Abstract
Klebsiella aerogenes dissimilates glycerol aerobically via an inducible pathway initiated by an adenosine triphosphate-linked kinase that converts the substrate to sn-glycerol 3-phosphate. Phosphorylated glycerol is then dehydrogenated to dihydroxyacetone phosphate by an enzyme characteristic of a flavoprotein. Anaerobically, the organism dissimilates glycerol via an inducible pathway initiated by a nicotinamide adenine dinucleotide-linked dehydrogenase that converts the substrate to dihydroxyacetone. The keto product is then phosphorylated by another adenosine triphosphate-linked kinase. Two kinds of constitutive mutants have been isolated: one affecting the aerobic and the other the anaerobic pathway.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
- Freedberg W. B., Lin E. C. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J Bacteriol. 1973 Sep;115(3):816–823. doi: 10.1128/jb.115.3.816-823.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOCH J. P., HAYASHI S., LIN E. C. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3106–3108. [PubMed] [Google Scholar]
- LIN E. C., LERNER S. A., JORGENSEN S. E. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochim Biophys Acta. 1962 Jul 2;60:422–424. doi: 10.1016/0006-3002(62)90423-7. [DOI] [PubMed] [Google Scholar]
- LIN E. C., LEVIN A. P., MAGASANIK B. The effect of aerobic metabolism on the inducible glycerol dehydrogenase of Aerobacter aerogenes. J Biol Chem. 1960 Jun;235:1824–1829. [PubMed] [Google Scholar]
- MAGASANIK B., BROOKE M. S., KARIBIAN D. Metabolic pathways of glycerol dissimilation; a comparative study of two strains of Aerobacter aerogenes. J Bacteriol. 1953 Nov;66(5):611–619. doi: 10.1128/jb.66.5.611-619.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruch F. E., Lengeler J., Lin E. C. Regulation of glycerol catabolism in Klebsiella aerogenes. J Bacteriol. 1974 Jul;119(1):50–56. doi: 10.1128/jb.119.1.50-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]