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. 1977 Jun;130(3):1055–1063. doi: 10.1128/jb.130.3.1055-1063.1977

Biosynthesis of wall polymers in Bacillus subtilis.

A W Wyke, J B Ward
PMCID: PMC235327  PMID: 405370

Abstract

Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-diphosphate (UDP)-N-acetylglucosamine followed by addition of glycerol phosphate from cytidine 5'-diphosphate (CDP)-glycerol and finally by addition of ribitol phosphate from CDP-ribitol. UDP-N-acetylglucosamine was not only required for the synthesis of the teichoic acid, but N-acetylglucosamine residues formed an integral part of the linkage unit attaching polyribitol phosphate to the cell wall. Synthesis of the teichoic acid was exquisitely sensitive to the antibiotic tunicamycin, and this was shown to be due to the inhibition of incorporation of N-acetylglucosamine units from UDP-N-acetylglucosamine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. Anderson J. S., Matsuhashi M., Haskin M. A., Strominger J. L. Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967 Jul 10;242(13):3180–3190. [PubMed] [Google Scholar]
  3. Anderson R. G., Hussey H., Baddiley J. The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane. Biochem J. 1972 Mar;127(1):11–25. doi: 10.1042/bj1270011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Archibald A. R., Baddiley J., Button D. The glycerol teichoic acid of walls of Staphylococcus lactis I3. Biochem J. 1968 Dec;110(3):543–557. doi: 10.1042/bj1100543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bracha R., Glaser L. An intermediate in telchoic acid biosynthesis. Biochem Biophys Res Commun. 1976 Oct 4;72(3):1091–1098. doi: 10.1016/s0006-291x(76)80244-6. [DOI] [PubMed] [Google Scholar]
  6. Bracha R., Glaser L. In vitro system for the synthesis of teichoic acid linked to peptidoglycan. J Bacteriol. 1976 Mar;125(3):872–879. doi: 10.1128/jb.125.3.872-879.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Button D., Archibald A. R., Baddiley J. The linkage between teichoic acid and glycosaminopeptide in the walls of a strain of Staphylococcus lactis. Biochem J. 1966 May;99(2):11C–14C. doi: 10.1042/bj0990011c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coley J., Archibald A. R., Baddiley J. A linkage unit joining peptidoglycan to teichoic acid in Staphylococcus aureus H. FEBS Lett. 1976 Jan 15;61(2):240–242. doi: 10.1016/0014-5793(76)81047-2. [DOI] [PubMed] [Google Scholar]
  9. Durda P. J., Cynkin M. A. A procedure for the separation of glucosamine from glucosaminitol. Anal Biochem. 1974 Jun;59(2):407–409. doi: 10.1016/0003-2697(74)90292-9. [DOI] [PubMed] [Google Scholar]
  10. Fiedler F., Glaser L. The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier. J Biol Chem. 1974 May 10;249(9):2684–2689. [PubMed] [Google Scholar]
  11. GLASER L. THE SYNTHESIS OF TEICHOIC ACIDS. II. POLYRIBITOL PHOSPHATE. J Biol Chem. 1964 Oct;239:3178–3186. [PubMed] [Google Scholar]
  12. Garrett A. J. The effect of magnesium ion deprivation on the synthesis of mucopeptide and its precursors in Bacillus subtilis. Biochem J. 1969 Nov;115(3):419–430. doi: 10.1042/bj1150419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hammes W. P., Neuhaus F. C. On the specificity of phospho-N-acetylmuramyl-pentapeptide translocase. The peptide subunit of uridine diphosphate-N-actylmuramyl-pentapeptide. J Biol Chem. 1974 May 25;249(10):3140–3150. [PubMed] [Google Scholar]
  14. Hancock I. C., Wiseman G., Baddiley J. Biosynthesis of the unit that links teichoic acid to the bacterial wall: inhibition by tunicamycin. FEBS Lett. 1976 Oct 15;69(1):75–80. doi: 10.1016/0014-5793(76)80657-6. [DOI] [PubMed] [Google Scholar]
  15. Hancock I., Baddiley J. In vitro synthesis of the unit that links teichoic acid to peptidoglycan. J Bacteriol. 1976 Mar;125(3):880–886. doi: 10.1128/jb.125.3.880-886.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lehle L., Tanner W. The specific site of tunicamycin inhibition in the formation of dolichol-bound N-acetylglucosamine derivatives. FEBS Lett. 1976 Nov 15;72(1):167–170. doi: 10.1016/0014-5793(76)80922-2. [DOI] [PubMed] [Google Scholar]
  18. Mauck J., Glaser L. On the mode of in vivo assembly of the cell wall of Bacillus subtilis. J Biol Chem. 1972 Feb 25;247(4):1180–1187. [PubMed] [Google Scholar]
  19. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  20. Mirelman D., Bracha R., Sharon N. Penicillin-induced secretion of soluble, uncross-linked peptidoglycan by Micrococcus luteus cells. Biochemistry. 1974 Nov 19;13(24):5045–5053. doi: 10.1021/bi00721a028. [DOI] [PubMed] [Google Scholar]
  21. SHAW D. R. Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol. Biochem J. 1962 Feb;82:297–312. doi: 10.1042/bj0820297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tkacz J. S., Lampen O. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes. Biochem Biophys Res Commun. 1975 Jul 8;65(1):248–257. doi: 10.1016/s0006-291x(75)80086-6. [DOI] [PubMed] [Google Scholar]
  23. Tomasz A., McDonnell M., Westphal M., Zanati E. Coordinated incorporation of nascent peptidoglycan and teichoic acid into pneumococcal cell walls and conservation of peptidoglycan during growth. J Biol Chem. 1975 Jan 10;250(1):337–341. [PubMed] [Google Scholar]
  24. Toon P., Brown P. E., Baddiley J. The lipid-teichoic acid complex in the cytoplasmic membrane of Streptococcus faecalis N.C.I.B. 8191. Biochem J. 1972 Apr;127(2):399–409. doi: 10.1042/bj1270399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tynecka Z., Ward J. B. Peptidoglycan synthesis in Bacillus licheniformis. The inhibition of cross-linking by benzylpenicillin and cephaloridine in vivo accompanied by the formation of soluble peptidoglycan. Biochem J. 1975 Jan;146(1):253–267. doi: 10.1042/bj1460253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ward J. B., Perkins H. R. The direction of glycan synthesis in a bacterial peptidoglycan. Biochem J. 1973 Dec;135(4):721–728. doi: 10.1042/bj1350721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ward J. B. The synthesis of peptidoglycan in an autolysin-deficient mutant of Bacillus licheniformis N.C.T.C. 6346 and the effect of beta-lactam antibiotics, bacitracin and vancomycin. Biochem J. 1974 Jul;141(1):227–241. doi: 10.1042/bj1410227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wyke A. W., Ward J. B. The biosynthesis of muramic acid phosphate in Bacillus licheniformis. FEBS Lett. 1977 Feb 1;73(2):159–163. doi: 10.1016/0014-5793(77)80971-x. [DOI] [PubMed] [Google Scholar]
  29. Wyke A. W., Ward J. B. The synthesis of covalently-linked teichoic acid and peptidoglycan by cell-free preparations of Bacillus licheniformis. Biochem Biophys Res Commun. 1975 Aug 4;65(3):877–885. doi: 10.1016/s0006-291x(75)80467-0. [DOI] [PubMed] [Google Scholar]

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