Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Feb;129(2):1129–1140. doi: 10.1128/jb.129.2.1129-1140.1977

Further mapping of IS2 and IS3 in the lac-purE region of the Escherichia coli K-12 genome: structure of the F-prime ORF203.

R C Deonier, G R Oh, M Hu
PMCID: PMC235054  PMID: 320179

Abstract

The sequence organization of the F-prime ORF203 was determined by heteroduplex analysis. This large, type II F-prime (Scaife, 1967) contains lac, proC, and purE genes derived from the W1485 subline of Escherichia coli K-12. The IS3 and IS2 elements previously found in the lac-proC-purE region derived from the 58-161 subline (Hu et al., 1975) are also present in the same locations in the bacterial deoxyribonucleic acid (DNA) from the W1485 subline. Recombination between the IS2 region of F and an IS2 element located between lac and proC on the bacterial DNA apparently led to the formation of the perental Hfr, OR21. IS2 is thus directly repeated, with one copy of each element appearing at each of the two junctions between F and the bacterial sequences on ORF203. The F plasmid is found together with ORF203 in the plasmid DNA, and this probably forms from ORF203 by recombination between the directly repeated IS2 elements. ORF203 appears to have been excised from the Hfr chromosome by recombination between the IS3 sequence alpha3beta3 located counterclockwise of lac and the directly repeated IS3 sequence alpha4beta4 located clockwise of purE.

Full text

PDF
1132

Images in this article

1133Image
on p.1133
1135Image
on p.1135

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. doi: 10.1146/annurev.mi.23.100169.000441. [DOI] [PubMed] [Google Scholar]
  4. Hu S., Ohtsubo E., Davidson N. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. J Bacteriol. 1975 May;122(2):749–763. doi: 10.1128/jb.122.2.749-763.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hu S., Ptashne K., Cohen S. N., Davidson N. alphabeta sequence of F is IS31. J Bacteriol. 1975 Aug;123(2):687–692. doi: 10.1128/jb.123.2.687-692.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jordan E., Saedler H., Starlinger P. Strong-polar mutations in the transferase gene of the galactose operon in E.coli. Mol Gen Genet. 1967;100(3):296–306. doi: 10.1007/BF00381825. [DOI] [PubMed] [Google Scholar]
  7. Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
  8. Lee H. J., Otsubo E., Deonier R. C., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14. J Mol Biol. 1974 Nov 15;89(4):585–597. doi: 10.1016/0022-2836(74)90037-0. [DOI] [PubMed] [Google Scholar]
  9. Otsubo E., Deonier R. C., Lee H. J., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14. J Mol Biol. 1974 Nov 15;89(4):565–584. doi: 10.1016/0022-2836(74)90036-9. [DOI] [PubMed] [Google Scholar]
  10. Ptashne K., Cohen S. N. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol. 1975 May;122(2):776–781. doi: 10.1128/jb.122.2.776-781.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Saedler H., Heiss B. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12. Mol Gen Genet. 1973 May 9;122(3):267–277. doi: 10.1007/BF00278602. [DOI] [PubMed] [Google Scholar]
  12. Saedler H., Reif H. J., Hu S., Davidson N. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli. Mol Gen Genet. 1974;132(4):265–289. doi: 10.1007/BF00268569. [DOI] [PubMed] [Google Scholar]
  13. Scaife J. Episomes. Annu Rev Microbiol. 1967;21:601–638. doi: 10.1146/annurev.mi.21.100167.003125. [DOI] [PubMed] [Google Scholar]
  14. Shapiro J. A. Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli. J Mol Biol. 1969 Feb 28;40(1):93–105. doi: 10.1016/0022-2836(69)90298-8. [DOI] [PubMed] [Google Scholar]
  15. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  16. Starlinger P., Saedler H. Insertion mutations in microorganisms. Biochimie. 1972;54(2):177–185. doi: 10.1016/s0300-9084(72)80102-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES