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. 1975 Oct;124(1):26–38. doi: 10.1128/jb.124.1.26-38.1975

Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.

J Lengeler
PMCID: PMC235860  PMID: 1100602

Abstract

Mutants of Escherichia coli K-12 unable to grow on any of the three naturally occurring hexitols D-manitol, D-glucitol, and galactitol and, among these specifically, mutants with altered transport and phosphorylating activity have been isolated. Different isolation procedures have been utilized, including suicide by D-[3H]mannitol, chemotaxis, and resistance to the toxic hexitol analogue 2-deoxy-arabino-hexitol. Mutations thus obtained have been mapped in four distinct operons. (i) Mutations affecting an enzyme II-complexmt1 activity of the phosphoenolpyruvate-dependent phosphotransferase system all map in gene mtlA. This gene has previously been shown (Solomon and Lin, 1972) to be part of an operon, mtl, located at 71 min on the E. coli linkage map containing, in addition to mtlA, the cis-dominant regulatory gene mtlC and mtlD, the structural gene for the enzyme D-mannitol-1-phosphate dehydrogenase. The gene order in this operon, induced by D-mannitol, is mtlC A D. (ii) Mutations in gene gutA affecting a second enzyme II-complexgut of the phosphotransferase system map at 51 min, clustered in operon gutC A D together with the cis-dominant regulatory gene gutC and the structural gene gutD for the enzyme D-glucitol-6-phosphate dehydrogenase. The gut operon, previously called sbl or srl, is induced by D-glucitol. (iii) Mutations affecting the transport and catabolism of galactitol are clustered in a third operon, gatC A D, located at 40.5 min. This operon again contains a cis-dominant regulatory gene, gatC, the structural gene gatD for galactitol-1-phosphate dehydrogenase, and gene gatA coding for a thrid hexitol-specific enzyme II-complexgat. Other genes coding for two additional enzymes involved in galactitol catabolism apparently are not linked to gatC A D. (iv) A fourth class of mutants pleiotropically negative for hexitol growth and transport maps in the pts operon. Triple-negative mutants (mtlA gutA gatA) do not have further transport or phosphorylating activity for any of the three hexitols.

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Selected References

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  1. ARBER W. Transduction of chromosomal genes and episomes in Escherichia coli. Virology. 1960 May;11:273–288. doi: 10.1016/0042-6822(60)90066-0. [DOI] [PubMed] [Google Scholar]
  2. Adler J., Epstein W. Phosphotransferase-system enzymes as chemoreceptors for certain sugars in Escherichia coli chemotaxis. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2895–2899. doi: 10.1073/pnas.71.7.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adler J., Hazelbauer G. L., Dahl M. M. Chemotaxis toward sugars in Escherichia coli. J Bacteriol. 1973 Sep;115(3):824–847. doi: 10.1128/jb.115.3.824-847.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berkowitz D. D-Mannitol utilization in Salmonella typhimurium. J Bacteriol. 1971 Jan;105(1):232–240. doi: 10.1128/jb.105.1.232-240.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLARK A. J. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics. 1963 Jan;48:105–120. doi: 10.1093/genetics/48.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Charnetzky W. T., Mortlock R. P. D-Arabitol catabolic pathway in Klebsiella aerogenes. J Bacteriol. 1974 Jul;119(1):170–175. doi: 10.1128/jb.119.1.170-175.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dahl R., Morse H. G., Morse M. L. Carbohydrate transport and cyclic 3',5' adenosine monophosphate (cAMP) levels in a temperature sensitive phosphotransferase mutant of Escherichia coli. Mol Gen Genet. 1974 Mar 6;129(1):1–10. doi: 10.1007/BF00269261. [DOI] [PubMed] [Google Scholar]
  8. Doelle H. W. Dimeric and tetrameric phosphofructokinase and the Pasteur effect in Escherichia coli K-12. FEBS Lett. 1974 Dec 15;49(2):220–222. doi: 10.1016/0014-5793(74)80516-8. [DOI] [PubMed] [Google Scholar]
  9. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  10. KORN E. D. Clearing factor, a heparin-activated lipoprotein lipase. I. Isolation and characterization of the enzyme from normal rat heart. J Biol Chem. 1955 Jul;215(1):1–14. [PubMed] [Google Scholar]
  11. Kelker N. E., Anderson R. L. Sorbitol metabolism in Aerobacter aerogenes. J Bacteriol. 1971 Jan;105(1):160–164. doi: 10.1128/jb.105.1.160-164.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klungsoyr L. Mannitol kinase in cell-free extracts of Escherichia coli. Biochim Biophys Acta. 1966 Aug 10;122(2):361–364. [PubMed] [Google Scholar]
  13. LUBIN M. Selection of auxotrophic bacterial mutants by tritium-labeled thymidine. Science. 1959 Mar 27;129(3352):838–839. doi: 10.1126/science.129.3352.838. [DOI] [PubMed] [Google Scholar]
  14. Lengeler J., Lin E. C. Reversal of the mannitol-sorbitol diauxie in Escherichia coli. J Bacteriol. 1972 Nov;112(2):840–848. doi: 10.1128/jb.112.2.840-848.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lengeler J. Nature and properties of hexitol transport systems in Escherichia coli. J Bacteriol. 1975 Oct;124(1):39–47. doi: 10.1128/jb.124.1.39-47.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MUNKRES K. D., RICHARDS F. M. THE PURIFICATION AND PROPERTIES OF NEUROSPORA MALATE DEHYDROGENASE. Arch Biochem Biophys. 1965 Mar;109:466–479. doi: 10.1016/0003-9861(65)90391-7. [DOI] [PubMed] [Google Scholar]
  18. Ordal G. W., Adler J. Isolation and complementation of mutants in galactose taxis and transport. J Bacteriol. 1974 Feb;117(2):509–516. doi: 10.1128/jb.117.2.509-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ruch F. E., Lengeler J., Lin E. C. Regulation of glycerol catabolism in Klebsiella aerogenes. J Bacteriol. 1974 Jul;119(1):50–56. doi: 10.1128/jb.119.1.50-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ruffler D., Buckel P., Piepersberg W., Böck A. Alanyl-tRNA synthetase of Escherichia coli: genetic analysis of the structural gene and of suppressor mutations. Mol Gen Genet. 1974;134(4):313–323. doi: 10.1007/BF00337466. [DOI] [PubMed] [Google Scholar]
  21. Schreyer R., Böck A. Phenotypic suppression of a fructose-1,6-diphosphate aldolase mutation in Escherichia coli. J Bacteriol. 1973 Jul;115(1):268–276. doi: 10.1128/jb.115.1.268-276.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Solomon E., Lin E. C. Mutations affecting the dissimilation of mannitol by Escherichia coli K-12. J Bacteriol. 1972 Aug;111(2):566–574. doi: 10.1128/jb.111.2.566-574.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WOLFF J. B., KAPLAN N. O. D-Mannitol 1-phosphate dehydrogenase from Escherichia coli. J Biol Chem. 1956 Feb;218(2):849–869. [PubMed] [Google Scholar]
  26. WOLFF J. B., KAPLAN N. O. Hexitol metabolism in Escherichia coli. J Bacteriol. 1956 May;71(5):557–564. doi: 10.1128/jb.71.5.557-564.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]

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