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. 1977 Jul;131(1):224–228. doi: 10.1128/jb.131.1.224-228.1977

Detection and characterization of plasmids in Pseudomonas glycinea.

M S Curiale, D Mills
PMCID: PMC235413  PMID: 873883

Abstract

Pathogenic strains of Pseudomonas glycinea were shown to possess plasmid deoxyribonucleic acid by dye-buoyant density gradient centrifugation. The size and number of plasmids of four different isolates were determined by neutral sucrose gradient centrifugation. Two isolates were found to harbor a single plasmid; however, they differed in size, having molecular weights of 43 X 10(6) and 54 X 10(6). Two other isolates each contained two different plasmids. Plasmids with molecular weights of 43 X 10(6) and 73 X 10(6) were observed in one isolate, and the other carried plasmids with molecular weights of 25 X 10(6) and 87 X 10(6). An auxotrophic mutant derived from the latter strain was found to contain plasmids of identical size. The plasmids were found to be under stringent control of replication, having plasmid copies of 1.0 to 2.7 per chromosome equivalent. By the dye-cesium chloride technique, the mutant showed twice as much covalently closed circular deoxyribonucleic acid as did the parental strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth P. T., Grinter N. J. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. J Bacteriol. 1974 Nov;120(2):618–630. doi: 10.1128/jb.120.2.618-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carrier W. L., Setlow R. B. Paper strip method for assaying gradient fractions containing radioactive macromolecules. Anal Biochem. 1971 Oct;43(2):427–432. doi: 10.1016/0003-2697(71)90272-7. [DOI] [PubMed] [Google Scholar]
  3. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cress D. E., Kline B. C. Isolation and characterization of Escherichia coli chromosomal mutants affecting plasmid copy number. J Bacteriol. 1976 Feb;125(2):635–642. doi: 10.1128/jb.125.2.635-642.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Currier T. C., Nester E. W. Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium. J Bacteriol. 1976 Apr;126(1):157–165. doi: 10.1128/jb.126.1.157-165.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Merlo D. J., Nester E. W. Plasmids in avirulent strains of Agrobacterium. J Bacteriol. 1977 Jan;129(1):76–80. doi: 10.1128/jb.129.1.76-80.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Park I. W., De Ley J. Ancestral remnants in deoxyribonucleic acid from Pseudomonas and Xanthomonas. Antonie Van Leeuwenhoek. 1967;33(1):1–16. doi: 10.1007/BF02045528. [DOI] [PubMed] [Google Scholar]
  9. Pemberton J. M., Clark A. J. Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973 Apr;114(1):424–433. doi: 10.1128/jb.114.1.424-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Uhlin B. E., Nordström K. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12. J Bacteriol. 1975 Nov;124(2):641–649. doi: 10.1128/jb.124.2.641-649.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vidaver A. K., Mathys M. L., Thomas M. E., Schuster M. L. Bacteriocins of the phytopathogens Pseudomonas syringae, P. glycinea, and P. phaseolicola. Can J Microbiol. 1972 Jun;18(6):705–713. doi: 10.1139/m72-113. [DOI] [PubMed] [Google Scholar]
  12. Watson B., Currier T. C., Gordon M. P., Chilton M. D., Nester E. W. Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1975 Jul;123(1):255–264. doi: 10.1128/jb.123.1.255-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zaenen I., Van Larebeke N., Van Montagu M., Schell J. Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol. 1974 Jun 15;86(1):109–127. doi: 10.1016/s0022-2836(74)80011-2. [DOI] [PubMed] [Google Scholar]

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