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. 1975 Oct;124(1):373–379. doi: 10.1128/jb.124.1.373-379.1975

Effects of temperature and nutritional changes on the fatty acids of agmenellum quadruplicatum.

G J Olson, L O Ingram
PMCID: PMC235905  PMID: 809416

Abstract

The fatty acid composition of the blue-green bacterium Agmenellum quadruplicatum was examined under a wide variety of growth conditions. The fatty acid composition was found to undergo significant changes with variations in temperature, media composition, and growth phase (log versus stationary). With increasing growth temperature (20 to 43 C) log-phase cells exhibited an increase in saturated fatty acids (38.4% at 20 C to 63.6% at 43 C). Striking changes were seen with some of the individual fatty acids such as 18.3, which made up 16.0% of the total fatty acid at 20 C but was not neasurable at 43 C. Fatty acid 12:0 was not measurable at 20 C but made up 16.3% of the total fatty acids at 43 C. Cell lipids were separated into neutral lipid, glycolipid, and very polar liquid fractions. The neutral lipid fraction was composed almost entirely of 12 carbon fatty acids (12:0, 12:1). Glycolipid and very polar lipids were more similar in their fatty acid composition when compared to the total cellular fatty acids, although they did lack 12 carbon fatty acids. The total of 12 carbon fatty acids in the cell can be used as an indicator of the amount of neutral lipid present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown C. M., Rose A. H. Fatty-acid composition of Candida utilis as affected by growth temperature and dissolved-oxygen tension. J Bacteriol. 1969 Aug;99(2):371–378. doi: 10.1128/jb.99.2.371-378.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  3. Freeman C. P., West D. Complete separation of lipid classes on a single thin-layer plate. J Lipid Res. 1966 Mar;7(2):324–327. [PubMed] [Google Scholar]
  4. Fulco A. J. Metabolic alterations of fatty acids. Annu Rev Biochem. 1974;43(0):215–241. doi: 10.1146/annurev.bi.43.070174.001243. [DOI] [PubMed] [Google Scholar]
  5. Goldfine H. Comparative aspects of bacterial lipids. Adv Microb Physiol. 1972;8:1–58. doi: 10.1016/s0065-2911(08)60187-3. [DOI] [PubMed] [Google Scholar]
  6. Harris P., James A. T. The effect of low temperatures on fatty acid biosynthesis in plants. Biochem J. 1969 Apr;112(3):325–330. doi: 10.1042/bj1120325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holton R. W., Blecker H. H., Stevens T. S. Fatty acids in blue-green algae: possible relation to phylogenetic position. Science. 1968 May 3;160(3827):545–547. doi: 10.1126/science.160.3827.545. [DOI] [PubMed] [Google Scholar]
  8. Ingram L. O., Van Baalen C. Characteristics of a stable, filamentous mutant of a coccoid blue-green alga. J Bacteriol. 1970 Jun;102(3):784–789. doi: 10.1128/jb.102.3.784-789.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  10. KATES M., BAXTER R. M. Lipid composition of mesophilic and psychrophilic yeasts (Candida species) as influenced by environmental temperature. Can J Biochem Physiol. 1962 Sep;40:1213–1227. [PubMed] [Google Scholar]
  11. Kenyon C. N. Fatty acid composition of unicellular strains of blue-green algae. J Bacteriol. 1972 Feb;109(2):827–834. doi: 10.1128/jb.109.2.827-834.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kenyon C. N., Rippka R., Stanier R. Y. Fatty acid composition and physiological properties of some filamentous blue-green algae. Arch Mikrobiol. 1972;83(3):216–236. doi: 10.1007/BF00645123. [DOI] [PubMed] [Google Scholar]
  13. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Naccarato W. F., Gilbertson J. R., Gelman R. A. Effects of different culture media and oxygen upon lipids of Escherichia coli K-12. Lipids. 1974 May;9(5):322–327. doi: 10.1007/BF02533108. [DOI] [PubMed] [Google Scholar]
  15. Shaw N. Lipid composition as a guide to the classification of bacteria. Adv Appl Microbiol. 1974;17(0):63–108. doi: 10.1016/s0065-2164(08)70555-0. [DOI] [PubMed] [Google Scholar]
  16. Silbert D. F., Ladenson R. C., Honegger J. L. The unsaturated fatty acid requirement in Escherichia coli. Temperature dependence and total replacement by branched-chain fatty acids. Biochim Biophys Acta. 1973 Jul 6;311(3):349–361. doi: 10.1016/0005-2736(73)90315-5. [DOI] [PubMed] [Google Scholar]
  17. VORBECK M. L., MARINETTI G. V. SEPARATION OF GLYCOSYL DIGLYCERIDES FROM PHOSPHATIDES USING SILICIC ACID COLUMN CHROMATOGRAPHY. J Lipid Res. 1965 Jan;6:3–6. [PubMed] [Google Scholar]
  18. Yribarren M., Vilkas E. Galactosyl-diglyceride from Actinomyces viscosus. Chem Phys Lipids. 1974 May;12(3):172–175. doi: 10.1016/0009-3084(74)90072-3. [DOI] [PubMed] [Google Scholar]

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