Abstract
Veillonella alcalescens strain ATCC 17745 was shown to require putrescine or cadaverine for growth. None of the other compounds tried, including magnesium and spermidine, were able to substitute for the diamines. Studies with labeled diamines showed that spermidine was made from putrescine in this organism. A polyamine analogous to spermidine, but made from cadaverine, was not found. A combination of growth experiments and chemical assays suggested that protein synthesis was limited in diamine-starved cells. Protein synthesis occurred prior to nucleic acid synthesis when putrescine was added to starved cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hammond J. E., Herbst E. J. Analysis of polyamines by thin-layer chromatography. Anal Biochem. 1968 Mar;22(3):474–484. doi: 10.1016/0003-2697(68)90288-1. [DOI] [PubMed] [Google Scholar]
- Hirshfield I. N., Rosenfeld H. J., Leifer Z., Maas W. K. Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine. J Bacteriol. 1970 Mar;101(3):725–730. doi: 10.1128/jb.101.3.725-730.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorstad C. M., Morris D. R. Polyamine limitation of growth slows the rate of polypeptide chain elongation in Escherichia coli. J Bacteriol. 1974 Sep;119(3):857–860. doi: 10.1128/jb.119.3.857-860.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karrer E., Bose R. J., Warren R. A. Polyamines of Pseudomonas acidovorans. J Bacteriol. 1973 Jun;114(3):1365–1366. doi: 10.1128/jb.114.3.1365-1366.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. R., Hansen M. T. Influence of polyamine limitation on the chain growth rates of beta-galactosidase and of its messenger ribonucleic acid. J Bacteriol. 1973 Nov;116(2):588–592. doi: 10.1128/jb.116.2.588-592.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. R., Jorstad C. M. Growth and macromolecular composition of a mutant of Escherichia coli during polyamine limitation. J Bacteriol. 1973 Jan;113(1):271–277. doi: 10.1128/jb.113.1.271-277.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. R., Jorstad C. M. Isolation of conditionally putrescine-deficient mutants of Escherichia coli. J Bacteriol. 1970 Mar;101(3):731–737. doi: 10.1128/jb.101.3.731-737.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Munro G. F., Bell C. A. Polyamine requirements of a conditional polyamine auxotroph of Escherichia coli. J Bacteriol. 1973 Aug;115(2):469–475. doi: 10.1128/jb.115.2.469-475.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROGOSA M., BISHOP F. S. THE GENUS VEILLONELLA . II. NUTRITIONAL STUDIES. J Bacteriol. 1964 Mar;87:574–580. doi: 10.1128/jb.87.3.574-580.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens L. The biochemical role of naturally occurring polyamines in nucleic acid synthesis. Biol Rev Camb Philos Soc. 1970 Feb;45(1):1–27. doi: 10.1111/j.1469-185x.1970.tb01073.x. [DOI] [PubMed] [Google Scholar]
- Tabor H., Tabor C. W. Biosynthesis and metabolism of 1,4-diaminobutane, spermidine, spermine, and related amines. Adv Enzymol Relat Areas Mol Biol. 1972;36:203–268. doi: 10.1002/9780470122815.ch7. [DOI] [PubMed] [Google Scholar]
- Young D. V., Srinivasan P. R. Growth of ribonucleic acid bacteriophage f2 in a conditional putrescine auxotroph of Escherichia coli: evidence for a polyamine role in translation. J Bacteriol. 1974 Mar;117(3):1280–1288. doi: 10.1128/jb.117.3.1280-1288.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young D. V., Srinivasan P. R. Regulation of macromolecular synthesis by putrescine in a conditional Escherichia coli putrescine auxotroph. J Bacteriol. 1972 Oct;112(1):30–39. doi: 10.1128/jb.112.1.30-39.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]