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. 1975 Nov;124(2):597–601. doi: 10.1128/jb.124.2.597-601.1975

Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.

S Iordănescu
PMCID: PMC235944  PMID: 1184573

Abstract

From two different, compatible staphylococcal plasmids that determine streptomycin and chloramphenicol resistance, respectively, a recombinant plasmid was obtained. This plasmid can be transduced with a rather high frequency (10(-4)/plaque-forming unit) to plasmid-negative strains, the linkage of the two markers being 100%. The maintenance of the recombinant plasmid in the host cell seems to be controlled by the chloramphenicol resistance plasmid. The recombinant plasmid proved to be incompatible with both parental plasmids, which are unrelated. The relationship between the chloramphenicol resistance plasmid and the recombinant plasmid was the same as the between genetically marked derivatives of the recombinant plasmid, whereas the relationship of the streptomycin resistance plasmid to the recombinant plasmid was of a different, asymmetrical type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S. Recombination between unrelated bacterial plasmids. Ann Microbiol (Paris) 1974 Apr;125A(3):251–259. [PubMed] [Google Scholar]
  2. Chakrabarty A. M. Genetic fusion of incompatible plasmids in Pseudomonas. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1641–1644. doi: 10.1073/pnas.70.6.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chou G. I., Katz D., Gunsalus I. C. Fusion and compatibility of camphor and octane plasmids in Pseudomonas. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2675–2678. doi: 10.1073/pnas.71.7.2675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coetzee J. N. Properties of proteus and providence strains harbouring recombinant plasmids between P-lac Rldrd19 or R447b. J Gen Microbiol. 1974 Jan;80(1):119–130. doi: 10.1099/00221287-80-1-119. [DOI] [PubMed] [Google Scholar]
  5. Cooper P. Interaction of a colicinogenic factor with a resistance factor and with the fertility factor F in Escherichia coli K-12. Genet Res. 1971 Apr;17(2):151–159. doi: 10.1017/s0016672300012143. [DOI] [PubMed] [Google Scholar]
  6. Datta N., Hedges R. W. An I pilus-determining R factor with anomalous compatibility properties, mobilizing a gentamicin-resistance plasmid. J Gen Microbiol. 1973 Jul;77(1):11–17. doi: 10.1099/00221287-77-1-11. [DOI] [PubMed] [Google Scholar]
  7. Dubnau E., Maas W. K. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli. J Bacteriol. 1968 Feb;95(2):531–539. doi: 10.1128/jb.95.2.531-539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iordănescu S., Greceanu V. Localisation du déterminant de la streptomycino- résistance chez une souche de Staphylococcus aureus. Arch Roum Pathol Exp Microbiol. 1971 Sep-Dec;30(3):463–471. [PubMed] [Google Scholar]
  9. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  10. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Novick R. P., Brodsky R. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. J Mol Biol. 1972 Jul 21;68(2):285–302. doi: 10.1016/0022-2836(72)90214-8. [DOI] [PubMed] [Google Scholar]
  12. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novick R. P. Penicillinase plasmids of Staphylococcus aureus. Fed Proc. 1967 Jan-Feb;26(1):29–38. [PubMed] [Google Scholar]
  14. Oliver D. R., Manis J. J., Whitfield H. J. Evidence for a composite state of an F'his, gnd element and a cryptic plasmid in a derivative of Salmonella typhimurium LT2. J Bacteriol. 1974 Jul;119(1):192–201. doi: 10.1128/jb.119.1.192-201.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peyru G., Wexler L. F., Novick R. P. Naturally occurring penicillinase plasmids in Staphylococcus aureus. J Bacteriol. 1969 Apr;98(1):215–221. doi: 10.1128/jb.98.1.215-221.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith K., Novick R. P. Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus. J Bacteriol. 1972 Nov;112(2):761–772. doi: 10.1128/jb.112.2.761-772.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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