Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Jul;131(1):179–187. doi: 10.1128/jb.131.1.179-187.1977

Glucose catabolism in two derivatives of a Rhizobium japonicum strain differing in nitrogen-fixing efficiency.

K Mulongoy, G H Elkan
PMCID: PMC235407  PMID: 559670

Abstract

Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pathways. The hexose cycle may play a minor role in the dissimilation of glucose. Failure to detect the nicotinamide adenine dinucleotide phosphate-dependent decarboxylating 6-phosphogluconate dehydrogenase (EC 1.1.1.44) evidences absence of the pentose phosphate pathway. Transketolase and transaldolase reactions, however, enable R. japonicum to produce the precursors for purine and pyrimidine biosynthesis from fructose-6-phosphate and glyceraldehyde-3-phosphate. A constitutive nicotinamide adenine dinucleotide-linked 6-phosphogluconate dehydrogenase has been detected. The enzyme is stimulated by either mannitol or fuctose and might initiate a new catabolic pathway. R. japonicum isolate I-110, characterized by shorter generation times on glucose and greater nitrogen-fixing efficiency, oxidizes glucose more extensively than type L1-110 and utilizes preferentially the Embden-Meyerhof-Parnas pathway, whereas the Entner-Doudoroff pathway apparently predominates in type L1-110.

Full text

PDF
179

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cole M. A., Elkan G. H. Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum. Antimicrob Agents Chemother. 1973 Sep;4(3):248–253. doi: 10.1128/aac.4.3.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eisenberg R. C., Dobrogosz W. J. Gluconate metabolism in Escherichia coli. J Bacteriol. 1967 Mar;93(3):941–949. doi: 10.1128/jb.93.3.941-949.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. JORDAN D. C. The bacteroids of the genus Rhizobium. Bacteriol Rev. 1962 Jun;26:119–141. [PMC free article] [PubMed] [Google Scholar]
  4. KATZNELSON H., ZAGALLO A. C. Metabolism of rhizobia in relation to effectiveness. Can J Microbiol. 1957 Oct;3(6):879–884. doi: 10.1139/m57-097. [DOI] [PubMed] [Google Scholar]
  5. Keele B. B., Jr, Hamilton P. B., Elkan G. H. Gluconate catabolism in Rhizobium japonicum. J Bacteriol. 1970 Mar;101(3):698–704. doi: 10.1128/jb.101.3.698-704.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keele B. B., Jr, Hamilton P. B., Elkan G. H. Glucose catabolism in Rhizobium japonicum. J Bacteriol. 1969 Mar;97(3):1184–1191. doi: 10.1128/jb.97.3.1184-1191.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuykendall L. D., Elkan G. H. Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. Appl Environ Microbiol. 1976 Oct;32(4):511–519. doi: 10.1128/aem.32.4.511-519.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laughon B. E., Krieg N. R. Sugar catabolism in Aquaspirillum gracile. J Bacteriol. 1974 Sep;119(3):691–697. doi: 10.1128/jb.119.3.691-697.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Martínez-De Drets G., Arias A. Enzymatic basis for differentiation of Rhizobium into fast- and slow-growing groups. J Bacteriol. 1972 Jan;109(1):467–470. doi: 10.1128/jb.109.1.467-470.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ragland T. E., Kawasaki T., Lowenstein J. M. Comparative aspects of some bacterial dehydrogenases and transhydrogenases. J Bacteriol. 1966 Jan;91(1):236–244. doi: 10.1128/jb.91.1.236-244.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Raj H. D. Radiorespirometric studies of Leucothrix mucor. J Bacteriol. 1967 Sep;94(3):615–623. doi: 10.1128/jb.94.3.615-623.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STILL G. G., WANG C. H. GLUCOSE CATABOLISM IN AZOTOBACTER VINELANDII. Arch Biochem Biophys. 1964 Apr;105:126–132. doi: 10.1016/0003-9861(64)90243-7. [DOI] [PubMed] [Google Scholar]
  14. Vander Wyk J. C., Lessie T. G. Purification and characterization of the Pseudomonas multivorans glucose-6-phosphate dehydrogenase active with nicotinamide adenine dinucleotide. J Bacteriol. 1974 Dec;120(3):1033–1042. doi: 10.1128/jb.120.3.1033-1042.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES