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. 1976 Apr;126(1):282–293. doi: 10.1128/jb.126.1.282-293.1976

Glucose transport in isolated prosthecae of Asticcacaulis biprosthecum.

R J Larson, J L Pate
PMCID: PMC233286  PMID: 4425

Abstract

Active transport of glucose in prosthecae isolated from cells of Asticcacaulis biprosthecum was stimulated by the non-physiological electron donor N, N, N', N'-tetramethyl-p-phenylenediamine dihydrochloride. Glucose uptake was mediated by two transport systems; the apparent Km of the high-affinity system was 1.8 muM and that of the low-affinity system was 34 muM. Free glucose accumulated within prosthecae at a concentration 60 to 200 times above that present externally, depending on the Km of the system being observed. The glucose transport system in prosthecae was stereospecific for D-glucose, and neither methyl alpha-D-glucopyranoside nor 2-deoxyglucose was transported. Uptake of glucose was inhibited by N-ethylmaleimide (NEM) and p-chloromercuribenzoate (PCMB), and the inhibition by PCMB but not by NEM was reversed by dithiothreitol. Glucose uptake was also inhibited by the uncoupling agents 5-chloro-3-t-butyl-2'-nitrosalicylanilide (S-13), 5-chloro-3-(p-chlorophenyl)-4'-chlorosalicylanilide (S-6), and carbonyl-cyanide m-chlorophenylhydrazone (CCCP) and by the respiratory inhibitor KCN. Efflux of glucose from preloaded prosthecae was induced by PCMB and KCN, but not by S-13 or CCCP. Glucose uptake was not affected by arsenate or an inhibitor of membrane-bound adenosine triphosphatases, N, N'-dicyclohexylcarbodiimide. The lack of inhibition by these two compounds, combined with the extremely low levels of adenosine 5'-triphosphate present in prosthecae, indicates that adenosine 5'-triphosphate is not involved in the transport of glucose by prosthecae.

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Selected References

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  1. Barnes E. M., Jr Respiration-coupled glucose transport in membrane vesicles from Azotobacter vinelandii. Arch Biochem Biophys. 1972 Oct;152(2):795–799. doi: 10.1016/0003-9861(72)90275-5. [DOI] [PubMed] [Google Scholar]
  2. Boos W. Bacterial transport. Annu Rev Biochem. 1974;43(0):123–146. doi: 10.1146/annurev.bi.43.070174.001011. [DOI] [PubMed] [Google Scholar]
  3. Guymon L. F., Eagon R. G. Transport of glucose, gluconate, and methyl alpha-D-glucoside by Pseudomonas aeruginosa. J Bacteriol. 1974 Mar;117(3):1261–1269. doi: 10.1128/jb.117.3.1261-1269.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
  5. Holms W. H., Hamilton I. D., Robertson A. G. The rate of turnover of the adenosine triphosphate pool of Escherichia coli growing aerobically in simple defined media. Arch Mikrobiol. 1972;83(2):95–109. doi: 10.1007/BF00425016. [DOI] [PubMed] [Google Scholar]
  6. Hopfer U., Lehninger A. L., Thompson T. E. Protonic conductance across phospholipid bilayer membranes induced by uncoupling agents for oxidative phosphorylation. Proc Natl Acad Sci U S A. 1968 Feb;59(2):484–490. doi: 10.1073/pnas.59.2.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hunter D. R., Segel I. H. Effect of weak acids on amino acid transport by Penicillium chrysogenum: evidence for a proton or charge gradient as the driving force. J Bacteriol. 1973 Mar;113(3):1184–1192. doi: 10.1128/jb.113.3.1184-1192.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jordan T. L., Porter J. S., Pate J. L. Isolation and characterization of prosthecae of Asticcacaulis biprosthecum. Arch Mikrobiol. 1974 Mar 1;96(1):1–16. doi: 10.1007/BF00590158. [DOI] [PubMed] [Google Scholar]
  9. Kaback H. R., Milner L. S. Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1008–1015. doi: 10.1073/pnas.66.3.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaback H. R., Reeves J. P., Short S. A., Lombardi F. J. Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys. 1974 Jan;160(1):215–222. doi: 10.1016/s0003-9861(74)80028-7. [DOI] [PubMed] [Google Scholar]
  11. Kaback H. R. Transport across isolated bacterial cytoplasmic membranes. Biochim Biophys Acta. 1972 Aug 4;265(3):367–416. doi: 10.1016/0304-4157(72)90014-7. [DOI] [PubMed] [Google Scholar]
  12. Kerwar G. K., Gordon A. S., Kaback H. R. Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. J Biol Chem. 1972 Jan 10;247(1):291–297. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Miović M. L., Gibson J. Nucleotide pools and adenylate energy charge in balanced and unbalanced growth of Chromatium. J Bacteriol. 1973 Apr;114(1):86–95. doi: 10.1128/jb.114.1.86-95.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pate J. L., Ordal E. J. The fine structure of two unusual stalked bacteria. J Cell Biol. 1965 Oct;27(1):133–150. doi: 10.1083/jcb.27.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pate J. L., Porter J. S., Jordan T. L. Asticcacaulis biprosthecum sp.nov. Life cycle, morphology and cultural characteristics. Antonie Van Leeuwenhoek. 1973 Nov;39(4):569–583. doi: 10.1007/BF02578901. [DOI] [PubMed] [Google Scholar]
  18. Porter J. S., Pate J. L. Prosthecae of Asticcacaulis biprosthecum: system for the study of membrane transport. J Bacteriol. 1975 Jun;122(3):976–986. doi: 10.1128/jb.122.3.976-986.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reeves J. P., Hong J. S., Kaback H. R. Reconstitution of D-lactate-dependent transport in membrane vesicles from a D-lactate dehydrogenase mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1917–1921. doi: 10.1073/pnas.70.7.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rest R. F., Robertson D. C. Glucose transport in Brucella abortus. J Bacteriol. 1974 Apr;118(1):250–258. doi: 10.1128/jb.118.1.250-258.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Romano A. H., Eberhard S. J., Dingle S. L., McDowell T. D. Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in bacteria. J Bacteriol. 1970 Nov;104(2):808–813. doi: 10.1128/jb.104.2.808-813.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  23. Short S. A., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem. 1974 Jul 10;249(13):4275–4281. [PubMed] [Google Scholar]
  24. Short S. A., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem. 1974 Jul 10;249(13):4275–4281. [PubMed] [Google Scholar]
  25. Sobel M. E., Krulwich T. A. Metabolism of D-fructose by Arthrobacter pyridinolis. J Bacteriol. 1973 Feb;113(2):907–913. doi: 10.1128/jb.113.2.907-913.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson D. F. The stoichiometry and site specificity of the uncoupling of mitochondrial oxidative phosphorylation by salicylanilide derivatives. Biochemistry. 1969 Jun;8(6):2475–2481. doi: 10.1021/bi00834a033. [DOI] [PubMed] [Google Scholar]

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