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. 1975 Sep;123(3):1254–1264. doi: 10.1128/jb.123.3.1254-1264.1975

Deletions fusing the hisG and hisD genes in Salmonella typhimurium.

I Ino, P E Hartman, Z Hartman, J Yourno
PMCID: PMC235849  PMID: 1099075

Abstract

Frameshift mutation hisD497 occurs in the operator-proximal portion of the Salmonella typhimurium gene coding for the dimeric protein, L-histidinol dehydrogenase (HDH). Rare revertants of hisD497 are deletions fusing the hisD gene to the adjacent preceding structural gene, hisG (adenosine 5'-triphosphate-PR transferase). HDH purified from one revertant, hisGD4908, contains subunits of approximately normal molecular weight but with no clearly demonstrable unique amino-terminal sequence. We propose that a combined inactive G-D polypeptide is synthesized and then cleaved at a number of closely juxtaposed sites by endoproteolytic activity. At least some of the resulting fragments then participate in formation of active HDH dimers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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