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. 1977 Jul;131(1):374–377. doi: 10.1128/jb.131.1.374-377.1977

Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.

I Mahler, H O Halvorson
PMCID: PMC235434  PMID: 406259

Abstract

A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine prototrophy, but fails to survive in strains of B. subtilis 168.

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Selected References

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  1. Bott K. F., Wilson G. A. Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967 Sep;94(3):562–570. doi: 10.1128/jb.94.3.562-570.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Dubnau D., Cirigliano C. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA. J Mol Biol. 1972 Feb 28;64(1):9–29. doi: 10.1016/0022-2836(72)90318-x. [DOI] [PubMed] [Google Scholar]
  4. Ehrlich S. D., Bursztyn-Pettegrew H., Stroynowski I., Lederberg J. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145–4149. doi: 10.1073/pnas.73.11.4145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris-Warrick R. M., Elkana Y., Ehrlich S. D., Lederberg J. Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207–2211. doi: 10.1073/pnas.72.6.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455–3459. doi: 10.1073/pnas.71.9.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morrison D. A., Guild W. R. Activity of deoxyribonucleic acid fragments of defined size in Bacillus subtilis transformation. J Bacteriol. 1972 Oct;112(1):220–223. doi: 10.1128/jb.112.1.220-223.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  10. Shapiro J. A., Dean D. H., Halvorson H. O. Low-frequency specialized transduction with Bacillus subtilis bacteriophage phi 105. Virology. 1974 Dec;62(2):393–403. doi: 10.1016/0042-6822(74)90401-2. [DOI] [PubMed] [Google Scholar]
  11. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  12. Trautner T. A., Pawlek B., Bron S., Anagnostopoulos C. Restriction and modification in B. subtilis. Biological aspects. Mol Gen Genet. 1974;131(3):181–191. doi: 10.1007/BF00267958. [DOI] [PubMed] [Google Scholar]
  13. Wensink P. C., Finnegan D. J., Donelson J. E., Hogness D. S. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell. 1974 Dec;3(4):315–325. doi: 10.1016/0092-8674(74)90045-2. [DOI] [PubMed] [Google Scholar]

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