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. 1977 Jul;131(1):105–110. doi: 10.1128/jb.131.1.105-110.1977

Characterization of methylated neutral amino acids from Escherichia coli ribosomes.

F N Chang, C Budzilowicz
PMCID: PMC235397  PMID: 326752

Abstract

The methylated neutral amino acids from both 30S and 50S ribosomal subunits of an Escherichia coli K strain were characterized. The 50S ribosomal subunit contains three methylated neutral amino acids: N-monomethylalanine, N-monomethylmethionine, and an as yet unidentified methylated amino acid found in protein L11. Both N-monomethylalanine and N-monomethylmethionine were found in protein L33. The amount of N-monomethylmethionine in this protein, however, is variable but not more than 0.25 molecules per protein. Thus protein L33 from this E. coli K strain has heterogeneity in its N-terminal amino acid and can start with either N-monomethylalanine or N-monomethylmethionine. The N-monomethylmethionine residue was not derived from the reduction of N-formylmethionine in the protein. The 30S ribosomal subunit contains only one methylated neutral amino acid: N-monomethylalanine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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