Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Jan;125(1):68–73. doi: 10.1128/jb.125.1.68-73.1976

Flagellar hook protein from Salmonella SJ25.

H Kagawa, K Owaribe, S Asakura, N Takahashi
PMCID: PMC233336  PMID: 54355

Abstract

From acid-disintegrated flagellar hooks of Salmonella SJ25 an immunochemically pure preparation of hook protein was obtained by column chromatography. The molecular weight of the protein determined by sodium dodecyl sulfate-gel electrophoresis was 43,000, whereas that of SJ25 flagellin was 56,000. The amino-terminal residue of the hook protein was determined to be seryl. The amino acid composition of the protein was determined, the results being very similar to that for an Escheria coli hook protein reported by Silverman and Simon (1972). Within a wavelength range of 200 to 250 nm, our purified preparation of hook protein gave a circular dichroism spectrum with unusually small amplitudes, suggesting that the alpha-helix content of the protein was very low.

Full text

PDF
71

Images in this article

70Image
on p.70
70Image
on p.70

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram D., Mitchen J. R., Koffler H., Vatter A. E. Differentiation within the bacterial flagellum and isolation of the proximal hook. J Bacteriol. 1970 Jan;101(1):250–261. doi: 10.1128/jb.101.1.250-261.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dimmitt K., Simon M. Antigenic nature of bacterial flagellar hook structures. Infect Immun. 1970 Feb;1(2):212–213. doi: 10.1128/iai.1.2.212-213.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dimmitt K., Simon M. Purification and thermal stability of intact Bacillus subtilis flagella. J Bacteriol. 1971 Jan;105(1):369–375. doi: 10.1128/jb.105.1.369-375.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelhoch H. Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry. 1967 Jul;6(7):1948–1954. doi: 10.1021/bi00859a010. [DOI] [PubMed] [Google Scholar]
  5. Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
  6. Hilmen M., Silverman M., Simon M. The regulation of flagellar formation and function. J Supramol Struct. 1974;2(2-4):360–371. doi: 10.1002/jss.400020225. [DOI] [PubMed] [Google Scholar]
  7. Hotani H., Ooi T., Kagawa H., Asakura S., Yamaguchi S. Biochemical evidence for identical primary structure of P-filament and flagellin. Biochim Biophys Acta. 1970 Jul 27;214(1):207–215. doi: 10.1016/0005-2795(70)90085-1. [DOI] [PubMed] [Google Scholar]
  8. ITZHAKI R. F., GILL D. M. A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS. Anal Biochem. 1964 Dec;9:401–410. doi: 10.1016/0003-2697(64)90200-3. [DOI] [PubMed] [Google Scholar]
  9. Kagawa H., Asakura S., Iino T. Serological study of bacterial flagellar hooks. J Bacteriol. 1973 Mar;113(3):1474–1481. doi: 10.1128/jb.113.3.1474-1481.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lawn A. M. Simple immunological labelling method for electron microscopy and its application to the study of filamentous appendages of bacteria. Nature. 1967 Jun 10;214(5093):1151–1152. doi: 10.1038/2141151a0. [DOI] [PubMed] [Google Scholar]
  11. MCDONOUGH M. W. AMINO ACID COMPOSITION OF ANTIGENICALLY DISTINCT SALMONELLA FLAGELLAR PROTEINS. J Mol Biol. 1965 Jun;12:342–355. doi: 10.1016/s0022-2836(65)80258-3. [DOI] [PubMed] [Google Scholar]
  12. Metzger H., Shapiro M. B., Mosimann J. E., Vinton J. E. Assessment of compositional relatedness between proteins. Nature. 1968 Sep 14;219(5159):1166–1168. doi: 10.1038/2191166a0. [DOI] [PubMed] [Google Scholar]
  13. Schmitt R., Raska I., Mayer F. Plain and complex flagella of Pseudomonas rhodos: analysis of fine structure and composition. J Bacteriol. 1974 Feb;117(2):844–857. doi: 10.1128/jb.117.2.844-857.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Silverman M. R., Simon M. I. Flagellar assembly mutants in Escherichia coli. J Bacteriol. 1972 Nov;112(2):986–993. doi: 10.1128/jb.112.2.986-993.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Suzuki H., Iino T. In vitro synthesis of phase-specific flagellin of Salmonella. J Mol Biol. 1973 Nov 25;81(1):57–70. doi: 10.1016/0022-2836(73)90247-7. [DOI] [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES