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. 1976 May;126(2):928–936. doi: 10.1128/jb.126.2.928-936.1976

Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.

M Young
PMCID: PMC233231  PMID: 816790

Abstract

Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature, but are prevented from doing so by inhibitors of transcription and translation; this suggests that the product of the defective gene is a protein and that the messenger ribonucleic acid which codes for this protein is short-lived. The other mutant is blocked at stage IV to V and has a long temperature-sensitive period that starts during stage III and precedes the stage at which the mutational defect is phenotypically expressed. The behavior of this mutant in temperature-shift experiments suggests that synthesis of the product of the defective gene commences long before it assumes its physiological function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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