messenger ribonucleic acids and proteins synthesized by the bacteriocinogenic factor Clo DF13 in purified minicells of Escherichia coli. J Bacteriol. 1974 Apr;118(1):213–224. doi: 10.1128/jb.118.1.213-224.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Farrell P. Z., Gold L. M. Transcription and translation of prereplicative bacteriophage T4 genes in vitro. J Biol Chem. 1973 Aug 10;248(15):5512–5519. [PubMed] [Google Scholar]
  13. Oudega B., Meekel C. J., De Graaf F. K. Effects of temperature on the activity of cloacin DF13 and cloacin DF13-immunity protein. Biochim Biophys Acta. 1975 Jul 14;399(1):213–216. doi: 10.1016/0304-4165(75)90227-5. [DOI] [PubMed] [Google Scholar]
  14. Stouthamer A. H., Tieze G. A. Bacteriocin production by members of the genus Klebsiella. Antonie Van Leeuwenhoek. 1966;32(2):171–182. doi: 10.1007/BF02097457. [DOI] [PubMed] [Google Scholar]
  15. Veltkamp E., Barendsen W., Nijkamp H. J. Influence of protein and ribonucleic acid synthesis on the replication of the bacteriocinogenic factor Clo DF13 in Escherichia coli cells and minicells. J Bacteriol. 1974 Apr;118(1):165–174. doi: 10.1128/jb.118.1.165-174.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Veltkamp E., Blankevoort R., Nijkamp H. J. Binding of bacteriocin Clo DF13 to Clo DF13 plasmid deoxyribonucleic acid in vivo and in vitro. Antimicrob Agents Chemother. 1975 Sep;8(3):356–365. doi: 10.1128/aac.8.3.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Veltkamp E., Nijkamp H. J. Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication. J Bacteriol. 1974 Dec;120(3):1227–1237. doi: 10.1128/jb.120.3.1227-1237.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Veltkamp E., Nijkamp H. J. The role of DNA polymerase I, II and 3 in the replication of the bacteriocinogenic factor Clo DF 13. Mol Gen Genet. 1973 Sep 27;125(4):329–340. doi: 10.1007/BF00276588. [DOI] [PubMed] [Google Scholar]
  19. de Graaf F. K., Niekus H. G., Klootwijk J. Inactivation of bacterial ribosomes in vivo and in vitro by cloacin DF13. FEBS Lett. 1973 Sep 1;35(1):161–165. doi: 10.1016/0014-5793(73)80601-5. [DOI] [PubMed] [Google Scholar]
  20. de Graaf F. K., Spanjaerdt Speckman E. A., Stouthamer A. H. Mode of action of a bacteriocin produced by Enterobacter cloacae DF13. Antonie Van Leeuwenhoek. 1969;35(3):287–306. doi: 10.1007/BF02219150. [DOI] [PubMed] [Google Scholar]

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