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. 1976 Feb;125(2):581–587. doi: 10.1128/jb.125.2.581-587.1976

Urea-hydrolyzing activity of a T-strain mycoplasma: Ureaplasma urealyticum.

G K Masover, J E Sawyer, L Hayflick
PMCID: PMC236118  PMID: 1381

Abstract

The urea-hydrolyzing activity of a T-strain mycoplasma was studied in experiments using whole cells and cell-free enzyme preparations by measuring the release of 14CO2 from [14C]urea. Under the conditions used, the urea concentration optimum is approximately 5.6 X 10(-3) M urea. The activity is soluble and not membrane bound. It is stable at -70 C for several weeks but is more labile at higher temperatures. The pH optimum is between 5.0 and 6.0. The effect of several inhibitors on the activity was tested and revealed similarities, as well as differences, between T-strain mycoplasma urease activity and the urease activity of other organisms and plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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