Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Apr;130(1):366–374. doi: 10.1128/jb.130.1.366-374.1977

Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

H Niemann, B Kwiatkowski, U Westphal, S Stirm
PMCID: PMC235214  PMID: 853030

Abstract

By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide was elucidated. A glycanase activity, associated with the particles of newly isolated Klebsiella bacteriophage no. 25, was shown to catalyze the hydrolysis of the glycan.

Full text

PDF
371

Images in this article

370Image
on p.370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. Bebault G. M., Choy Y. M., Dutton G. G., Funnell N., Stephen A. M., Yang M. T. Proton magnetic resonance spectroscopy of Klebsiella capsular polysaccharides. J Bacteriol. 1973 Mar;113(3):1345–1347. doi: 10.1128/jb.113.3.1345-1347.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bessler W., Freund-Mölbert E., Knüfermann H., Rduolph C., Thurow H., Stirm S. A bacteriophage-induced depolymerase active on Klebsiella K11 capsular polysaccharide. Virology. 1973 Nov;56(1):134–151. doi: 10.1016/0042-6822(73)90293-6. [DOI] [PubMed] [Google Scholar]
  4. Björndal H., Lindberg B., Lönngren J., Mészáros M., Thompson J. L. Structural studies of the capsular polysaccharide of Klebsiella type 52. Carbohydr Res. 1973 Nov;31(1):93–100. doi: 10.1016/s0008-6215(00)82321-6. [DOI] [PubMed] [Google Scholar]
  5. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Choy Y. M., Fehmel F., Frank N., Stirm S. Escherichia coli capsule bacteriophages. IV. Primary structure of the bacteriophage 29 receptor, the E. coli serotype 29 capsular polysaccharide. J Virol. 1975 Sep;16(3):581–590. doi: 10.1128/jvi.16.3.581-590.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Curvall M., Lindberg B., Lönngren J. Structural studies of the capsular polysaccharide of Klebsiella type 28. Carbohydr Res. 1975 Jun;42(1):95–105. doi: 10.1016/s0008-6215(00)84103-8. [DOI] [PubMed] [Google Scholar]
  8. Curvall M., Lindberg B., Lönngren Structural studies of the capsular polysaccharide of Klebsiella type 81. Carbohydr Res. 1975 Jun;42(1):73–82. doi: 10.1016/s0008-6215(00)84101-4. [DOI] [PubMed] [Google Scholar]
  9. Dutton G. G., Stephen A. M., Churms S. C. Structural investigation of Klebsiella serotype K7 polysaccharide. Carbohydr Res. 1974 Dec;38:225–237. doi: 10.1016/s0008-6215(00)82353-8. [DOI] [PubMed] [Google Scholar]
  10. EDWARDS P. R., FIFE M. A. Capsule types of Klebsiella. J Infect Dis. 1952 Jul-Aug;91(1):92–104. doi: 10.1093/infdis/91.1.92. [DOI] [PubMed] [Google Scholar]
  11. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  12. Heidelberger M., Nimmich W. Immunochemical relationships between bacteria belonging to two separate families: pneumococci and Klebsiella. Immunochemistry. 1976 Jan;13(1):67–80. doi: 10.1016/0019-2791(76)90299-8. [DOI] [PubMed] [Google Scholar]
  13. Hungerer D., Jann K., Jann B., Orskov F., Orskov I. Immunochemistry of K antigens of Escherichia coli. 4. The K antigen of E. coli O 9:K30:H12. Eur J Biochem. 1967 Jul;2(1):115–126. doi: 10.1111/j.1432-1033.1967.tb00115.x. [DOI] [PubMed] [Google Scholar]
  14. Jann K., Jann B., Orskov F., Orskov I., Westphal O. Immunchemische Untersuchungen an K-Antigenen von Escherichia Coli. II. Das K-Antigen von E. coli 08:K42(A):H-. Biochem Z. 1965 Jun 3;342(1):1–22. [PubMed] [Google Scholar]
  15. Le-Ba-Nhan, Jann B., Jann K. Immunochemistry of K antigens of Escherichia coli. The K29 antigen of E. coli 09:K29(A):H-. Eur J Biochem. 1971 Jul 29;21(2):226–234. doi: 10.1111/j.1432-1033.1971.tb01460.x. [DOI] [PubMed] [Google Scholar]
  16. Lindberg B., LönngrenJ, Rudén U. Structural studies of the capsular polysaccharide of Klebsiella type 59. Carbohydr Res. 1975 Jun;42(1):83–93. doi: 10.1016/s0008-6215(00)84102-6. [DOI] [PubMed] [Google Scholar]
  17. Nimmich W. Zur Isolierung und qualitativen Bausteinanalyse der K-Antigene von Klebsiellen. Z Med Mikrobiol Immunol. 1968;154(2):117–131. [PubMed] [Google Scholar]
  18. Rieger D., Freund-Mölbert E., Stirm S. Escherichia coli capsule bacteriophages. III. Fragments of bacteriophage 29. J Virol. 1975 Apr;15(4):964–975. doi: 10.1128/jvi.15.4.964-975.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schlecht S., Westphal O. Wachstum und Lipopolysaccharid (O-Antigen)-Gehalt von Salmonellen bei Züchtung auf Agarnährböden. Zentralbl Bakteriol Orig. 1966 Jun;200(2):241–259. [PubMed] [Google Scholar]
  20. Stirm S., Freund-Mölbert E. Escherichia coli capsule bacteriophages. II. Morphology. J Virol. 1971 Sep;8(3):330–342. doi: 10.1128/jvi.8.3.330-342.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thurow H., Choy Y. M., Frank N., Niemann H., Stirm S. The structure of Klebsiella serotype II capsular polysaccharide. Carbohydr Res. 1975 May;41:241–255. doi: 10.1016/s0008-6215(00)87023-8. [DOI] [PubMed] [Google Scholar]
  22. Thurow H., Niemann H., Rudolph C., Stirm S. Host capsule depolymerase activity of bacteriophage particles active on Klebsiella K20 and K24 strains. Virology. 1974 Mar;58(1):306–309. doi: 10.1016/0042-6822(74)90166-4. [DOI] [PubMed] [Google Scholar]
  23. Thurow H., Niemann H., Stirm S. Bacteriophage-borne enzymes in carbohydrate chemistry. Part I. On the glycanase activity associated with particles of Klebsiella bacteriophage No. 11. Carbohydr Res. 1975 May;41:257–271. doi: 10.1016/s0008-6215(00)87024-x. [DOI] [PubMed] [Google Scholar]
  24. WALLENFELS K., KURZ G. [On the specificity of galactose dehydrogenase from Pseudomonas saccharophila and its use as an analytical aid]. Biochem Z. 1962;335:559–573. [PubMed] [Google Scholar]
  25. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]
  26. ØRSKOV I. O antigens in the Klebsiella group. Acta Pathol Microbiol Scand. 1954;34(2):145–156. doi: 10.1111/j.1699-0463.1954.tb00811.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES